[Ca2+]1/2 values for each C2 domain of otoferlin
| C2 domain | − Liposomes [Ca2+]1/2 | + Liposomes [Ca2+]1/2 |
| µM | µM | |
| C2A | ND | ND |
| C2B | 21.1 ± 1.1 | 6.7 ± 0.3 |
| C2C | 15.1 ± 2.0 | 5.4 ± 0.1 |
| C2D | 17.0 ± 1.1 | 4.4 ± 0.1 |
| C2E | 25.0 ± 1.50 | 7.5 ± 0.8 |
| C2F | 21.1 ± 2.0 | 10 ± 0.2 |
| C2ABC | 15.4 ± 0.60 | 2.0 ± 0.1 |
| C2DEF | 14.7 ± 0.60 | 1.9 ± 0.8 |
| C2 domain | − Liposomes [Ca2+]1/2 | + Liposomes [Ca2+]1/2 |
| µM | µM | |
| C2A | ND | ND |
| C2B | 21.1 ± 1.1 | 6.7 ± 0.3 |
| C2C | 15.1 ± 2.0 | 5.4 ± 0.1 |
| C2D | 17.0 ± 1.1 | 4.4 ± 0.1 |
| C2E | 25.0 ± 1.50 | 7.5 ± 0.8 |
| C2F | 21.1 ± 2.0 | 10 ± 0.2 |
| C2ABC | 15.4 ± 0.60 | 2.0 ± 0.1 |
| C2DEF | 14.7 ± 0.60 | 1.9 ± 0.8 |