Defects associated with aust onion stage spermatids
| Genotype . | Number of spermatids . | Type of spermatids . | . | . | . | . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | N . | . | 2× N . | . | 4× N . | . | . | . | . | . | |||||||||
| . | . | Nu . | Nu . | 2 Nu . | 2 Nu . | Nu . | 2 Nu . | 3 Nu . | 4 Nu . | 4 Nu . | >4 Nu . | |||||||||
| A | B | A | B | A | B | |||||||||||||||
| aust/Cyo | 716 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||||||||
| aust/Df | 1288 | 0 | 0 | 0.05 | 0 | 1.25 | 4.2 | 12.5 | 4.45 | 76.45 | 1.1 | |||||||||
| Genotype . | Number of spermatids . | Type of spermatids . | . | . | . | . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | N . | . | 2× N . | . | 4× N . | . | . | . | . | . | |||||||||
| . | . | Nu . | Nu . | 2 Nu . | 2 Nu . | Nu . | 2 Nu . | 3 Nu . | 4 Nu . | 4 Nu . | >4 Nu . | |||||||||
| A | B | A | B | A | B | |||||||||||||||
| aust/Cyo | 716 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||||||||
| aust/Df | 1288 | 0 | 0 | 0.05 | 0 | 1.25 | 4.2 | 12.5 | 4.45 | 76.45 | 1.1 | |||||||||
Data represent percentage of spermatid type. 100% of wild-type spermatids possess a single nucleus and a single Nebenkern of regular size. In contrast, no spermatids in aust mutants exhibit regular morphology. Over 75% of onion stage spermatids possess a single large Nebenkern surrounded by four nuclei of irregular size. N refers to the size of the Nebenkern observed. N indicates a Nebenkern of regular size, 2× N indicates a Nebenkern twice the normal size, and 4× N indicates a Nebenkern four times the size of those seen in wild-type spermatids. Nu refers to the number of nuclei present. A and B indicate regular and irregular nuclear sizes, respectively.