Effect of Src activation on redistribution of Golgi resident proteins
| Golgi proteins tested | Redistributed to ER under EGF stimulation (HeLa cells) | Redistributed to ER under Src microinjection (either mouse SYF or human WI38 fibroblasts) |
| Structural proteins | ||
| Giantin | − | NT |
| Glycosyltransferases | ||
| Mannosidase II | − | − (SYF) |
| Mannosidase ICa | NT | − (SYF) |
| β4-GALTb | NT | − (SYF) |
| C2GnTc | NT | − (SYF) |
| GalNac-T1 | ++ | ++ (WI38) |
| GalNac-T2 | ++ | ++ (WI38) |
| GalNac-T3 | ++ | ++ (WI38) |
| GalNac-T4 | NT | ++ (WI38) |
| GalNac-T6 | NT | ++ (WI38) |
| COP-I regulatory proteins | ||
| Arf1d | ++ | ++ (SYF) |
| COP-I β1 | ++ | ++ (SYF) |
| COP-I γ1 | ++ | NT |
| Golgi proteins tested | Redistributed to ER under EGF stimulation (HeLa cells) | Redistributed to ER under Src microinjection (either mouse SYF or human WI38 fibroblasts) |
| Structural proteins | ||
| Giantin | − | NT |
| Glycosyltransferases | ||
| Mannosidase II | − | − (SYF) |
| Mannosidase ICa | NT | − (SYF) |
| β4-GALTb | NT | − (SYF) |
| C2GnTc | NT | − (SYF) |
| GalNac-T1 | ++ | ++ (WI38) |
| GalNac-T2 | ++ | ++ (WI38) |
| GalNac-T3 | ++ | ++ (WI38) |
| GalNac-T4 | NT | ++ (WI38) |
| GalNac-T6 | NT | ++ (WI38) |
| COP-I regulatory proteins | ||
| Arf1d | ++ | ++ (SYF) |
| COP-I β1 | ++ | ++ (SYF) |
| COP-I γ1 | ++ | NT |
−, not affected; ++, strong redistribution; NT, not tested.
Transfection of full-length Mannosidase IC tagged at its N terminus with an HA tag.
Transfection of β4-GALT GFP-tagged reporter construct.
Transfection of full-length C2GnT tagged at its C terminus with a DsRed tag.
Transfection of full-length Arf1 tagged at its C terminus with a GFP or a V5 tag.