Summary of speed and diffusion constants measured for kinesin-5 under different experimental conditions
| Buffer (70 mM Pipes + KCl added) . | ATP . | . | . | . | . | . | ADP . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | MD . | . | . | MSD . | . | . | MSD . | . | ||||||
| v (nm/s) | D (×103 nm2/s) | n | v (nm/s) | D (×103 nm2/s) | n | D (×103 nm2/s) | n | |||||||
| 0 mM | 8.9 ± 0.1 | 0.67 ± 0.02 | 77 | 9.1 ± 0.6 | 0.63 ± 0.06 | 77 | 1.06 ± 0.06 | 72 | ||||||
| 20 mM | 10.1 ± 0.3 | 1.34 ± 0.04 | 62 | 11 ± 1 | 1.2 ± 0.2 | 62 | 1.18 ± 0.06 | 66 | ||||||
| 40 mM | 0.0 ± 0.40 | 2.38 ± 0.07 | 50 | 0 ± 3 × 104 | 2.6 ± 0.2 | 81 | 3.3 ± 0.1 | 60 | ||||||
| 60 mM | 0.3 ± 0.6 | 3.3 ± 0.1 | 48 | 0 ± 1 × 106 | 3.8 ± 0.3 | 123 | 3.8 ± 0.2 | 103 | ||||||
| 80 mM | ND | ND | NA | 3.1 ± 8.1 | 3.6 ± 0.3 | 60 | 3.3 ± 0.3a | 90 | ||||||
| 60 mM (axonemes) | 23.0 ± 0.4 | 2.39 ± 0.07 | 42 | 26.0 ± 1.3 | 1.8 ± 0.3 | 42 | 1.29 ± 0.09 | 54 | ||||||
| Buffer (70 mM Pipes + KCl added) . | ATP . | . | . | . | . | . | ADP . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | MD . | . | . | MSD . | . | . | MSD . | . | ||||||
| v (nm/s) | D (×103 nm2/s) | n | v (nm/s) | D (×103 nm2/s) | n | D (×103 nm2/s) | n | |||||||
| 0 mM | 8.9 ± 0.1 | 0.67 ± 0.02 | 77 | 9.1 ± 0.6 | 0.63 ± 0.06 | 77 | 1.06 ± 0.06 | 72 | ||||||
| 20 mM | 10.1 ± 0.3 | 1.34 ± 0.04 | 62 | 11 ± 1 | 1.2 ± 0.2 | 62 | 1.18 ± 0.06 | 66 | ||||||
| 40 mM | 0.0 ± 0.40 | 2.38 ± 0.07 | 50 | 0 ± 3 × 104 | 2.6 ± 0.2 | 81 | 3.3 ± 0.1 | 60 | ||||||
| 60 mM | 0.3 ± 0.6 | 3.3 ± 0.1 | 48 | 0 ± 1 × 106 | 3.8 ± 0.3 | 123 | 3.8 ± 0.2 | 103 | ||||||
| 80 mM | ND | ND | NA | 3.1 ± 8.1 | 3.6 ± 0.3 | 60 | 3.3 ± 0.3a | 90 | ||||||
| 60 mM (axonemes) | 23.0 ± 0.4 | 2.39 ± 0.07 | 42 | 26.0 ± 1.3 | 1.8 ± 0.3 | 42 | 1.29 ± 0.09 | 54 | ||||||
NA, not applicable.
Fit to first 5 s.