Current activation properties of CaV1.1a-D1223A-D1225A, γ1-R160A, γ1-K102A-E103A, and γ1-R160A-K102A-E103A (RKE AAA) mutants
| Property . | CaV1.1a . | CaV1.1a + γ1 . | P value (ANOVA) . | CaV1.1a + γ1-R160 . | P value (ANOVA) . |
|---|---|---|---|---|---|
| Ipeak (pA/pF) | −20.1 ± 3.4 | −10.1 ± 1.9 | 0.04* | −13.3 ± 2.3 | 0.20 |
| V1/2 activation (mV) | 23.1 ± 0.6 | 30.7 ± 2.2 | 0.004** | 24.1 ± 1.3 | 0.88 |
| k activation (mV) | 7.0 ± 0.3 | 13.1 ± 1.8 | 0.001** | 8.5 ± 0.4 | 0.58 |
| Vrev (mV) | 82.7 ± 2.2 | 86.1 ± 3.9 | 0.65 | 87.4 ± 1.3 | 0.42 |
| Time to peak (ms) | 131.8 ± 18.8 | 102.6 ± 8.4 | 0.26 | 95.3 ± 8.9 | 0.12 |
| n | 10 | 10 | 11 | ||
| CaV1.1a D1223A–1225A | CaV1.1a D1223A–1225A + γ1 | P value (t test) | |||
| Ipeak (pA/pF) | −26.1 ± 6.6 | −10.4 ± 2.6 | 0.04* | ||
| V1/2 activation (mV) | 12.8 ± 0.8 | 15.8 ± 0.9 | 0.02* | ||
| k activation (mV) | 6.3 ± 0.4 | 7.1 ± 0.5 | 0.22 | ||
| Vrev (mV) | 73.5 ± 2.5 | 69.7 ± 4.3 | 0.44 | ||
| Time to peak (ms) | 87.2 ± 11.0 | 66.6 ± 10.7 | 0.20 | ||
| n | 14 | 13 | |||
| CaV1.1a | CaV1.1a + γ1 | P value (ANOVA) | CaV1.1a + γ1-K103A-E104A | P value (ANOVA) | |
| Ipeak (pA/pF) | −23.6 ± 4.2 | −13.9 ± 3.1 | 0.13 | −14.0 ± 2.1 | 0.09 |
| V1/2 activation (mV) | 25.1 ± 1.0 | 26.9 ± 0.9 | 0.40 | 28.2 ± 0.9 | 0.06 |
| k activation (mV) | 8.7 ± 0.5 | 9.8 ± 0.5 | 0.20 | 8.7 ± 0.4 | 0.99 |
| Vrev (mV) | 86.7 ± 1.8 | 91.8 ± 2.0 | 0.24 | 81.2 ± 2.7 | 0.17 |
| Time to peak (ms) | 121.5 ± 18.5 | 99.8 ± 23.1 | 0.69 | 108.5 ± 13.3 | 0.85 |
| n | 10 | 8 | – | 11 | |
| CaV1.1a | CaV1.1a + γ1 | P value (ANOVA) | CaV1.1a + γ1-RKE AAA | P value (ANOVA) | |
| Ipeak (pA/pF) | −12.3 ± 1.5 | −6.6 ± 0.8 | 0.006** | −6.6 ± 1.2 | 0.008** |
| V1/2 activation (mV) | 20.6 ± 0.9 | 28.2 ± 3.3 | 0.13 | 25.7 ± 4.2 | 0.38 |
| k activation (mV) | 8.9 ± 0.6 | 13.9 ± 2.2 | 0.07 | 13.4 ± 2.0 | 0.12 |
| Vrev (mV) | 89.8 ± 2.0 | 83.2 ± 4.2 | 0.24 | 92.5 ± 2.9 | 0.76 |
| Time to peak (ms) | |||||
| n | 12 | 11 | 10 |
| Property . | CaV1.1a . | CaV1.1a + γ1 . | P value (ANOVA) . | CaV1.1a + γ1-R160 . | P value (ANOVA) . |
|---|---|---|---|---|---|
| Ipeak (pA/pF) | −20.1 ± 3.4 | −10.1 ± 1.9 | 0.04* | −13.3 ± 2.3 | 0.20 |
| V1/2 activation (mV) | 23.1 ± 0.6 | 30.7 ± 2.2 | 0.004** | 24.1 ± 1.3 | 0.88 |
| k activation (mV) | 7.0 ± 0.3 | 13.1 ± 1.8 | 0.001** | 8.5 ± 0.4 | 0.58 |
| Vrev (mV) | 82.7 ± 2.2 | 86.1 ± 3.9 | 0.65 | 87.4 ± 1.3 | 0.42 |
| Time to peak (ms) | 131.8 ± 18.8 | 102.6 ± 8.4 | 0.26 | 95.3 ± 8.9 | 0.12 |
| n | 10 | 10 | 11 | ||
| CaV1.1a D1223A–1225A | CaV1.1a D1223A–1225A + γ1 | P value (t test) | |||
| Ipeak (pA/pF) | −26.1 ± 6.6 | −10.4 ± 2.6 | 0.04* | ||
| V1/2 activation (mV) | 12.8 ± 0.8 | 15.8 ± 0.9 | 0.02* | ||
| k activation (mV) | 6.3 ± 0.4 | 7.1 ± 0.5 | 0.22 | ||
| Vrev (mV) | 73.5 ± 2.5 | 69.7 ± 4.3 | 0.44 | ||
| Time to peak (ms) | 87.2 ± 11.0 | 66.6 ± 10.7 | 0.20 | ||
| n | 14 | 13 | |||
| CaV1.1a | CaV1.1a + γ1 | P value (ANOVA) | CaV1.1a + γ1-K103A-E104A | P value (ANOVA) | |
| Ipeak (pA/pF) | −23.6 ± 4.2 | −13.9 ± 3.1 | 0.13 | −14.0 ± 2.1 | 0.09 |
| V1/2 activation (mV) | 25.1 ± 1.0 | 26.9 ± 0.9 | 0.40 | 28.2 ± 0.9 | 0.06 |
| k activation (mV) | 8.7 ± 0.5 | 9.8 ± 0.5 | 0.20 | 8.7 ± 0.4 | 0.99 |
| Vrev (mV) | 86.7 ± 1.8 | 91.8 ± 2.0 | 0.24 | 81.2 ± 2.7 | 0.17 |
| Time to peak (ms) | 121.5 ± 18.5 | 99.8 ± 23.1 | 0.69 | 108.5 ± 13.3 | 0.85 |
| n | 10 | 8 | – | 11 | |
| CaV1.1a | CaV1.1a + γ1 | P value (ANOVA) | CaV1.1a + γ1-RKE AAA | P value (ANOVA) | |
| Ipeak (pA/pF) | −12.3 ± 1.5 | −6.6 ± 0.8 | 0.006** | −6.6 ± 1.2 | 0.008** |
| V1/2 activation (mV) | 20.6 ± 0.9 | 28.2 ± 3.3 | 0.13 | 25.7 ± 4.2 | 0.38 |
| k activation (mV) | 8.9 ± 0.6 | 13.9 ± 2.2 | 0.07 | 13.4 ± 2.0 | 0.12 |
| Vrev (mV) | 89.8 ± 2.0 | 83.2 ± 4.2 | 0.24 | 92.5 ± 2.9 | 0.76 |
| Time to peak (ms) | |||||
| n | 12 | 11 | 10 |
Data are expressed as mean values ± SEM. Statistically significant: *, P < 0.05; **, P < 0.01.