PCR primers used for making expression constructs
| Constructs . | Forward primer . | Reverse primer . |
|---|---|---|
| Cosmc-HA | 5′-GCGGATCCACCATGCTTTCTGAAAGCAGC TCCTTTTTG-3′ | 5′-GCTCTAGACTAAGCGTAGTCTGGGACGTCGTATGGGTAG TCATTGTCAGAACCATTTG-3′ (containing HA-tag sequence) |
| HPC4-Cosmc | 5′-GCGGATCCACCATGCTTGAGGACCAGGTGGA CCCCAGGCTGATCGACGGCAAGATGCTTTCTGA AAGCAGCTCC-3′ (containing HPC4-tag sequence) | 5′-GGTCTCCAGATTTTATAGTGTGGC-3′ |
| HPC4-sCosmc-KDEL | 5′-TCCTTGCACGCCCCACTACG-3′ | 5′-GCTCTAGAGTTCATCTTTGTCATTGTCAGAACCATTTG-3′ (containing KDEL sequence) |
| Constructs . | Forward primer . | Reverse primer . |
|---|---|---|
| Cosmc-HA | 5′-GCGGATCCACCATGCTTTCTGAAAGCAGC TCCTTTTTG-3′ | 5′-GCTCTAGACTAAGCGTAGTCTGGGACGTCGTATGGGTAG TCATTGTCAGAACCATTTG-3′ (containing HA-tag sequence) |
| HPC4-Cosmc | 5′-GCGGATCCACCATGCTTGAGGACCAGGTGGA CCCCAGGCTGATCGACGGCAAGATGCTTTCTGA AAGCAGCTCC-3′ (containing HPC4-tag sequence) | 5′-GGTCTCCAGATTTTATAGTGTGGC-3′ |
| HPC4-sCosmc-KDEL | 5′-TCCTTGCACGCCCCACTACG-3′ | 5′-GCTCTAGAGTTCATCTTTGTCATTGTCAGAACCATTTG-3′ (containing KDEL sequence) |