Effects of PTH, FK, and isoproterenol on cAMP and potentiation of CCh-evoked Ca2+ signals
| Δ cAMP | Δ [Ca2+]i | |||||
|---|---|---|---|---|---|---|
| Maximal response | EC50 | Maximal increase | EC50 | |||
| fmol/sample | nM | |||||
| PTH | 6,662 ± 1232 | 29 ± 7 nM | 101 ± 14 | 31 ± 10 nM | ||
| FK | 236 ± 29 | 3.3 ± 0.4 μM | 55 ± 5 | 8.7 ± 2.0 μM | ||
| Isoproterenol | 91 ± 15 | 113 ± 43 nM | 30 ± 6 | 90 ± 24 nM | ||
| Δ cAMP | Δ [Ca2+]i | |||||
|---|---|---|---|---|---|---|
| Maximal response | EC50 | Maximal increase | EC50 | |||
| PTH | 6,662 ± 1232 | 29 ± 7 nM | 101 ± 14 | 31 ± 10 nM | ||
| FK | 236 ± 29 | 3.3 ± 0.4 μM | 55 ± 5 | 8.7 ± 2.0 μM | ||
| Isoproterenol | 91 ± 15 | 113 ± 43 nM | 30 ± 6 | 90 ± 24 nM | ||
Results (means ± SEM, n = 3–10) show increases in cAMP and potentiation of the CCh-evoked Ca2+ signal evoked by each stimulus, with each response measured under identical conditions after a 30-s stimulation.