Rate and extent of lysosome re-acidification after cytosolic anion dialysis and protonophore-induced alkalinization
| n | τ (min) | pHf − pHo | pHalk − pHf | |
| Control | 16 | 0.68 ± 0.07 | −0.01 ± 0.03 | 0.91 ± 0.09 |
| Gluconate− | 6 | 0.44 ± 0.02 | −0.03 ± 0.03 | 0.72 ± 0.17 |
| (P > 0.05; NS) | (P > 0.05; NS) | (P > 0.05; NS) | ||
| MeSO4− | 6 | 0.69 ± 0.06 | 0.09 ± 0.05 | 1.31 ± 0.05 |
| (P > 0.05; NS) | (P > 0.05; NS) | (P < 0.05) | ||
| CFTRINH-172 | 5 | 1.34 ± 0.25 | −0.17 ± 0.10 | 0.71 ± 0.07 |
| (P < 0.01) | (P > 0.05; NS) | (P > 0.05; NS) |
| n | τ (min) | pHf − pHo | pHalk − pHf | |
| Control | 16 | 0.68 ± 0.07 | −0.01 ± 0.03 | 0.91 ± 0.09 |
| Gluconate− | 6 | 0.44 ± 0.02 | −0.03 ± 0.03 | 0.72 ± 0.17 |
| (P > 0.05; NS) | (P > 0.05; NS) | (P > 0.05; NS) | ||
| MeSO4− | 6 | 0.69 ± 0.06 | 0.09 ± 0.05 | 1.31 ± 0.05 |
| (P > 0.05; NS) | (P > 0.05; NS) | (P < 0.05) | ||
| CFTRINH-172 | 5 | 1.34 ± 0.25 | −0.17 ± 0.10 | 0.71 ± 0.07 |
| (P < 0.01) | (P > 0.05; NS) | (P > 0.05; NS) |
The differences between the initial lysosome pH (pHo; step I of the protocol shown in Fig. 3 A), pH after protonophore-induced alkalinization (pHalk; step II of the protocol in Fig. 3 A), and final pH after re-acidification (pHf; step III of the protocol in Fig. 3 A) were calculated. To determine the half-life of the pH decay (τ) the data for lysosome re-acidification after protonophore washout were fit to a single exponential of the form y = A * exp(−K x) + B and τ calculated as 0.69/K. All values are listed as mean ± SE. The p-values, given in parentheses, were determined by ANOVA using Dunnett’s test for multiple comparison to the control (NS, not significant). n refers to the number of experiments performed for each condition. For each experiment, 6–13 regions of interest were measured.