Table III. Previously reported SMN... | Rockefeller University Press

Table III.

Previously reported SMN interaction partners identified in quantitative proteomics screen utilizing GFP binder to immunoprecipitate GFP-SMN complexes

	MW (kD)	Cytoplasmic SILAC Ratio ± SD	# Peptides	Nucleoplasmic SILAC Ratio ± SD	# Peptides	Reference
SMN	31.8	14.0 ± 19.0	15	76.4 ± 96.5	13	(Kolb et al., 2007; Otter et al., 2007)
Gemin 2	31.6	10.8 ± 2.2	10	39.3 ± 26.8	6	(Liu et al., 1997)
Gemin 3	92.2	74.2 ± 87.0	36	33.2 ± 35.6	33	(Charroux et al., 1999; Campbell et al., 2000)
Gemin 4	120	66.5 ± 79.5	54	36.3 ± 46.8	46	(Charroux et al., 2000)
Gemin 5	168.6	5.2 ± 1.3	26	9.9 ± 7.3	19	(Gubitz et al., 2002)
Gemin 6	18.8	50.5 ± 59.8	7	34.3 ± 43.5	6	(Pellizzoni et al., 2002a)
Gemin 7	14.5	36.8 ± 3.2	6	18 ± 12.1	5	(Baccon et al., 2002)
Gemin 8	40.1	80.3 ± 102.3	11	26.1 ± 41.6	9	(Carissimi et al., 2006)
U1A	31.3	3.5 ± 0.3	5	2.5 ± 0.2	4	(Yong et al., 2002)
SmB/B′	30	0.8 ± 0.01	5	1.9 ± 0.1	5	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmD1	13.3	0.9 ± 0.1	6	11.4 ± 2.2	4	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmD2	13.5	0.9 ± 0.1	10	8.9 ± 1.3	7	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmD3	13.9	1.0 ± 0.1	4	2.0 ± 0.2	5	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmE	10.8	1.0 ± 0.02	4	6.5 ± 0.6	4	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmF	9.7	1.0 ± 0.1	3	5.6 ± 0.03	4	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmG	8.5	1.0 ± 0.04	6	6.6 ± 1.4	4	(Liu et al., 1997; Pellizzoni et al., 2002b)
Lsm10	14.1	5.8 ± 2.5	7	7.8 ± 2.7	5	(Pillai et al., 2001, 2003)
Lsm11	39.5	6.7 ± 1.7	11	0.7 ± 0.1	1	(Pillai et al., 2003)
Unrip	38.4	1.4 ± 0.1	11	3.6 ± 0.9	17	(Carissimi et al., 2005)
Coilin	62.6	-	-	21.6 ± 13.0	14	(Hebert et al., 2001)
PRMT5	72.7	1.5 ± 0.3	5	-	-	(Meister and Fischer, 2002)
Fibrillarin	33.8	-	-	2.8 ± 0.5	1	(Liu and Dreyfuss, 1996; Jones et al., 2001; Pellizzoni et al., 2001a)
hnRNP U	90.6	1.7 ± 0.1	8	3.9 ± 0.8	13	(Liu and Dreyfuss, 1996)

	MW (kD)	Cytoplasmic SILAC Ratio ± SD	# Peptides	Nucleoplasmic SILAC Ratio ± SD	# Peptides	Reference
SMN	31.8	14.0 ± 19.0	15	76.4 ± 96.5	13	(Kolb et al., 2007; Otter et al., 2007)
Gemin 2	31.6	10.8 ± 2.2	10	39.3 ± 26.8	6	(Liu et al., 1997)
Gemin 3	92.2	74.2 ± 87.0	36	33.2 ± 35.6	33	(Charroux et al., 1999; Campbell et al., 2000)
Gemin 4	120	66.5 ± 79.5	54	36.3 ± 46.8	46	(Charroux et al., 2000)
Gemin 5	168.6	5.2 ± 1.3	26	9.9 ± 7.3	19	(Gubitz et al., 2002)
Gemin 6	18.8	50.5 ± 59.8	7	34.3 ± 43.5	6	(Pellizzoni et al., 2002a)
Gemin 7	14.5	36.8 ± 3.2	6	18 ± 12.1	5	(Baccon et al., 2002)
Gemin 8	40.1	80.3 ± 102.3	11	26.1 ± 41.6	9	(Carissimi et al., 2006)
U1A	31.3	3.5 ± 0.3	5	2.5 ± 0.2	4	(Yong et al., 2002)
SmB/B′	30	0.8 ± 0.01	5	1.9 ± 0.1	5	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmD1	13.3	0.9 ± 0.1	6	11.4 ± 2.2	4	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmD2	13.5	0.9 ± 0.1	10	8.9 ± 1.3	7	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmD3	13.9	1.0 ± 0.1	4	2.0 ± 0.2	5	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmE	10.8	1.0 ± 0.02	4	6.5 ± 0.6	4	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmF	9.7	1.0 ± 0.1	3	5.6 ± 0.03	4	(Liu et al., 1997; Pellizzoni et al., 2002b)
SmG	8.5	1.0 ± 0.04	6	6.6 ± 1.4	4	(Liu et al., 1997; Pellizzoni et al., 2002b)
Lsm10	14.1	5.8 ± 2.5	7	7.8 ± 2.7	5	(Pillai et al., 2001, 2003)
Lsm11	39.5	6.7 ± 1.7	11	0.7 ± 0.1	1	(Pillai et al., 2003)
Unrip	38.4	1.4 ± 0.1	11	3.6 ± 0.9	17	(Carissimi et al., 2005)
Coilin	62.6	-	-	21.6 ± 13.0	14	(Hebert et al., 2001)
PRMT5	72.7	1.5 ± 0.3	5	-	-	(Meister and Fischer, 2002)
Fibrillarin	33.8	-	-	2.8 ± 0.5	1	(Liu and Dreyfuss, 1996; Jones et al., 2001; Pellizzoni et al., 2001a)
hnRNP U	90.6	1.7 ± 0.1	8	3.9 ± 0.8	13	(Liu and Dreyfuss, 1996)

This table summarizes the protein interaction datasets collected from both cytoplasmic and nuclear extracts using the GFP binder to pull down GFP-SMN from stable HeLa cell lines. Both the SILAC ratio (with SD) and the number of peptides identified for each protein in a particular experiment are indicated. Ratios >10, which inevitably show higher standard deviations, as discussed in the text, are in bold. References for the initial characterization of each protein as an SMN interaction partner are also listed.

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