CW Δ-mtDNA/FLP Δ-mtDNA colocalization in FC cells
. | ImageJ colocalization . | Normalized colocalizationa . |
|---|---|---|
| % ± SD | % | |
| WT | 58 ± 10 (n = 14) | 100 |
| FC I | ||
| Day 22 | 18 ± 7 (n = 10) | 31 |
| Day 32 | 3 ± 3 (n = 10) | 5 |
| Day 51 | 7 ± 9 (n = 10) | 12 |
| Day 59 | 11 ± 7 (n = 9) | 19 |
| Day 104 | 4 ± 3 (n = 10) | 7 |
| Day 274 | 2 ± 2 (n = 11) | 3 |
| FC II | ||
| Day 22 | 16 ± 9 (n = 11) | 28 |
| Day 32 | 2 ± 4 (n = 10) | 4 |
| Day 60 | 2 ± 2 (n = 10) | 4 |
| Day 90 | 7 ± 4 (n = 9) | 12 |
| Day 120 | 7 ± 6 (n = 8) | 12 |
| Day 150 | 1 ± 2 (n = 10) | 2 |
. | ImageJ colocalization . | Normalized colocalizationa . |
|---|---|---|
| % ± SD | % | |
| WT | 58 ± 10 (n = 14) | 100 |
| FC I | ||
| Day 22 | 18 ± 7 (n = 10) | 31 |
| Day 32 | 3 ± 3 (n = 10) | 5 |
| Day 51 | 7 ± 9 (n = 10) | 12 |
| Day 59 | 11 ± 7 (n = 9) | 19 |
| Day 104 | 4 ± 3 (n = 10) | 7 |
| Day 274 | 2 ± 2 (n = 11) | 3 |
| FC II | ||
| Day 22 | 16 ± 9 (n = 11) | 28 |
| Day 32 | 2 ± 4 (n = 10) | 4 |
| Day 60 | 2 ± 2 (n = 10) | 4 |
| Day 90 | 7 ± 4 (n = 9) | 12 |
| Day 120 | 7 ± 6 (n = 8) | 12 |
| Day 150 | 1 ± 2 (n = 10) | 2 |
Red and green images were thresholded to remove irrelevant pixel intensities (see Materials and methods) and the proportion of pixels carrying meaningful signal for both red and green were expressed as a percentage of the total. n = number of images analyzed for each timepoint (see Fig. 7 for examples).
Colocalization expressed as a percentage of WT colocalization.