Previously reported SMN interaction partners identified in quantitative proteomics screen utilizing GFP binder to immunoprecipitate GFP-SMN complexes
. | MW (kD) . | Cytoplasmic SILAC Ratio ± SD . | # Peptides . | Nucleoplasmic SILAC Ratio ± SD . | # Peptides . | Reference . |
|---|---|---|---|---|---|---|
| SMN | 31.8 | 14.0 ± 19.0 | 15 | 76.4 ± 96.5 | 13 | (Kolb et al., 2007; Otter et al., 2007) |
| Gemin 2 | 31.6 | 10.8 ± 2.2 | 10 | 39.3 ± 26.8 | 6 | (Liu et al., 1997) |
| Gemin 3 | 92.2 | 74.2 ± 87.0 | 36 | 33.2 ± 35.6 | 33 | (Charroux et al., 1999; Campbell et al., 2000) |
| Gemin 4 | 120 | 66.5 ± 79.5 | 54 | 36.3 ± 46.8 | 46 | (Charroux et al., 2000) |
| Gemin 5 | 168.6 | 5.2 ± 1.3 | 26 | 9.9 ± 7.3 | 19 | (Gubitz et al., 2002) |
| Gemin 6 | 18.8 | 50.5 ± 59.8 | 7 | 34.3 ± 43.5 | 6 | (Pellizzoni et al., 2002a) |
| Gemin 7 | 14.5 | 36.8 ± 3.2 | 6 | 18 ± 12.1 | 5 | (Baccon et al., 2002) |
| Gemin 8 | 40.1 | 80.3 ± 102.3 | 11 | 26.1 ± 41.6 | 9 | (Carissimi et al., 2006) |
| U1A | 31.3 | 3.5 ± 0.3 | 5 | 2.5 ± 0.2 | 4 | (Yong et al., 2002) |
| SmB/B′ | 30 | 0.8 ± 0.01 | 5 | 1.9 ± 0.1 | 5 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmD1 | 13.3 | 0.9 ± 0.1 | 6 | 11.4 ± 2.2 | 4 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmD2 | 13.5 | 0.9 ± 0.1 | 10 | 8.9 ± 1.3 | 7 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmD3 | 13.9 | 1.0 ± 0.1 | 4 | 2.0 ± 0.2 | 5 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmE | 10.8 | 1.0 ± 0.02 | 4 | 6.5 ± 0.6 | 4 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmF | 9.7 | 1.0 ± 0.1 | 3 | 5.6 ± 0.03 | 4 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmG | 8.5 | 1.0 ± 0.04 | 6 | 6.6 ± 1.4 | 4 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| Lsm10 | 14.1 | 5.8 ± 2.5 | 7 | 7.8 ± 2.7 | 5 | (Pillai et al., 2001, 2003) |
| Lsm11 | 39.5 | 6.7 ± 1.7 | 11 | 0.7 ± 0.1 | 1 | (Pillai et al., 2003) |
| Unrip | 38.4 | 1.4 ± 0.1 | 11 | 3.6 ± 0.9 | 17 | (Carissimi et al., 2005) |
| Coilin | 62.6 | - | - | 21.6 ± 13.0 | 14 | (Hebert et al., 2001) |
| PRMT5 | 72.7 | 1.5 ± 0.3 | 5 | - | - | (Meister and Fischer, 2002) |
| Fibrillarin | 33.8 | - | - | 2.8 ± 0.5 | 1 | (Liu and Dreyfuss, 1996; Jones et al., 2001; Pellizzoni et al., 2001a) |
| hnRNP U | 90.6 | 1.7 ± 0.1 | 8 | 3.9 ± 0.8 | 13 | (Liu and Dreyfuss, 1996) |
. | MW (kD) . | Cytoplasmic SILAC Ratio ± SD . | # Peptides . | Nucleoplasmic SILAC Ratio ± SD . | # Peptides . | Reference . |
|---|---|---|---|---|---|---|
| SMN | 31.8 | 14.0 ± 19.0 | 15 | 76.4 ± 96.5 | 13 | (Kolb et al., 2007; Otter et al., 2007) |
| Gemin 2 | 31.6 | 10.8 ± 2.2 | 10 | 39.3 ± 26.8 | 6 | (Liu et al., 1997) |
| Gemin 3 | 92.2 | 74.2 ± 87.0 | 36 | 33.2 ± 35.6 | 33 | (Charroux et al., 1999; Campbell et al., 2000) |
| Gemin 4 | 120 | 66.5 ± 79.5 | 54 | 36.3 ± 46.8 | 46 | (Charroux et al., 2000) |
| Gemin 5 | 168.6 | 5.2 ± 1.3 | 26 | 9.9 ± 7.3 | 19 | (Gubitz et al., 2002) |
| Gemin 6 | 18.8 | 50.5 ± 59.8 | 7 | 34.3 ± 43.5 | 6 | (Pellizzoni et al., 2002a) |
| Gemin 7 | 14.5 | 36.8 ± 3.2 | 6 | 18 ± 12.1 | 5 | (Baccon et al., 2002) |
| Gemin 8 | 40.1 | 80.3 ± 102.3 | 11 | 26.1 ± 41.6 | 9 | (Carissimi et al., 2006) |
| U1A | 31.3 | 3.5 ± 0.3 | 5 | 2.5 ± 0.2 | 4 | (Yong et al., 2002) |
| SmB/B′ | 30 | 0.8 ± 0.01 | 5 | 1.9 ± 0.1 | 5 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmD1 | 13.3 | 0.9 ± 0.1 | 6 | 11.4 ± 2.2 | 4 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmD2 | 13.5 | 0.9 ± 0.1 | 10 | 8.9 ± 1.3 | 7 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmD3 | 13.9 | 1.0 ± 0.1 | 4 | 2.0 ± 0.2 | 5 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmE | 10.8 | 1.0 ± 0.02 | 4 | 6.5 ± 0.6 | 4 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmF | 9.7 | 1.0 ± 0.1 | 3 | 5.6 ± 0.03 | 4 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| SmG | 8.5 | 1.0 ± 0.04 | 6 | 6.6 ± 1.4 | 4 | (Liu et al., 1997; Pellizzoni et al., 2002b) |
| Lsm10 | 14.1 | 5.8 ± 2.5 | 7 | 7.8 ± 2.7 | 5 | (Pillai et al., 2001, 2003) |
| Lsm11 | 39.5 | 6.7 ± 1.7 | 11 | 0.7 ± 0.1 | 1 | (Pillai et al., 2003) |
| Unrip | 38.4 | 1.4 ± 0.1 | 11 | 3.6 ± 0.9 | 17 | (Carissimi et al., 2005) |
| Coilin | 62.6 | - | - | 21.6 ± 13.0 | 14 | (Hebert et al., 2001) |
| PRMT5 | 72.7 | 1.5 ± 0.3 | 5 | - | - | (Meister and Fischer, 2002) |
| Fibrillarin | 33.8 | - | - | 2.8 ± 0.5 | 1 | (Liu and Dreyfuss, 1996; Jones et al., 2001; Pellizzoni et al., 2001a) |
| hnRNP U | 90.6 | 1.7 ± 0.1 | 8 | 3.9 ± 0.8 | 13 | (Liu and Dreyfuss, 1996) |
This table summarizes the protein interaction datasets collected from both cytoplasmic and nuclear extracts using the GFP binder to pull down GFP-SMN from stable HeLa cell lines. Both the SILAC ratio (with SD) and the number of peptides identified for each protein in a particular experiment are indicated. Ratios >10, which inevitably show higher standard deviations, as discussed in the text, are in bold. References for the initial characterization of each protein as an SMN interaction partner are also listed.