Composition of dynein tail–decorated kinetochores
| Protein . | (−) Nocodazole . | (+) Nocodazole . |
|---|---|---|
| Dynein motor | − | − |
| p150Glued | ++ | + |
| CLIP170 | + | + |
| CLASP1 | ++ | + |
| LIS1 | ND | + |
| NudE | ND | + |
| ZW10 | ND | + |
| Hec1 | + | + |
| Aurora B | + | + |
| Kif2b | − | + |
| MCAK | −a | + |
| Protein . | (−) Nocodazole . | (+) Nocodazole . |
|---|---|---|
| Dynein motor | − | − |
| p150Glued | ++ | + |
| CLIP170 | + | + |
| CLASP1 | ++ | + |
| LIS1 | ND | + |
| NudE | ND | + |
| ZW10 | ND | + |
| Hec1 | + | + |
| Aurora B | + | + |
| Kif2b | − | + |
| MCAK | −a | + |
A summary of results on the localization of kinetochore proteins in the presence of dynein tail. (−) Nocodazole data refer only to aligned kinetochores.
Despite reported kinetochore localization, we observe most MCAK at spindle poles in control and tail-expressing late prometaphase/metaphase cells.