Comparative analysis of HIV-1 association with rhTRIM5α
| Viral envelope . | Cell type . | Treatment . | Total TRIM association . | TRIM association . | . | |
|---|---|---|---|---|---|---|
. | . | . | . | S15+ (unfused) . | S15− (fused) . | |
| % | % | % | ||||
| VSV-G | rhTRIM-HA HeLa | MG132 | 35.1 (Fig. 1 B) | ND | ND | |
| None | rhTRIM-HA HeLa | MG132 | 2.5 (Fig. 1 B) | ND | ND | |
| HIV | rhTRIM-HA HeLa | MG132 | 12.4 (Fig. 1 C) | ND | ND | |
| HIV | rhTRIM-HA HeLa | MG132 + AMD3100 | 7.3 (Fig. 1 C) | ND | ND | |
| VSV-G | rhTRIM-HA HeLa | BafA | 1.7a | 1.5 (Fig. 1 D) | 5.1 (Fig. 1 D) | |
| VSV-G | rhTRIM-HA HeLa | MG132 | 28.6a | 5.0 (Fig. 1 D) | 44.7 (Fig. 1 D) | |
| VSV-G | rhTRIM-HA CRFK | No drug | 17.8a | 4.7 (Fig. 3 B) | 32.1 (Fig. 3 B) | |
| VSV-G | rhΔpatch-HA CRFK | No drug | 5.1a | 3.2 (Fig. 3 B) | 7.5 (Fig. 3 B) | |
| VSV-G | rhTRIM-HA CRFK | MG132 | 13.7a | 5.4 (Fig. 3 C) | 26.5 (Fig. 3 C) | |
| VSV-G | rhΔpatch-HA CRFK | MG132 | 6.9a | 6.2 (Fig. 3 C) | 7.9 (Fig. 3 C) | |
| Viral envelope . | Cell type . | Treatment . | Total TRIM association . | TRIM association . | . | |
|---|---|---|---|---|---|---|
. | . | . | . | S15+ (unfused) . | S15− (fused) . | |
| % | % | % | ||||
| VSV-G | rhTRIM-HA HeLa | MG132 | 35.1 (Fig. 1 B) | ND | ND | |
| None | rhTRIM-HA HeLa | MG132 | 2.5 (Fig. 1 B) | ND | ND | |
| HIV | rhTRIM-HA HeLa | MG132 | 12.4 (Fig. 1 C) | ND | ND | |
| HIV | rhTRIM-HA HeLa | MG132 + AMD3100 | 7.3 (Fig. 1 C) | ND | ND | |
| VSV-G | rhTRIM-HA HeLa | BafA | 1.7a | 1.5 (Fig. 1 D) | 5.1 (Fig. 1 D) | |
| VSV-G | rhTRIM-HA HeLa | MG132 | 28.6a | 5.0 (Fig. 1 D) | 44.7 (Fig. 1 D) | |
| VSV-G | rhTRIM-HA CRFK | No drug | 17.8a | 4.7 (Fig. 3 B) | 32.1 (Fig. 3 B) | |
| VSV-G | rhΔpatch-HA CRFK | No drug | 5.1a | 3.2 (Fig. 3 B) | 7.5 (Fig. 3 B) | |
| VSV-G | rhTRIM-HA CRFK | MG132 | 13.7a | 5.4 (Fig. 3 C) | 26.5 (Fig. 3 C) | |
| VSV-G | rhΔpatch-HA CRFK | MG132 | 6.9a | 6.2 (Fig. 3 C) | 7.9 (Fig. 3 C) | |
Data from the experiments averaged in Figs. 1 and Figs.3 were pooled to examine the percentage of all viral particles associating with rhTRIM5α under all conditions examined. The figure from which the pooled data were derived is indicated in parentheses.
In the case of experiments examining S15-mCherry, data were pooled to determine the degree of rhTRIM5α associations in both S15+ and S15− populations.