Summary of protein interactions and FRET efficiencies analyzed by FLIM in nucleoplasm and nuclear speckles in the absence or presence of an inhibitor of transcription
| . | Transcription . | . | Transcription inhibition . | . | ||
|---|---|---|---|---|---|---|
| Interaction partners . | Nucleoplasm . | Speckles . | Nucleoplasm . | Speckles . | ||
| U1 70K–SF2/ASF | ++ | +++ | − | +++ | ||
| U1 70K–SC35 | +++ | ++ | ++ | +++ | ||
| U1 70K–U2AF35 | − | − | − | − | ||
| U2AF 35–SF2/ASF | ++ | +++ | − | +++ | ||
| U2AF 35–SC35 | +++ | ++ | ++ | +++ | ||
| HCC1–U2AF35 | ++ | + | ++ | ++ | ||
| HCC1–U2AF65 | ++ | + | +++ | ++ | ||
| . | Transcription . | . | Transcription inhibition . | . | ||
|---|---|---|---|---|---|---|
| Interaction partners . | Nucleoplasm . | Speckles . | Nucleoplasm . | Speckles . | ||
| U1 70K–SF2/ASF | ++ | +++ | − | +++ | ||
| U1 70K–SC35 | +++ | ++ | ++ | +++ | ||
| U1 70K–U2AF35 | − | − | − | − | ||
| U2AF 35–SF2/ASF | ++ | +++ | − | +++ | ||
| U2AF 35–SC35 | +++ | ++ | ++ | +++ | ||
| HCC1–U2AF35 | ++ | + | ++ | ++ | ||
| HCC1–U2AF65 | ++ | + | +++ | ++ | ||
The + and − represent the strength of the protein interactions. +++, strong interaction; ++, moderate interaction; +, weak interaction; −, no interaction.