Frequency of the Atg9-GFP containing structures and the Cvt complex
| Criteria | Strain background | ||
| WT | atg11Δ | atg1Δ | |
| Atg9-GFP–containing membranous clusters/cell section | |||
| 0 | 47 ± 1.8 | 57 ± 2.3 | 84 ± 1.2 |
| 1 | 28 ± 1.3 | 25 ± 0.8 | 14 ± 0.7a |
| 2 | 19 ± 0.9 | 16 ± 0.8 | 2 ± 0.2a |
| 3 | 5 ± 0.8 | 2 ± 1.1 | 0 |
| ≥4 | 1 ± 0.2 | 0 | 0 |
| Cvt complex/cell section | |||
| 0 | 99 ± 0.1 | 99.7 ± 0.3 | 95 ± 0.8 |
| 1 | 1 ± 0.4 | 0.3 ± 0.1a | 5 ± 0.8a |
| 2 | 0 | 0 | 0 |
| 3 | 0 | 0 | 0 |
| ≥4 | 0 | 0 | 0 |
| Criteria | Strain background | ||
| WT | atg11Δ | atg1Δ | |
| Atg9-GFP–containing membranous clusters/cell section | |||
| 0 | 47 ± 1.8 | 57 ± 2.3 | 84 ± 1.2 |
| 1 | 28 ± 1.3 | 25 ± 0.8 | 14 ± 0.7a |
| 2 | 19 ± 0.9 | 16 ± 0.8 | 2 ± 0.2a |
| 3 | 5 ± 0.8 | 2 ± 1.1 | 0 |
| ≥4 | 1 ± 0.2 | 0 | 0 |
| Cvt complex/cell section | |||
| 0 | 99 ± 0.1 | 99.7 ± 0.3 | 95 ± 0.8 |
| 1 | 1 ± 0.4 | 0.3 ± 0.1a | 5 ± 0.8a |
| 2 | 0 | 0 | 0 |
| 3 | 0 | 0 | 0 |
| ≥4 | 0 | 0 | 0 |
Counting and statistical analyses were carried out as described in Materials and methods. All the results are expressed in percentages ± the standard error of the mean.
P ≤ 0.05.