Table I.

List of fission yeast strains constructed in this study

Name
Genotype
MBY4489 h− pak1-2xMyc-GFP∷ura4+ 
MBY5064 h− pak1-M460G-2xMyc-GFP∷ura4+ 
MBY4694 h+ pak1-2xMyc-GFP∷ura4+ sid4-GFP∷kanR 
MBY5163 h− pak1-2xMyc-GFP∷ura4+ rlc1-Cherry∷ura4+ 
MBY5187 h− cps1-191 pak1-2xMyc-GFP∷ura4+ rlc1-Cherry∷ura4+ 
MBY4542 h− nda3-KM311 pak1-2xMyc-GFP∷ura4+ 
MBY5252 h− nda3-KM311 pak1-M460G-2xMyc-GFP∷ura4+ 
MBY5177 h+ cdc25-22 rlc1-GFP∷ura4+ sid4-GFP∷kanR 
MBY5178 h+ cdc25-22 pak1-M460G∷ura4+ rlc1-GFP∷ura4+ sid4-GFP∷kanR 
MBY5290 h+ nda3-KM311 pak1-2xMyc-GFP∷ura4+ rlc1-GFP∷ura4+ 
MBY5291 h+ nda3-KM311 pak1-M460G-2xMyc-GFP∷ura4+ rlc1-GFP∷ura4+ 
MBY5286 h− nda3-KM311 pak1-2xMyc-GFP∷ura4+ rlc1∷ ura4+ 
MBY5175 h− cdc25-22 rlc1-MYC∷ura4+ sid4-GFP∷kanR 
MBY5176 h− cdc25-22 pak1-M460G∷ura4+ rlc1-Myc∷ura4+ sid4-GFP∷kanR 
MBY5706 h+ nda3-KM311 nmt1-3xHA-pak1-13xMyc 
MBY5678 h− ase1∷ura4+ rlc1-GFP∷ura4+ 
MBY5679 h− ase1∷ura4+ rlc1-S35AS36A-GFP∷ura4+ 
MBY5670 h− nmt1-3xHA-pak1-13xMyc 
MBY5671 h− ase1∷ura4+ nmt1-3xHA-pak1-13xMyc 
MBY5693 h+ ase1∷ura4+ rlc1-S35AS36A-GFP∷ura4+ uch2-GFP∷ura4+ 
MBY5698 h− ase1∷ura4+ rlc1-GFP∷ura4+ uch2-GFP∷ura4+ 
MBY5001 h− pak1-K415R-2xMyc-GFP∷ura4+ 
MBY3451 h− nmt1-3xHA-pak1 
MBY3540 h− nmt1-3xHA-pak1-K415R 
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