List of fission yeast strains constructed in this study
| Name . | Genotype . |
|---|---|
| MBY4489 | h− pak1-2xMyc-GFP∷ura4+ |
| MBY5064 | h− pak1-M460G-2xMyc-GFP∷ura4+ |
| MBY4694 | h+ pak1-2xMyc-GFP∷ura4+ sid4-GFP∷kanR |
| MBY5163 | h− pak1-2xMyc-GFP∷ura4+ rlc1-Cherry∷ura4+ |
| MBY5187 | h− cps1-191 pak1-2xMyc-GFP∷ura4+ rlc1-Cherry∷ura4+ |
| MBY4542 | h− nda3-KM311 pak1-2xMyc-GFP∷ura4+ |
| MBY5252 | h− nda3-KM311 pak1-M460G-2xMyc-GFP∷ura4+ |
| MBY5177 | h+ cdc25-22 rlc1-GFP∷ura4+ sid4-GFP∷kanR |
| MBY5178 | h+ cdc25-22 pak1-M460G∷ura4+ rlc1-GFP∷ura4+ sid4-GFP∷kanR |
| MBY5290 | h+ nda3-KM311 pak1-2xMyc-GFP∷ura4+ rlc1-GFP∷ura4+ |
| MBY5291 | h+ nda3-KM311 pak1-M460G-2xMyc-GFP∷ura4+ rlc1-GFP∷ura4+ |
| MBY5286 | h− nda3-KM311 pak1-2xMyc-GFP∷ura4+ rlc1∷ ura4+ |
| MBY5175 | h− cdc25-22 rlc1-MYC∷ura4+ sid4-GFP∷kanR |
| MBY5176 | h− cdc25-22 pak1-M460G∷ura4+ rlc1-Myc∷ura4+ sid4-GFP∷kanR |
| MBY5706 | h+ nda3-KM311 nmt1-3xHA-pak1-13xMyc |
| MBY5678 | h− ase1∷ura4+ rlc1-GFP∷ura4+ |
| MBY5679 | h− ase1∷ura4+ rlc1-S35AS36A-GFP∷ura4+ |
| MBY5670 | h− nmt1-3xHA-pak1-13xMyc |
| MBY5671 | h− ase1∷ura4+ nmt1-3xHA-pak1-13xMyc |
| MBY5693 | h+ ase1∷ura4+ rlc1-S35AS36A-GFP∷ura4+ uch2-GFP∷ura4+ |
| MBY5698 | h− ase1∷ura4+ rlc1-GFP∷ura4+ uch2-GFP∷ura4+ |
| MBY5001 | h− pak1-K415R-2xMyc-GFP∷ura4+ |
| MBY3451 | h− nmt1-3xHA-pak1 |
| MBY3540 | h− nmt1-3xHA-pak1-K415R |
| Name . | Genotype . |
|---|---|
| MBY4489 | h− pak1-2xMyc-GFP∷ura4+ |
| MBY5064 | h− pak1-M460G-2xMyc-GFP∷ura4+ |
| MBY4694 | h+ pak1-2xMyc-GFP∷ura4+ sid4-GFP∷kanR |
| MBY5163 | h− pak1-2xMyc-GFP∷ura4+ rlc1-Cherry∷ura4+ |
| MBY5187 | h− cps1-191 pak1-2xMyc-GFP∷ura4+ rlc1-Cherry∷ura4+ |
| MBY4542 | h− nda3-KM311 pak1-2xMyc-GFP∷ura4+ |
| MBY5252 | h− nda3-KM311 pak1-M460G-2xMyc-GFP∷ura4+ |
| MBY5177 | h+ cdc25-22 rlc1-GFP∷ura4+ sid4-GFP∷kanR |
| MBY5178 | h+ cdc25-22 pak1-M460G∷ura4+ rlc1-GFP∷ura4+ sid4-GFP∷kanR |
| MBY5290 | h+ nda3-KM311 pak1-2xMyc-GFP∷ura4+ rlc1-GFP∷ura4+ |
| MBY5291 | h+ nda3-KM311 pak1-M460G-2xMyc-GFP∷ura4+ rlc1-GFP∷ura4+ |
| MBY5286 | h− nda3-KM311 pak1-2xMyc-GFP∷ura4+ rlc1∷ ura4+ |
| MBY5175 | h− cdc25-22 rlc1-MYC∷ura4+ sid4-GFP∷kanR |
| MBY5176 | h− cdc25-22 pak1-M460G∷ura4+ rlc1-Myc∷ura4+ sid4-GFP∷kanR |
| MBY5706 | h+ nda3-KM311 nmt1-3xHA-pak1-13xMyc |
| MBY5678 | h− ase1∷ura4+ rlc1-GFP∷ura4+ |
| MBY5679 | h− ase1∷ura4+ rlc1-S35AS36A-GFP∷ura4+ |
| MBY5670 | h− nmt1-3xHA-pak1-13xMyc |
| MBY5671 | h− ase1∷ura4+ nmt1-3xHA-pak1-13xMyc |
| MBY5693 | h+ ase1∷ura4+ rlc1-S35AS36A-GFP∷ura4+ uch2-GFP∷ura4+ |
| MBY5698 | h− ase1∷ura4+ rlc1-GFP∷ura4+ uch2-GFP∷ura4+ |
| MBY5001 | h− pak1-K415R-2xMyc-GFP∷ura4+ |
| MBY3451 | h− nmt1-3xHA-pak1 |
| MBY3540 | h− nmt1-3xHA-pak1-K415R |