Table III. Selective inhibition of... | Rockefeller University Press

Table III.

Selective inhibition of expression of AC and IP₃R isoforms

siRNA	AC3	AC6	IP₃R1	IP₃R2	IP₃R3
AC3	40 ± 2^{^a}	123 ± 35	103 ± 9	93 ± 14	87 ± 7
AC6	101 ± 27	38 ± 8^{^a}	91 ± 6	101 ± 12	92 ± 10
IP₃R1	ND	ND	36 ± 6^{^a}	103 ± 9	138 ± 7
IP₃R2	ND	ND	96 ± 18	≤5^{^a}	77 ± 6

siRNA	AC3	AC6	IP₃R1	IP₃R2	IP₃R3
AC3	40 ± 2^{^a}	123 ± 35	103 ± 9	93 ± 14	87 ± 7
AC6	101 ± 27	38 ± 8^{^a}	91 ± 6	101 ± 12	92 ± 10
IP₃R1	ND	ND	36 ± 6^{^a}	103 ± 9	138 ± 7
IP₃R2	ND	ND	96 ± 18	≤5^{^a}	77 ± 6

Quantification of levels of expression (percentage of control, means ± SEM from three to five independent transfections) of the isoforms of AC and IP₃R from HEK-PR1 cells treated with the indicated siRNA. Samples were taken from the same cells that were used for measurements of [Ca²⁺]_i and cAMP (Fig. 9). WB was used to quantify IP₃R expression and QPCR for AC (available antibodies are incapable of detecting endogenous levels of AC). ND, not determined.

Indicates the extent to which expression of the target protein was reduced.

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