Table I. Rate and extent of lysosome... | Rockefeller University Press

Table I.

Rate and extent of lysosome re-acidification after cytosolic anion dialysis and protonophore-induced alkalinization

	n	τ (min)	pH_f − pH_o	pH_alk − pH_f
Control	16	0.68 ± 0.07	−0.01 ± 0.03	0.91 ± 0.09
Gluconate⁻	6	0.44 ± 0.02	−0.03 ± 0.03	0.72 ± 0.17
		(P > 0.05; NS)	(P > 0.05; NS)	(P > 0.05; NS)
MeSO₄⁻	6	0.69 ± 0.06	0.09 ± 0.05	1.31 ± 0.05
		(P > 0.05; NS)	(P > 0.05; NS)	(P < 0.05)
CFTR_INH-172	5	1.34 ± 0.25	−0.17 ± 0.10	0.71 ± 0.07
		(P < 0.01)	(P > 0.05; NS)	(P > 0.05; NS)

	n	τ (min)	pH_f − pH_o	pH_alk − pH_f
Control	16	0.68 ± 0.07	−0.01 ± 0.03	0.91 ± 0.09
Gluconate⁻	6	0.44 ± 0.02	−0.03 ± 0.03	0.72 ± 0.17
		(P > 0.05; NS)	(P > 0.05; NS)	(P > 0.05; NS)
MeSO₄⁻	6	0.69 ± 0.06	0.09 ± 0.05	1.31 ± 0.05
		(P > 0.05; NS)	(P > 0.05; NS)	(P < 0.05)
CFTR_INH-172	5	1.34 ± 0.25	−0.17 ± 0.10	0.71 ± 0.07
		(P < 0.01)	(P > 0.05; NS)	(P > 0.05; NS)

The differences between the initial lysosome pH (pH_o; step I of the protocol shown in Fig. 3 A), pH after protonophore-induced alkalinization (pH_alk; step II of the protocol in Fig. 3 A), and final pH after re-acidification (pH_f; step III of the protocol in Fig. 3 A) were calculated. To determine the half-life of the pH decay (τ) the data for lysosome re-acidification after protonophore washout were fit to a single exponential of the form y = A * exp(−K x) + B and τ calculated as 0.69/K. All values are listed as mean ± SE. The p-values, given in parentheses, were determined by ANOVA using Dunnett’s test for multiple comparison to the control (NS, not significant). n refers to the number of experiments performed for each condition. For each experiment, 6–13 regions of interest were measured.

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