Measured Parameters of S. pombe Interphase MT Dynamics
| . | . | MTs, cells . |
|---|---|---|
| . | . | n . |
| MT growth rate Vg | ||
| High GFP-tubulin expression level | 1.86 ± 0.54 μm min−1 | 67, 26 |
| Low GFP-tubulin expression level | 1.90 ± 0.33 μm min−1 | 7, 4 |
| Before tip contact | 2.08 ± 0.53 μm min−1 | 17, 10 |
| After tip contact | 1.30 ± 0.44 μm min−1 | 17, 10 |
| Average maximum ratio | 1.35 ± 0.33 | 31, 23 |
| MT shrinkage rate Vs | ||
| High GFP-tubulin expression level | 8.99 ± 2.82 μm min−1 | 64, 26 |
| Low GFP-tubulin expression level | 9.50 ± 3.85 μm min−1 | 7, 4 |
| MT catastrophe frequency fcat | 0.31 min−1 | 49, 19 |
| MT rescue frequency fres | 0 min−1 | 49, 19 |
| Duration of MT cell tip contact before catastrophe | 1.50 ± 0.59 min | 179, 33 |
| . | . | MTs, cells . |
|---|---|---|
| . | . | n . |
| MT growth rate Vg | ||
| High GFP-tubulin expression level | 1.86 ± 0.54 μm min−1 | 67, 26 |
| Low GFP-tubulin expression level | 1.90 ± 0.33 μm min−1 | 7, 4 |
| Before tip contact | 2.08 ± 0.53 μm min−1 | 17, 10 |
| After tip contact | 1.30 ± 0.44 μm min−1 | 17, 10 |
| Average maximum ratio | 1.35 ± 0.33 | 31, 23 |
| MT shrinkage rate Vs | ||
| High GFP-tubulin expression level | 8.99 ± 2.82 μm min−1 | 64, 26 |
| Low GFP-tubulin expression level | 9.50 ± 3.85 μm min−1 | 7, 4 |
| MT catastrophe frequency fcat | 0.31 min−1 | 49, 19 |
| MT rescue frequency fres | 0 min−1 | 49, 19 |
| Duration of MT cell tip contact before catastrophe | 1.50 ± 0.59 min | 179, 33 |
Rates were determined by linear regression analysis of each MT analyzed over 5–20 displacements measured 2–5 s apart. Data are given as mean ± SD of the rates measured for each MT, with n = number of MTs and cells measured.