Table I. FC cell heteroplasmy as scored... | Rockefeller University Press

Table I.

FC cell heteroplasmy as scored by FISH

	Homoplasmic CWΔ	Heteroplasmic CWΔ:FLPΔ	Homoplasmic FLPΔ	Number of cells examined
	%	%	%
FC I
Day 22	0	100	0	93
Day 32	55	3	42	109
Day 51	46	4	50	172
Day 59	12	41	47	116
Day 104	42	55	3	121
FC II
Day 22	3	78	18	132
Day 25	19	50	31	74
Day 28	42	16	41	93
Day 30	54	6	40	110
Day 32	49	1	50	156
Day 60	47	9	44	115
Day 90	22	46	32	122
Day 120	40	12	48	130

	Homoplasmic CWΔ	Heteroplasmic CWΔ:FLPΔ	Homoplasmic FLPΔ	Number of cells examined
	%	%	%
FC I
Day 22	0	100	0	93
Day 32	55	3	42	109
Day 51	46	4	50	172
Day 59	12	41	47	116
Day 104	42	55	3	121
FC II
Day 22	3	78	18	132
Day 25	19	50	31	74
Day 28	42	16	41	93
Day 30	54	6	40	110
Day 32	49	1	50	156
Day 60	47	9	44	115
Day 90	22	46	32	122
Day 120	40	12	48	130

Cells for both fusion experiments (FC I and FC II) were scored as heteroplasmic CWΔ:FLPΔ if they carried appreciable FISH signal for both CW Δ-mtDNA (red) and FLP Δ-mtDNA (green) FISH probes. Cells having predominantly one signal were scored as homoplasmic. For example, the arrowheads in Fig. 6 B at day 120 show nucleoids that are in a clear minority (i.e. red in a predominantly green cell, green in a predominantly red cell). Both cells were considered to be essentially homoplasmic for the majority nucleoid population and were scored accordingly.

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