Sepharose bead proteome: most common protein classes
| Protein class . | Most commonly found . |
|---|---|
| Cytoskeletal/structural/ motility proteins | Actin |
| Cofilin | |
| Desmin | |
| Desmoplakin | |
| Epiplakin | |
| Filamin | |
| Myosin | |
| Peripherin | |
| Plectin | |
| Tropomyosin | |
| Tubulin | |
| Vimentin | |
| DEAD box proteins | |
| Eukaryotic translation elongation and initiation factors | |
| Heat shock proteins | |
| Histones | |
| hnRNP proteins | |
| Ribosomal proteins |
| Protein class . | Most commonly found . |
|---|---|
| Cytoskeletal/structural/ motility proteins | Actin |
| Cofilin | |
| Desmin | |
| Desmoplakin | |
| Epiplakin | |
| Filamin | |
| Myosin | |
| Peripherin | |
| Plectin | |
| Tropomyosin | |
| Tubulin | |
| Vimentin | |
| DEAD box proteins | |
| Eukaryotic translation elongation and initiation factors | |
| Heat shock proteins | |
| Histones | |
| hnRNP proteins | |
| Ribosomal proteins |
Proteins that bind nonspecifically to Sepharose fall into several distinct classes, as shown here, and are found in nearly every SILAC immunoprecipitation experiment carried out using Sepharose as an affinity matrix.