Table I.

Yeast strains used in this study

Strain
Genotype
Source
972h− h− Yaffe laboratory 
FY254 h− ade6-M210 ura4-D18 leu1-32 can1-1 S. Forsburg 
FY261 h+ ade6-M216 ura4-D18 leu1-32 can1-1 S. Forsburg 
JW1084 h− ade6-M210 ura4-D18 leu1-32 sad1∷mRFP1-KanR J.Q. Wu 
ARC1687 h− ura4-D18 Δtea1∷ura4+ P. Nursea 
ARC3176 h− ade6- ura4-D18 leu1-32 his3-D1 Δtea2∷his3+ P. Nursea 
Δtip1 h+ ura4-D18 Δtip1∷KanR P. Nursea 
AY1491-1B h− ade6-210 lys1 ura4 Δdhc1-d4∷ura4+ Yamamoto et al., 1999  
DHC105 h+ his2 ura4 Δdhc1-d2∷ura4+ Yamamoto et al., 1999  
OM1602 h− ura4-294 P. Russellb 
UFY135 h+ ade6-M210 ura4-D18 leu1-32 his3Δ Δmal3∷his3+ U. Fleigc 
UFY596 h− ade6-M210 ura4-D18 leu1-32 his3D1 mal3-pkGFP:ura4+ U. Fleigc 
IH3505 h− ura4-D18 leu1-32 peg1-1 Grallert et al., 2006  
641 h− ade6-M210 ura4-D18 leu1-32 his3-D1 ars1∷nmt-atb1GFP:LEU2 R. McIntoshd 
MYP100 h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ Yaffe et al., 2003  
MYP115 h− ade6-M210 ura4-D18 leu1-32∷nmt1∷COX4GFP:leu1+ Weir and Yaffe, 2004  
MYP116 h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4GFP:leu1+ Yaffe laboratory 
MYP154 h− ade6-M210 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ Yaffe laboratory 
MYP160 h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ nmt1∷atb1GFP:LEU2 Yaffe laboratory 
MYP179 h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4GFP:leu1+ peg1-M4 EMS mutant of MYP116 
SCP119-1C h− ade6-M210 ura4-D18 leu1-32 can1-1 peg1-M4 Spore from MYP179/FY254 
SCP125-4A h+ ade6-M216 leu1-32∷nmt1∷COX4RFP:leu1+ Spore from MYP100/972h− 
SCP126-6A h+ ade6-M216 ura4-294 leu1-32∷nmt1∷COX4RFP:leu1+ Spore from SCP125-4A/OM1602 
SCP127 h+ ade6-M216 ura4-294 leu1-32∷nmt1∷COX4RFP:leu1+ h− ade6-M210 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ Diploid from SCP126-6A/MYP154 
SCP128-2C h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ nmt1∷atb1GFP:LEU2 peg1-M4 Spore from SCP119-1C/MYP160 
SCP130-1D h+ ade6-M216 ura4-294 leu1-32∷nmt1∷COX4GFP:leu1+ can1-1 peg1-M4 Spore from MYP179/OM1602 
SCP147 h+ ade6-M216 ura4-294 leu1-32∷nmt1∷COX4RFP:leu1+ Δpeg1∷KanR h− ade6-M210 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ Transformant of SCP127 
SCP164 h? ade6-M21? ura4-294∷pSC144(peg1-13myc):ura4 leu1-32∷nmt1∷COX4RFP:leu1+     Δpeg1∷KanR Spore of SCP147 + pSC144 
SCP177-2C h? ade6? Lys1? ura4-D18 leu1-32 Δdhc1-d4∷ura4+ peg1-M4 Spore from MYP179/AY1491-1B 
SCP178-1A h? ura4-D18 leu1-32 Δdhc1-d2∷ura4+ peg1-1 Spore from IH3505/DHC105 
SCP181-3A h? ade6? ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ sad1∷mRFP1-KanR peg1-M4 Spore from MYP179/JW1084 
SCP181-3D h? ade6? ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ sad1∷mRFP1-KanR Spore from MYP179/JW1084 
Strain
Genotype
Source
972h− h− Yaffe laboratory 
FY254 h− ade6-M210 ura4-D18 leu1-32 can1-1 S. Forsburg 
FY261 h+ ade6-M216 ura4-D18 leu1-32 can1-1 S. Forsburg 
JW1084 h− ade6-M210 ura4-D18 leu1-32 sad1∷mRFP1-KanR J.Q. Wu 
ARC1687 h− ura4-D18 Δtea1∷ura4+ P. Nursea 
ARC3176 h− ade6- ura4-D18 leu1-32 his3-D1 Δtea2∷his3+ P. Nursea 
Δtip1 h+ ura4-D18 Δtip1∷KanR P. Nursea 
AY1491-1B h− ade6-210 lys1 ura4 Δdhc1-d4∷ura4+ Yamamoto et al., 1999  
DHC105 h+ his2 ura4 Δdhc1-d2∷ura4+ Yamamoto et al., 1999  
OM1602 h− ura4-294 P. Russellb 
UFY135 h+ ade6-M210 ura4-D18 leu1-32 his3Δ Δmal3∷his3+ U. Fleigc 
UFY596 h− ade6-M210 ura4-D18 leu1-32 his3D1 mal3-pkGFP:ura4+ U. Fleigc 
IH3505 h− ura4-D18 leu1-32 peg1-1 Grallert et al., 2006  
641 h− ade6-M210 ura4-D18 leu1-32 his3-D1 ars1∷nmt-atb1GFP:LEU2 R. McIntoshd 
MYP100 h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ Yaffe et al., 2003  
MYP115 h− ade6-M210 ura4-D18 leu1-32∷nmt1∷COX4GFP:leu1+ Weir and Yaffe, 2004  
MYP116 h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4GFP:leu1+ Yaffe laboratory 
MYP154 h− ade6-M210 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ Yaffe laboratory 
MYP160 h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ nmt1∷atb1GFP:LEU2 Yaffe laboratory 
MYP179 h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4GFP:leu1+ peg1-M4 EMS mutant of MYP116 
SCP119-1C h− ade6-M210 ura4-D18 leu1-32 can1-1 peg1-M4 Spore from MYP179/FY254 
SCP125-4A h+ ade6-M216 leu1-32∷nmt1∷COX4RFP:leu1+ Spore from MYP100/972h− 
SCP126-6A h+ ade6-M216 ura4-294 leu1-32∷nmt1∷COX4RFP:leu1+ Spore from SCP125-4A/OM1602 
SCP127 h+ ade6-M216 ura4-294 leu1-32∷nmt1∷COX4RFP:leu1+ h− ade6-M210 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ Diploid from SCP126-6A/MYP154 
SCP128-2C h+ ade6-M216 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ nmt1∷atb1GFP:LEU2 peg1-M4 Spore from SCP119-1C/MYP160 
SCP130-1D h+ ade6-M216 ura4-294 leu1-32∷nmt1∷COX4GFP:leu1+ can1-1 peg1-M4 Spore from MYP179/OM1602 
SCP147 h+ ade6-M216 ura4-294 leu1-32∷nmt1∷COX4RFP:leu1+ Δpeg1∷KanR h− ade6-M210 ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ Transformant of SCP127 
SCP164 h? ade6-M21? ura4-294∷pSC144(peg1-13myc):ura4 leu1-32∷nmt1∷COX4RFP:leu1+     Δpeg1∷KanR Spore of SCP147 + pSC144 
SCP177-2C h? ade6? Lys1? ura4-D18 leu1-32 Δdhc1-d4∷ura4+ peg1-M4 Spore from MYP179/AY1491-1B 
SCP178-1A h? ura4-D18 leu1-32 Δdhc1-d2∷ura4+ peg1-1 Spore from IH3505/DHC105 
SCP181-3A h? ade6? ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ sad1∷mRFP1-KanR peg1-M4 Spore from MYP179/JW1084 
SCP181-3D h? ade6? ura4-D18 leu1-32∷nmt1∷COX4RFP:leu1+ sad1∷mRFP1-KanR Spore from MYP179/JW1084 
a

The Rockefeller University, New York, NY.

b

The Scripps Research Institute, La Jolla, CA.

c

Heinrich-Heine Universität Düsseldorf, Düsseldorf, Germany.

d

University of Colorado, Boulder, CO.

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