Primer sequences (5′ to 3′) used for sgRNAs
| . | . |
|---|---|
| BUD13-sg2-F: | 5′-CACCGTCTGAAGCGTTACTTGTCCG-3′ |
| BUD13-sg2-R: | 5′-AAACCGGACAAGTAACGCTTCAGAC-3′ |
| BUD13-sg3-F: | 5′-CACCGCACGGAATAGACCTAGACAC-3′ |
| BUD13-sg3-R: | 5′-AAACGTGTCTAGGTCTATTCCGTGC-3′ |
| BUD13-sg4-F: | 5′-CACCGTCATGACGGGCCCTCCTTGG-3′ |
| BUD13-sg4-R: | 5′-AAACCCAAGGAGGGCCCGTCATGAC-3′ |
| PRKACB-sg1-F: | 5′-CACCGTACTCCAGTCGAACAAGGAA-3′ |
| PRKACB-sg1-R: | 5′-AAACTTCCTTGTTCGACTGGAGTAC-3′ |
| PRKACB-sg2-F: | 5′-CACCGTGGTTATGGAATATGTCCCT-3′ |
| PRKACB-sg2-R: | 5′-AAACAGGGACATATTCCATAACCAC-3′ |
| PRKACB-sg3-F: | 5′-CACCGACCCTTGGAACAGGTTCATT-3′ |
| PRKACB-sg3-R: | 5′-AAACAATGAACCTGTTCCAAGGGTC-3′ |
| PKC1-sg1-F: | 5′-CACCGGCTGTTTTGTGGTCCACAAG-3′ |
| PKC1-sg1-R: | 5′-AAACCTTGTGGACCACAAAACAGCC-3′ |
| PKC1-sg2-F: | 5′-CACCGTCTATGGACTTATCCATCAA-3′ |
| PKC1-sg2-R: | 5′-AAACTTGATGGATAAGTCCATAGAC-3′ |
| PKC1-sg3-F: | 5′-CACCGGAAGAACGTGCACGAGGTGA-3′ |
| PKC1-sg3-R: | 5′-AAACTCACCTCGTGCACGTTCTTCC-3′ |
| PKC2-sg1-F: | 5′-CACCGGCTGTATGGACTCATCCACC-3′ |
| PKC2-sg1-R: | 5′-AAACGGTGGATGAGTCCATACAGCC-3′ |
| PKC2-sg2-F: | 5′-CACCGGCTGCTTTGTGGTGCACAAG-3′ |
| PKC2-sg2-R: | 5'-AAACCTTGTGCACCACAAAGCAGCC-3' |
| PKC2-sg3-F: | 5′-CACCGCCACGTTTTGTGACCACTGT-3′ |
| PKC2-sg3-R: | 5′-AAACACAGTGGTCACAAAACGTGGC-3′ |
| GRK6-sg1-F: | 5′-CACCGGTGCTGACACTGATGAACGG-3′ |
| GRK6-sg1-R: | 5′-AAACCCGTTCATCAGTGTCAGCACC-3′ |
| GRK6-sg2-F: | 5′-CACCGAAGTGACCCCGGATGACAAG-3′ |
| GRK6-sg2-R: | 5′-AAACCTTGTCATCCGGGGTCACTTC-3′ |
| GRK6-sg3-F: | 5′-CACCGGGTGCTGACACTGATGAACG-3′ |
| GRK6-sg3-R: | 5′-AAACCGTTCATCAGTGTCAGCACCC-3′ |
| PKN3-sg1-F: | 5′-CACCGAAGCAGTTTCACCACGTTCT-3′ |
| PKN3-sg1-R: | 5′-AAACAGAACGTGGTGAAACTGCTTC-3′ |
| PKN3-sg2-F: | 5′-CACCGAACACAGGACCGCAAGGCAC-3′ |
| PKN3-sg2-R: | 5′-AAACGTGCCTTGCGGTCCTGTGTTC-3' |
| PKN3-sg3-F: | 5′-CACCGATGACCCACACGTGCGCCAG-3′ |
| PKN3-sg3-R: | 5′-AAACCTGGCGCACGTGTGGGTCATC-3′ |
| RSK3-sg1-F: | 5′-CACCGGTTTTAGGACAAGGATCCTA-3′ |
| RSK3-sg1-R: | 5′-AAACTAGGATCCTTGTCCTAAAACC-3′ |
| RSK3-sg2-F: | 5′-CACCGGGCTTTCTTAAGGACCTTCA-3′ |
| RSK3-sg2-R: | 5′-AAACTGAAGGTCCTTAAGAAAGCCC-3′ |
| RSK3-sg3-F: | 5′-CACCGCTGCTGAAGGTTTTAGGACA-3′ |
| RSK3-sg3-R: | 5′-AAACTGTCCTAAAACCTTCAGCAGC-3′ |
| RSK3-sg4-F: | 5′-CACCGTTGGACACGACGTCCTGCCA-3′ |
| RSK3-sg4-R: | 5′-AAACTGGCAGGACGTCGTGTCCAAC-3′ |
| RPS6KA4-sg1-F: | 5′-CACCGAAATCATCCGTAGCAAGACG-3′ |
| RPS6KA4-sg1-R: | 5′-AAACCGTCTTGCTACGGATGATTTC-3′ |
| RPS6KA4-sg2-F: | 5′-CACCGGAAATCATCCGTAGCAAGAC-3′ |
| RPS6KA4-sg2-R: | 5′-AAACGTCTTGCTACGGATGATTTCC-3′ |
| RPS6KA4-sg3-F: | 5′-CACCGAATGTGCTGCTGGACTCCGA-3′ |
| RPS6KA4-sg3-R: | 5′-AAACTCGGAGTCCAGCAGCACATTC-3′ |
| c-Jun-sg1-F: | 5′-CACCGTGCTCTGTTTCAGGATCTTG-3′ |
| c-Jun-sg1-R: | 5′-AAACCAAGATCCTGAAACAGAGCAC-3′ |
| c-Jun-sg3-F: | 5′-CACCGCAAGCTGGCGTCGCCCGAGC-3′ |
| c-Jun-sg3-R: | 5′-AAACGCTCGGGCGACGCCAGCTTGC-3′ |
| Rictor-sg-F: | 5′-CACCGAAGTGGACTATTTAATAGCT-3′ |
| Rictor-sg-R: | 5′-AAACTAGTAAAGTTATTCAGATGGC-3′ |
| . | . |
|---|---|
| BUD13-sg2-F: | 5′-CACCGTCTGAAGCGTTACTTGTCCG-3′ |
| BUD13-sg2-R: | 5′-AAACCGGACAAGTAACGCTTCAGAC-3′ |
| BUD13-sg3-F: | 5′-CACCGCACGGAATAGACCTAGACAC-3′ |
| BUD13-sg3-R: | 5′-AAACGTGTCTAGGTCTATTCCGTGC-3′ |
| BUD13-sg4-F: | 5′-CACCGTCATGACGGGCCCTCCTTGG-3′ |
| BUD13-sg4-R: | 5′-AAACCCAAGGAGGGCCCGTCATGAC-3′ |
| PRKACB-sg1-F: | 5′-CACCGTACTCCAGTCGAACAAGGAA-3′ |
| PRKACB-sg1-R: | 5′-AAACTTCCTTGTTCGACTGGAGTAC-3′ |
| PRKACB-sg2-F: | 5′-CACCGTGGTTATGGAATATGTCCCT-3′ |
| PRKACB-sg2-R: | 5′-AAACAGGGACATATTCCATAACCAC-3′ |
| PRKACB-sg3-F: | 5′-CACCGACCCTTGGAACAGGTTCATT-3′ |
| PRKACB-sg3-R: | 5′-AAACAATGAACCTGTTCCAAGGGTC-3′ |
| PKC1-sg1-F: | 5′-CACCGGCTGTTTTGTGGTCCACAAG-3′ |
| PKC1-sg1-R: | 5′-AAACCTTGTGGACCACAAAACAGCC-3′ |
| PKC1-sg2-F: | 5′-CACCGTCTATGGACTTATCCATCAA-3′ |
| PKC1-sg2-R: | 5′-AAACTTGATGGATAAGTCCATAGAC-3′ |
| PKC1-sg3-F: | 5′-CACCGGAAGAACGTGCACGAGGTGA-3′ |
| PKC1-sg3-R: | 5′-AAACTCACCTCGTGCACGTTCTTCC-3′ |
| PKC2-sg1-F: | 5′-CACCGGCTGTATGGACTCATCCACC-3′ |
| PKC2-sg1-R: | 5′-AAACGGTGGATGAGTCCATACAGCC-3′ |
| PKC2-sg2-F: | 5′-CACCGGCTGCTTTGTGGTGCACAAG-3′ |
| PKC2-sg2-R: | 5'-AAACCTTGTGCACCACAAAGCAGCC-3' |
| PKC2-sg3-F: | 5′-CACCGCCACGTTTTGTGACCACTGT-3′ |
| PKC2-sg3-R: | 5′-AAACACAGTGGTCACAAAACGTGGC-3′ |
| GRK6-sg1-F: | 5′-CACCGGTGCTGACACTGATGAACGG-3′ |
| GRK6-sg1-R: | 5′-AAACCCGTTCATCAGTGTCAGCACC-3′ |
| GRK6-sg2-F: | 5′-CACCGAAGTGACCCCGGATGACAAG-3′ |
| GRK6-sg2-R: | 5′-AAACCTTGTCATCCGGGGTCACTTC-3′ |
| GRK6-sg3-F: | 5′-CACCGGGTGCTGACACTGATGAACG-3′ |
| GRK6-sg3-R: | 5′-AAACCGTTCATCAGTGTCAGCACCC-3′ |
| PKN3-sg1-F: | 5′-CACCGAAGCAGTTTCACCACGTTCT-3′ |
| PKN3-sg1-R: | 5′-AAACAGAACGTGGTGAAACTGCTTC-3′ |
| PKN3-sg2-F: | 5′-CACCGAACACAGGACCGCAAGGCAC-3′ |
| PKN3-sg2-R: | 5′-AAACGTGCCTTGCGGTCCTGTGTTC-3' |
| PKN3-sg3-F: | 5′-CACCGATGACCCACACGTGCGCCAG-3′ |
| PKN3-sg3-R: | 5′-AAACCTGGCGCACGTGTGGGTCATC-3′ |
| RSK3-sg1-F: | 5′-CACCGGTTTTAGGACAAGGATCCTA-3′ |
| RSK3-sg1-R: | 5′-AAACTAGGATCCTTGTCCTAAAACC-3′ |
| RSK3-sg2-F: | 5′-CACCGGGCTTTCTTAAGGACCTTCA-3′ |
| RSK3-sg2-R: | 5′-AAACTGAAGGTCCTTAAGAAAGCCC-3′ |
| RSK3-sg3-F: | 5′-CACCGCTGCTGAAGGTTTTAGGACA-3′ |
| RSK3-sg3-R: | 5′-AAACTGTCCTAAAACCTTCAGCAGC-3′ |
| RSK3-sg4-F: | 5′-CACCGTTGGACACGACGTCCTGCCA-3′ |
| RSK3-sg4-R: | 5′-AAACTGGCAGGACGTCGTGTCCAAC-3′ |
| RPS6KA4-sg1-F: | 5′-CACCGAAATCATCCGTAGCAAGACG-3′ |
| RPS6KA4-sg1-R: | 5′-AAACCGTCTTGCTACGGATGATTTC-3′ |
| RPS6KA4-sg2-F: | 5′-CACCGGAAATCATCCGTAGCAAGAC-3′ |
| RPS6KA4-sg2-R: | 5′-AAACGTCTTGCTACGGATGATTTCC-3′ |
| RPS6KA4-sg3-F: | 5′-CACCGAATGTGCTGCTGGACTCCGA-3′ |
| RPS6KA4-sg3-R: | 5′-AAACTCGGAGTCCAGCAGCACATTC-3′ |
| c-Jun-sg1-F: | 5′-CACCGTGCTCTGTTTCAGGATCTTG-3′ |
| c-Jun-sg1-R: | 5′-AAACCAAGATCCTGAAACAGAGCAC-3′ |
| c-Jun-sg3-F: | 5′-CACCGCAAGCTGGCGTCGCCCGAGC-3′ |
| c-Jun-sg3-R: | 5′-AAACGCTCGGGCGACGCCAGCTTGC-3′ |
| Rictor-sg-F: | 5′-CACCGAAGTGGACTATTTAATAGCT-3′ |
| Rictor-sg-R: | 5′-AAACTAGTAAAGTTATTCAGATGGC-3′ |