Table 1.

Viruses recognized by ZBP1

VirusFamilyProposed antiviral function(s)Role(s) in in vivo mouse modelsViral ZBP1 antagonistZBP1 agonistReferences
MCMV Herpesviridae (large dsDNA) Necroptosis Restriction of viral replication
Protective 
M45 (vIRA) inhibits necroptosis RNA Jiao et al., 2020; Maelfait et al., 2017; Sridharan et al., 2017; Upton et al., 2012  
UV-inactivated human cytomegalovirus Contribution to type I IFN response n.a. UL36 potentially inhibits necroptosis n.d. DeFilippis et al., 2010  
HSV-1 Necroptosis and apoptosis (and pyroptosis in BMDMs)
Contribution to type I IFN response and inflammatory cytokine production
Potential inhibition of ICP0-mediated degradation of IFI16 
Restriction of viral replication ICP6 (UL39) inhibits necroptosis n.d. Furr et al., 2011; Guo et al., 2018; Jeffries et al., 2022; Lee et al., 2021; Pham et al., 2013; Takaoka et al., 2007  
HSV-2 Necroptosis?
Contribution to type I IFN response 
n.d. ICP10 (UL39) potentially inhibits necroptosis n.d. Triantafilou et al., 2014  
VZV Apoptosis n.d. Triplex capsid subunit 1 (ORF20) inhibits caspase-dependent cell death n.d. Steain et al., 2020  
Vaccinia virus Poxviridae (large dsDNA) Necroptosis Restriction of viral replication
Protective 
E3 (E3L) sequesters Z-RNA Z-RNA Koehler et al., 2017, 2021  
IAV Orthomyxoviridae (−ssRNA) Apoptosis and necroptosis (and pyroptosis in BMDMs)
Contribution to inflammatory cytokine production 
Restriction of viral replication
Protective or contribution to immunopathology 
n.d. Viral Z-RNA (e.g., subgenomic RNA) Karki et al., 2022; Kuriakose et al., 2016; Momota et al., 2020; Thapa et al., 2016; Zhang et al., 2020  
SARS-CoV-2 Coronaviridae (+ssRNA) Apoptosis and necroptosis (and pyroptosis in BMDMs)
Contribution to inflammatory cytokine production 
No effect on viral replication
Causes immunopathology 
n.d. Viral Z-RNA Li et al., 2023; Peng et al., 2022  
MHV Apoptosis and necroptosis (and pyroptosis in BMDMs) Causes immunopathology following IFN-β treatment n.d. n.d. Karki et al., 2022  
Zika virus Flaviviridae (+ssRNA) IRF1-dependent upregulation of IRG1 resulting in a metabolic antiviral state in neurons Restriction of viral replication, better survival n.d. n.d. Daniels et al., 2019; Rothan et al., 2019  
West Nile virus Flaviviridae (+ssRNA) n.d. Restriction of viral replication, better survival n.d. n.d. Rothan et al., 2019  
VirusFamilyProposed antiviral function(s)Role(s) in in vivo mouse modelsViral ZBP1 antagonistZBP1 agonistReferences
MCMV Herpesviridae (large dsDNA) Necroptosis Restriction of viral replication
Protective 
M45 (vIRA) inhibits necroptosis RNA Jiao et al., 2020; Maelfait et al., 2017; Sridharan et al., 2017; Upton et al., 2012  
UV-inactivated human cytomegalovirus Contribution to type I IFN response n.a. UL36 potentially inhibits necroptosis n.d. DeFilippis et al., 2010  
HSV-1 Necroptosis and apoptosis (and pyroptosis in BMDMs)
Contribution to type I IFN response and inflammatory cytokine production
Potential inhibition of ICP0-mediated degradation of IFI16 
Restriction of viral replication ICP6 (UL39) inhibits necroptosis n.d. Furr et al., 2011; Guo et al., 2018; Jeffries et al., 2022; Lee et al., 2021; Pham et al., 2013; Takaoka et al., 2007  
HSV-2 Necroptosis?
Contribution to type I IFN response 
n.d. ICP10 (UL39) potentially inhibits necroptosis n.d. Triantafilou et al., 2014  
VZV Apoptosis n.d. Triplex capsid subunit 1 (ORF20) inhibits caspase-dependent cell death n.d. Steain et al., 2020  
Vaccinia virus Poxviridae (large dsDNA) Necroptosis Restriction of viral replication
Protective 
E3 (E3L) sequesters Z-RNA Z-RNA Koehler et al., 2017, 2021  
IAV Orthomyxoviridae (−ssRNA) Apoptosis and necroptosis (and pyroptosis in BMDMs)
Contribution to inflammatory cytokine production 
Restriction of viral replication
Protective or contribution to immunopathology 
n.d. Viral Z-RNA (e.g., subgenomic RNA) Karki et al., 2022; Kuriakose et al., 2016; Momota et al., 2020; Thapa et al., 2016; Zhang et al., 2020  
SARS-CoV-2 Coronaviridae (+ssRNA) Apoptosis and necroptosis (and pyroptosis in BMDMs)
Contribution to inflammatory cytokine production 
No effect on viral replication
Causes immunopathology 
n.d. Viral Z-RNA Li et al., 2023; Peng et al., 2022  
MHV Apoptosis and necroptosis (and pyroptosis in BMDMs) Causes immunopathology following IFN-β treatment n.d. n.d. Karki et al., 2022  
Zika virus Flaviviridae (+ssRNA) IRF1-dependent upregulation of IRG1 resulting in a metabolic antiviral state in neurons Restriction of viral replication, better survival n.d. n.d. Daniels et al., 2019; Rothan et al., 2019  
West Nile virus Flaviviridae (+ssRNA) n.d. Restriction of viral replication, better survival n.d. n.d. Rothan et al., 2019  

Viruses known to be detected by ZBP1 are shown, along with viral families and proposed antiviral functions of ZBP1. Where these have been determined, in vivo roles of ZBP1, viral ZBP1 antagonists, and nucleic acid agonists of ZBP1 are given. n.d., not determined; n.a., not applicable.

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