Table 1.
Probe sets for smRNA FISH
Sequence (5′–3′)
Hsp70 for CHO Hsp70 MSH5 VARS LSM C6orf48 MS2 repeats
CAC​GCA​CGA​GTA​GGT​GGT​G  GGG​AGT​CAC​TCT​CGA​AAG​AC  CCA​CGA​GCC​TGC​AAA​AGG​A  ACACCCCTGAGCACGACG  GAG​CGC​AAG​CTG​GGT​AGA​G  GAG​CCC​ACT​TCG​CAA​AAA​G  CAT​GTT​TTC​TAG​AGT​CGA​CC  
CTTCCGTGCTGGAACACG  CAC​AGG​TTC​GCT​CTG​GAA​AG  CGC​TAC​AGG​TGG​GAG​AAC​G  CGCGGACGCAGGACGAGA  CAA​GCG​CTG​ACG​GGC​AAA​G  AAC​TCT​CAT​ACT​GCC​AAC​C  ATA​CTG​CAG​ACA​TGG​GTG​AT  
TGG​TCG​TTG​GCG​ATG​ATC​T  AAC​GCC​GGA​AAC​TCA​ACA​CG  CGC​CTT​TTC​AGT​AAC​CTG​A  CCGGTCTCACGAGGAACA  GCG​GGA​AGC​GAC​GCA​GAA​A  CAC​TCA​ACA​GTC​GGG​CCA​T  CCT​TAG​GAT​CTA​ATG​AAC​CC  
GTC​GGT​GAA​GGC​CAC​GTA​G  CGA​CAA​GAG​CTC​AGT​CCT​T  TCA​CGC​GCT​TAT​CTT​CCT​C  CTTTGTGACAGGGAGCGT  CAG​GTC​TGG​GGA​AAC​CGA​A  TGC​GCA​AAG​GCA​GCG​CAA​G  TGA​TCC​TCA​TGT​TTT​CTA​GG  
CGT​CGA​AAC​ACG​GTG​TTC​TG  TGA​GAC​TGG​GGG​CTG​GAA​AC  GAG​TCG​TGC​ACG​TCT​TAT​G  TAGTTCCCTAAGATCGCC  GGA​AGA​CAG​CAG​GGT​GCT​G  CAA​CGG​TAG​TTC​ACC​CAA​C  AGA​GTC​GAC​CTG​CAG​ACA​TG  
CGA​ACT​TGC​GGC​CGA​TCA​G  TGG​TCG​TTG​GCG​ATG​ATC​TC  GAA​GGA​AGG​GGT​CTG​AGG​G  AGTCGAGCGGGCAGAGAC  CTT​TTG​ACG​TCA​CGG​TAC​C  AGA​CAC​CAG​AAA​CTC​CAG​T  ATG​GGT​GAT​CCT​CAT​GTT​TT  
GAT​CTC​CTC​GGG​GTA​GAA​G  TTC​GCG​TCA​AAC​ACG​GTG​TT  TCA​TTC​CTG​TCA​CGC​GGA​G  AATCCACCTCACAGCCAG  GGT​ACA​AAG​GCC​AGA​TCC​C  GAG​CTA​GGT​CAG​TTC​CAA​G  AAT​GAA​CCC​GGG​AAT​ACT​GC  
CAT​CTT​CGT​CAG​CAC​CAT​G  TTG​TCT​CCG​TCG​TTG​ATC​AC  ATT​GTG​GGA​AAC​TCC​ACG​C  AGATGGTCAGACTGGGCC  CTC​CTC​AAT​GAA​CCT​GAG​A  TTG​TTT​TGA​GTC​AGC​AGG​G  AGG​CAA​TTA​GGT​ACC​TTA​GG  
TGA​TCA​CCG​CGT​TGG​TCA​C  AGA​TCT​CCT​CGG​GGT​AGA​AT  GGT​GAA​TTC​TCG​GGT​ATT​T  CGCGTGTACGTACTGGAG  ATA​TCC​CAT​TTG​TTC​TCA​G  CCA​TTT​TGC​AAT​CAC​TCG​C   
GAG​TCG​TTG​AAG​TAG​GCG​G  ATC​TCC​TTC​ATC​TTG​GTC​AG  TGG​AGA​GCT​GTG​GAC​ACA​G  TCGGCGTCTGGTTGGATG  TTT​TTT​CCC​TCA​TCA​TGG​A  ATC​ATC​ACT​CCC​TTC​CTA​T   
CGT​GGG​CTC​GTT​GAT​GAT​C  CTG​CGA​GTC​GTT​GAA​GTA​GG  TAC​TTC​TGC​TAC​AGG​GCT​G  GGAGTGTGGAAGGCTCTC  TCC​TCA​CTG​AAT​CTC​TCT​C  CAC​TCA​AGA​GTT​ACC​TGG​G   
CCA​GGT​CGA​AGA​TGA​GCA​C  ATG​ATC​CGC​AGC​ACG​TTG​AG  TGG​CGC​GCA​GAA​TGC​AAA​G  AACTATCCACCATCGCGG  AGG​TCT​AAC​TTT​TCC​GTC​T  TCA​GAG​CGC​TGC​GGT​GAT​G   
ATG​GAC​ACG​TCG​AAC​GTG​C  TCA​GGA​TGG​ACA​CGT​CGA​AG  ACG​ATT​CAC​AGA​GGA​GGC​C  GGGGAGTTCCTGGGGAAG  AAG​CTT​GCC​TGG​CAG​AGA​A  AAA​TGC​TGG​ACC​GAG​GGG​G   
GAA​GAT​GCC​GTC​GTC​GAT​C  TCG​AAG​ATG​CCG​TCG​TCG​AT  AAA​AGT​GAG​GGC​GGT​TCG​G  TCTCAGAGGGGCAGTGTC  AGA​AAA​TAT​CCC​ACC​CGC​A  CCA​TGA​ACT​CGT​TGA​GCC​T   
CAC​GAA​GTG​GCT​CAC​CAG​C  CCC​TCA​AAC​AGG​GAG​TCG​AT  CAT​GAG​CTT​GGA​GGC​TCT​G  CTGTCAGGAGCCGAGGAC  CCC​ACT​CCT​TTC​AAT​GAA​T  TTA​CAA​CTC​CTA​ACG​GGG​A   
TGG​ACG​ACA​GGG​TCC​TCT​T  GGT​GAT​GGA​CGT​GTA​GAA​GT  CTTGGGTTCGCTCCTAAG  CTGAGGGTCTGACCAGGC  CTG​TGC​TCT​CTC​AGT​CGA​C  GGA​TAA​CAC​GGC​GAT​GAA​C   
CCT​CGA​ACA​GGG​AGT​CGA​T  TTC​GGA​ACA​GGT​CGG​AGC​AC  CTGGGGAAGCCGGAGGAG  AGACACCCGAGTCCCATA  AAG​TCC​CAG​AGA​GAC​TCT​G  CCT​AAT​ACT​CAC​CTT​TAC​T   
CGC​CGT​GAT​GGA​CGT​GTA​GA  AGG​ACC​AGG​TCG​TGA​ATC​TG  TTC​TAC​TCC​CCT​CAG​AGA​C  CTCACGGGAGCTCCTTCG  AAG​GCT​GGA​GCC​CAA​ATT​A  AGG​CAT​TCA​AAA​GGC​TCT​C   
GGA​ACA​GGT​CCG​AGC​ACA​G  TTG​AAG​AAG​TCC​TGC​AGC​AG  CCA​TCA​ACT​CTC​CAT​TCA​A  GGTCCTATGTTTGAGTAG  CAT​CCC​TGA​CAG​TTC​TCA​A  TTG​GGA​ACA​AAG​CTT​TCC​G   
CTT​CTG​CAC​CTT​GGG​GAT​G  TTG​ATG​CTC​TTG​TTC​AGG​TC  CCA​ACC​CTC​TTT​TAT​TCT​A  GAAGACTGCGGGATCGAG  TAC​TGG​TAT​TGT​GAA​CCC​C  ATG​CCC​ATG​AAC​GAA​AGC​T   
GTT​GAA​GAA​GTC​CTG​CAG​C  TGC​ACG​TTC​TCG​GAC​TTG​TC  GAT​CTG​TCC​AGC​AAG​GAA​G  AGGTCCGAACGAAGTGGA  GGA​GTG​TCC​TTT​GAC​AGT​A  ACT​CCT​ATT​TTG​CAG​TAG​A   
GAT​GCT​CTT​GTT​GAG​GTC​G  TTG​ATC​AGG​GCA​GTC​ATC​AC  ATT​CCA​CAG​CAC​ACA​CAG​A  GGGAGACGTAGAGGGTGG  TGT​GGG​AAG​GAG​CAT​GGT​A  GGA​CGT​TAG​AAA​GGG​AGG​A   
CTG​CAC​GTT​CTC​AGA​CTT​G  TCG​TAC​ACC​TGG​ATC​AGC​AC  TAG​GCA​ATG​CCC​AAG​TAT​C  CTGGGGAAGGCATCTGGG  GGG​AGA​GAG​GGG​GAA​AAC​C  AGG​GAA​GCT​CTT​CTG​GAA​A   
TTG​ATG​AGC​GCC​GTC​ATC​A  CAG​ATT​GTT​GTC​TTT​CGT​CA  GTG​GAG​TCA​CTA​GTA​TCA​T  TAGCGAGCGGCTATGAGG  TAG​TTC​CAC​GAC​CAC​ATC​C  CCA​ATT​AGG​GAG​ATC​TGG​A   
GAG​TAG​GTG​GTG​AAG​GTC​T  ATG​TCG​AAG​GTC​ACC​TCG​AT  GCA​TCT​GGC​ATG​AAG​TGG​A  GTGGCTGGAGACAGATGC  GTA​CCT​ACC​TCA​GGT​CAT​T  AGC​ATC​GGA​GAC​TCT​AGT​C   
CGT​ACA​CCT​GGA​TCA​GCA​C  TGA​CGT​TCA​GGA​TGC​CGT​TG  GAG​AAG​CTT​GAG​GCT​CTC​G  GTTGGACAAGGACAGCCG  CCC​AAC​AGA​CTT​GTT​GGA​A  GTT​CCC​TAA​ATG​AGT​CAG​A   
GAT​GCC​GCT​GAG​CTC​GAA​G  TCA​TGT​TGA​AGG​CGT​AGG​AC  GGA​ATT​CAT​GGT​TCC​ATC​C  CGTGTCGGCGTAACTGAC  TGC​TTT​GTA​TTG​TTT​TCC​A  CTT​CTG​GAG​ACC​CAA​GTA​T   
ATC​GAT​GTC​GAA​GGT​CAC​C  TGT​CCA​GAA​CCT​TCT​TCT​TG  CAT​CTG​CAA​TCC​CAG​AGA​G  CACAGGCAGCTGGTATTA  AAAGAGGTTCCAGGGCCC  TGG​GCT​TCC​AGA​GTT​CAT​T   
TGA​CGT​TCA​GGA​TGC​CGT​T  CAC​GAG​ATG​ACC​TCT​TGA​CA   CGAGCTTCGGAGTCCCAG  GTACATCTTCCACCTCGC  CTC​TGT​GAA​GGT​GCA​TTG​T   
TAG​GAC​TCG​AGC​GCG​TTC​T  TTG​TGC​TCA​AAC​TCG​TCC​TT    AGG​CAC​AAG​GCA​TTT​ATT​T  CCT​TCA​CTC​AGA​TTA​GTG​C   
GCG​CTC​TTC​ATG​TTG​AAG​G  CTG​ATG​ATG​GGG​TTA​CAC​AC    ACT​GCG​CCT​GAC​CTG​TAT​T  GAG​GGG​GAG​ATT​CCA​AAC​C   
AGG​AGA​TGA​CCT​CCT​GAC​A  ACT​AAA​GAA​CAA​AGG​CCC​CT     GCC​ATA​CAA​AGC​TTC​TCT​C   
CAC​GAA​CTC​CTC​CTT​GTC​G  AAG​TCC​TTG​AGT​CCC​AAC​AG       
CTA​ATC​CAC​CTC​CTC​GAT​G  CCA​TCA​GGT​TAC​AAC​TTA​AC       
ACA​CCG​GGA​GAG​CAA​GCA​G        
AGG​GCT​AAC​TAA​CCC​TGA​C        
Sequence (5′–3′)
Hsp70 for CHO Hsp70 MSH5 VARS LSM C6orf48 MS2 repeats
CAC​GCA​CGA​GTA​GGT​GGT​G  GGG​AGT​CAC​TCT​CGA​AAG​AC  CCA​CGA​GCC​TGC​AAA​AGG​A  ACACCCCTGAGCACGACG  GAG​CGC​AAG​CTG​GGT​AGA​G  GAG​CCC​ACT​TCG​CAA​AAA​G  CAT​GTT​TTC​TAG​AGT​CGA​CC  
CTTCCGTGCTGGAACACG  CAC​AGG​TTC​GCT​CTG​GAA​AG  CGC​TAC​AGG​TGG​GAG​AAC​G  CGCGGACGCAGGACGAGA  CAA​GCG​CTG​ACG​GGC​AAA​G  AAC​TCT​CAT​ACT​GCC​AAC​C  ATA​CTG​CAG​ACA​TGG​GTG​AT  
TGG​TCG​TTG​GCG​ATG​ATC​T  AAC​GCC​GGA​AAC​TCA​ACA​CG  CGC​CTT​TTC​AGT​AAC​CTG​A  CCGGTCTCACGAGGAACA  GCG​GGA​AGC​GAC​GCA​GAA​A  CAC​TCA​ACA​GTC​GGG​CCA​T  CCT​TAG​GAT​CTA​ATG​AAC​CC  
GTC​GGT​GAA​GGC​CAC​GTA​G  CGA​CAA​GAG​CTC​AGT​CCT​T  TCA​CGC​GCT​TAT​CTT​CCT​C  CTTTGTGACAGGGAGCGT  CAG​GTC​TGG​GGA​AAC​CGA​A  TGC​GCA​AAG​GCA​GCG​CAA​G  TGA​TCC​TCA​TGT​TTT​CTA​GG  
CGT​CGA​AAC​ACG​GTG​TTC​TG  TGA​GAC​TGG​GGG​CTG​GAA​AC  GAG​TCG​TGC​ACG​TCT​TAT​G  TAGTTCCCTAAGATCGCC  GGA​AGA​CAG​CAG​GGT​GCT​G  CAA​CGG​TAG​TTC​ACC​CAA​C  AGA​GTC​GAC​CTG​CAG​ACA​TG  
CGA​ACT​TGC​GGC​CGA​TCA​G  TGG​TCG​TTG​GCG​ATG​ATC​TC  GAA​GGA​AGG​GGT​CTG​AGG​G  AGTCGAGCGGGCAGAGAC  CTT​TTG​ACG​TCA​CGG​TAC​C  AGA​CAC​CAG​AAA​CTC​CAG​T  ATG​GGT​GAT​CCT​CAT​GTT​TT  
GAT​CTC​CTC​GGG​GTA​GAA​G  TTC​GCG​TCA​AAC​ACG​GTG​TT  TCA​TTC​CTG​TCA​CGC​GGA​G  AATCCACCTCACAGCCAG  GGT​ACA​AAG​GCC​AGA​TCC​C  GAG​CTA​GGT​CAG​TTC​CAA​G  AAT​GAA​CCC​GGG​AAT​ACT​GC  
CAT​CTT​CGT​CAG​CAC​CAT​G  TTG​TCT​CCG​TCG​TTG​ATC​AC  ATT​GTG​GGA​AAC​TCC​ACG​C  AGATGGTCAGACTGGGCC  CTC​CTC​AAT​GAA​CCT​GAG​A  TTG​TTT​TGA​GTC​AGC​AGG​G  AGG​CAA​TTA​GGT​ACC​TTA​GG  
TGA​TCA​CCG​CGT​TGG​TCA​C  AGA​TCT​CCT​CGG​GGT​AGA​AT  GGT​GAA​TTC​TCG​GGT​ATT​T  CGCGTGTACGTACTGGAG  ATA​TCC​CAT​TTG​TTC​TCA​G  CCA​TTT​TGC​AAT​CAC​TCG​C   
GAG​TCG​TTG​AAG​TAG​GCG​G  ATC​TCC​TTC​ATC​TTG​GTC​AG  TGG​AGA​GCT​GTG​GAC​ACA​G  TCGGCGTCTGGTTGGATG  TTT​TTT​CCC​TCA​TCA​TGG​A  ATC​ATC​ACT​CCC​TTC​CTA​T   
CGT​GGG​CTC​GTT​GAT​GAT​C  CTG​CGA​GTC​GTT​GAA​GTA​GG  TAC​TTC​TGC​TAC​AGG​GCT​G  GGAGTGTGGAAGGCTCTC  TCC​TCA​CTG​AAT​CTC​TCT​C  CAC​TCA​AGA​GTT​ACC​TGG​G   
CCA​GGT​CGA​AGA​TGA​GCA​C  ATG​ATC​CGC​AGC​ACG​TTG​AG  TGG​CGC​GCA​GAA​TGC​AAA​G  AACTATCCACCATCGCGG  AGG​TCT​AAC​TTT​TCC​GTC​T  TCA​GAG​CGC​TGC​GGT​GAT​G   
ATG​GAC​ACG​TCG​AAC​GTG​C  TCA​GGA​TGG​ACA​CGT​CGA​AG  ACG​ATT​CAC​AGA​GGA​GGC​C  GGGGAGTTCCTGGGGAAG  AAG​CTT​GCC​TGG​CAG​AGA​A  AAA​TGC​TGG​ACC​GAG​GGG​G   
GAA​GAT​GCC​GTC​GTC​GAT​C  TCG​AAG​ATG​CCG​TCG​TCG​AT  AAA​AGT​GAG​GGC​GGT​TCG​G  TCTCAGAGGGGCAGTGTC  AGA​AAA​TAT​CCC​ACC​CGC​A  CCA​TGA​ACT​CGT​TGA​GCC​T   
CAC​GAA​GTG​GCT​CAC​CAG​C  CCC​TCA​AAC​AGG​GAG​TCG​AT  CAT​GAG​CTT​GGA​GGC​TCT​G  CTGTCAGGAGCCGAGGAC  CCC​ACT​CCT​TTC​AAT​GAA​T  TTA​CAA​CTC​CTA​ACG​GGG​A   
TGG​ACG​ACA​GGG​TCC​TCT​T  GGT​GAT​GGA​CGT​GTA​GAA​GT  CTTGGGTTCGCTCCTAAG  CTGAGGGTCTGACCAGGC  CTG​TGC​TCT​CTC​AGT​CGA​C  GGA​TAA​CAC​GGC​GAT​GAA​C   
CCT​CGA​ACA​GGG​AGT​CGA​T  TTC​GGA​ACA​GGT​CGG​AGC​AC  CTGGGGAAGCCGGAGGAG  AGACACCCGAGTCCCATA  AAG​TCC​CAG​AGA​GAC​TCT​G  CCT​AAT​ACT​CAC​CTT​TAC​T   
CGC​CGT​GAT​GGA​CGT​GTA​GA  AGG​ACC​AGG​TCG​TGA​ATC​TG  TTC​TAC​TCC​CCT​CAG​AGA​C  CTCACGGGAGCTCCTTCG  AAG​GCT​GGA​GCC​CAA​ATT​A  AGG​CAT​TCA​AAA​GGC​TCT​C   
GGA​ACA​GGT​CCG​AGC​ACA​G  TTG​AAG​AAG​TCC​TGC​AGC​AG  CCA​TCA​ACT​CTC​CAT​TCA​A  GGTCCTATGTTTGAGTAG  CAT​CCC​TGA​CAG​TTC​TCA​A  TTG​GGA​ACA​AAG​CTT​TCC​G   
CTT​CTG​CAC​CTT​GGG​GAT​G  TTG​ATG​CTC​TTG​TTC​AGG​TC  CCA​ACC​CTC​TTT​TAT​TCT​A  GAAGACTGCGGGATCGAG  TAC​TGG​TAT​TGT​GAA​CCC​C  ATG​CCC​ATG​AAC​GAA​AGC​T   
GTT​GAA​GAA​GTC​CTG​CAG​C  TGC​ACG​TTC​TCG​GAC​TTG​TC  GAT​CTG​TCC​AGC​AAG​GAA​G  AGGTCCGAACGAAGTGGA  GGA​GTG​TCC​TTT​GAC​AGT​A  ACT​CCT​ATT​TTG​CAG​TAG​A   
GAT​GCT​CTT​GTT​GAG​GTC​G  TTG​ATC​AGG​GCA​GTC​ATC​AC  ATT​CCA​CAG​CAC​ACA​CAG​A  GGGAGACGTAGAGGGTGG  TGT​GGG​AAG​GAG​CAT​GGT​A  GGA​CGT​TAG​AAA​GGG​AGG​A   
CTG​CAC​GTT​CTC​AGA​CTT​G  TCG​TAC​ACC​TGG​ATC​AGC​AC  TAG​GCA​ATG​CCC​AAG​TAT​C  CTGGGGAAGGCATCTGGG  GGG​AGA​GAG​GGG​GAA​AAC​C  AGG​GAA​GCT​CTT​CTG​GAA​A   
TTG​ATG​AGC​GCC​GTC​ATC​A  CAG​ATT​GTT​GTC​TTT​CGT​CA  GTG​GAG​TCA​CTA​GTA​TCA​T  TAGCGAGCGGCTATGAGG  TAG​TTC​CAC​GAC​CAC​ATC​C  CCA​ATT​AGG​GAG​ATC​TGG​A   
GAG​TAG​GTG​GTG​AAG​GTC​T  ATG​TCG​AAG​GTC​ACC​TCG​AT  GCA​TCT​GGC​ATG​AAG​TGG​A  GTGGCTGGAGACAGATGC  GTA​CCT​ACC​TCA​GGT​CAT​T  AGC​ATC​GGA​GAC​TCT​AGT​C   
CGT​ACA​CCT​GGA​TCA​GCA​C  TGA​CGT​TCA​GGA​TGC​CGT​TG  GAG​AAG​CTT​GAG​GCT​CTC​G  GTTGGACAAGGACAGCCG  CCC​AAC​AGA​CTT​GTT​GGA​A  GTT​CCC​TAA​ATG​AGT​CAG​A   
GAT​GCC​GCT​GAG​CTC​GAA​G  TCA​TGT​TGA​AGG​CGT​AGG​AC  GGA​ATT​CAT​GGT​TCC​ATC​C  CGTGTCGGCGTAACTGAC  TGC​TTT​GTA​TTG​TTT​TCC​A  CTT​CTG​GAG​ACC​CAA​GTA​T   
ATC​GAT​GTC​GAA​GGT​CAC​C  TGT​CCA​GAA​CCT​TCT​TCT​TG  CAT​CTG​CAA​TCC​CAG​AGA​G  CACAGGCAGCTGGTATTA  AAAGAGGTTCCAGGGCCC  TGG​GCT​TCC​AGA​GTT​CAT​T   
TGA​CGT​TCA​GGA​TGC​CGT​T  CAC​GAG​ATG​ACC​TCT​TGA​CA   CGAGCTTCGGAGTCCCAG  GTACATCTTCCACCTCGC  CTC​TGT​GAA​GGT​GCA​TTG​T   
TAG​GAC​TCG​AGC​GCG​TTC​T  TTG​TGC​TCA​AAC​TCG​TCC​TT    AGG​CAC​AAG​GCA​TTT​ATT​T  CCT​TCA​CTC​AGA​TTA​GTG​C   
GCG​CTC​TTC​ATG​TTG​AAG​G  CTG​ATG​ATG​GGG​TTA​CAC​AC    ACT​GCG​CCT​GAC​CTG​TAT​T  GAG​GGG​GAG​ATT​CCA​AAC​C   
AGG​AGA​TGA​CCT​CCT​GAC​A  ACT​AAA​GAA​CAA​AGG​CCC​CT     GCC​ATA​CAA​AGC​TTC​TCT​C   
CAC​GAA​CTC​CTC​CTT​GTC​G  AAG​TCC​TTG​AGT​CCC​AAC​AG       
CTA​ATC​CAC​CTC​CTC​GAT​G  CCA​TCA​GGT​TAC​AAC​TTA​AC       
ACA​CCG​GGA​GAG​CAA​GCA​G        
AGG​GCT​AAC​TAA​CCC​TGA​C        
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