Table I.

EM of ring formation on microtubules at different Dam1 complex concentrations

Dam1 complex concentration No. of microtubules Total microtubule length No. of rings Ring density 
nM  µm  µm−1a 
0.5 27 259 
16 280 20 0.1 ± 0.02 
26 308 76 0.2 ± 0.03 
26 256 450 2 ± 0.08 
10 18 33 535 17 ± 0.7 
50 13 16 1,312b 82 ± 3 
100 11 11 1,282b 120 ± 3 
200 11.5 1,358b 120 ± 3 
Dam1 complex concentration No. of microtubules Total microtubule length No. of rings Ring density 
nM  µm  µm−1a 
0.5 27 259 
16 280 20 0.1 ± 0.02 
26 308 76 0.2 ± 0.03 
26 256 450 2 ± 0.08 
10 18 33 535 17 ± 0.7 
50 13 16 1,312b 82 ± 3 
100 11 11 1,282b 120 ± 3 
200 11.5 1,358b 120 ± 3 
a

Errors represent counting uncertainties.

b

Rings stacked together in pairs to form doublets and/or coils.

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