Relative subcellular distribution of the Atg9-GFP containing structures and Cvt complex
| Criteria/cell type | Location | |||
| Adjacent to mitochondria | Perivacuolar | Perinuclear | Other cytoplasmic | |
| Atg9-GFP–containing membranous clusters | ||||
| WT | 57.0 ± 0.2 | 3.8 ± 0.05 | 4.9 ± 0.05 | 34.3 ± 0.1 |
| atg11Δ | 50 ± 0.4 | 4 ± 0.3 | 2 ± 0.03 | 44 ± 0.1 |
| atg1Δ | 17.5 ± 0.1 | 37.5 ± 0.1 | 7.5 ± 0.05 | 37.5 ± 0.2 |
| Cvt complexes associated with Atg9-GFP–containing membranous clusters | ||||
| WT | 58.8 ± 0.3 | 2.0 ± 0.1 | 3.9 ± 0.2 | 35.3 ± 0.3 |
| atg11Δ | ND | ND | ND | ND |
| atg1Δ | 14.3 ± 0.2 | 57.1 ± 0.2 | 12.2 ± 0.5 | 16.4 ± 0.8 |
| Free Cvt complexes | ||||
| WT | 2.2 ± 0.2 | 0 | 0 | 97.8 ± 0.3 |
| atg11Δ | 15.4 ± 1.1 | 0 | 1.2 ± 0.8 | 83.4 ± 1.5 |
| atg1Δ | ND | ND | ND | ND |
| Criteria/cell type | Location | |||
| Adjacent to mitochondria | Perivacuolar | Perinuclear | Other cytoplasmic | |
| Atg9-GFP–containing membranous clusters | ||||
| WT | 57.0 ± 0.2 | 3.8 ± 0.05 | 4.9 ± 0.05 | 34.3 ± 0.1 |
| atg11Δ | 50 ± 0.4 | 4 ± 0.3 | 2 ± 0.03 | 44 ± 0.1 |
| atg1Δ | 17.5 ± 0.1 | 37.5 ± 0.1 | 7.5 ± 0.05 | 37.5 ± 0.2 |
| Cvt complexes associated with Atg9-GFP–containing membranous clusters | ||||
| WT | 58.8 ± 0.3 | 2.0 ± 0.1 | 3.9 ± 0.2 | 35.3 ± 0.3 |
| atg11Δ | ND | ND | ND | ND |
| atg1Δ | 14.3 ± 0.2 | 57.1 ± 0.2 | 12.2 ± 0.5 | 16.4 ± 0.8 |
| Free Cvt complexes | ||||
| WT | 2.2 ± 0.2 | 0 | 0 | 97.8 ± 0.3 |
| atg11Δ | 15.4 ± 1.1 | 0 | 1.2 ± 0.8 | 83.4 ± 1.5 |
| atg1Δ | ND | ND | ND | ND |
Counting and statistical analyses were carried out as described in Materials and methods. All the results are expressed in percentages ± the standard error of the mean. ND, not determined because this structure is not present or very rare in the indicated strain.