Different mph1 mutations affect differently in GCR generated by the rad5Δ mutation
| Strain number . | Plasmid . | Mph1 . | GCR rate CANr–5-FOAr . |
|---|---|---|---|
| YKJM4810 | pRS313 (HIS3) | Deletion | <3.4 × 10−9 (9) |
| YKJM1386 | None | Wild type | 9.0 × 10−8 (257) |
| YKJM4808 | pKJM582 (HIS3) | H212D | 1.4 × 10−8 (40) |
| YKJM4806 | pKJM588 (HIS3) | Q603D | 1.2 × 10−8 (34) |
| YKJM4804 | pKJM584 (HIS3) | E210Q | 1.8 × 10−8 (51) |
| YKJM4802 | pKJM586 (HIS3) | D209N | 1.7 × 10−8 (49) |
| YKJM4800 | pKJM590 (HIS3) | K113Q | 2.8 × 10−8 (80) |
| YKJM4825 | p41k-CYC (KAN) | Deletion | <2.1 × 10−9 (6) |
| YKJM4827 | pKJM781 (KAN) | Wild type | 6.3 × 10−8 (180) |
| YKJM4829 | pKJM937 (KAN) | CΔ | <1.6 × 10−9 (5) |
| Strain number . | Plasmid . | Mph1 . | GCR rate CANr–5-FOAr . |
|---|---|---|---|
| YKJM4810 | pRS313 (HIS3) | Deletion | <3.4 × 10−9 (9) |
| YKJM1386 | None | Wild type | 9.0 × 10−8 (257) |
| YKJM4808 | pKJM582 (HIS3) | H212D | 1.4 × 10−8 (40) |
| YKJM4806 | pKJM588 (HIS3) | Q603D | 1.2 × 10−8 (34) |
| YKJM4804 | pKJM584 (HIS3) | E210Q | 1.8 × 10−8 (51) |
| YKJM4802 | pKJM586 (HIS3) | D209N | 1.7 × 10−8 (49) |
| YKJM4800 | pKJM590 (HIS3) | K113Q | 2.8 × 10−8 (80) |
| YKJM4825 | p41k-CYC (KAN) | Deletion | <2.1 × 10−9 (6) |
| YKJM4827 | pKJM781 (KAN) | Wild type | 6.3 × 10−8 (180) |
| YKJM4829 | pKJM937 (KAN) | CΔ | <1.6 × 10−9 (5) |
YKJM4800, 4802, 4804, 4806, 4808, and 4810 strains are isogenic (MATa, ura3-52, leu2Δ1, trp1Δ63, his3Δ200, lys2ΔBgl, hom3-10, ade2Δ1, ade8, hxt13∷URA3, mph1∷KAN, rad5∷TRP1) with the exception of the plasmid expressing different Mph1 indicated in the plasmid and Mph1 columns. YKJM1386 is isogenic, with the exception of carrying wild-type Mph1 in the genome. YKJM4825, 4827, and 4829 strains are isogenic (MATa, ura3-52, leu2Δ1, trp1Δ63, his3Δ200, lys2ΔBgl, hom3-10, ade2Δ1, ade8, hxt13∷URA3, mph1∷TRP1, rad5∷HIS3) except for the transformed plasmid expressing Mph1 indicated in the plasmid and Mph1 columns. Numbers in parentheses in the plasmid column indicate the marker in the plasmid used. Numbers in parentheses in the GCR rate column indicate the rate relative to the wild type. CANr–5-FOAr, canavanine- and 5-FOA–resistant.