Primer sequences (5′ to 3′) used for vector construction
| . | . |
|---|---|
| BUD13-XhoI-F | 5′-GCATCTCGAGCGGCGGCAGCTCCGCCG-3′ |
| BUD13-EcoRI-F | 5′-GCATGAATTCCGGCGGCAGCTCCGCCG-3′ |
| BUD13-NotI-R | 5′-GCATGCGGCCGCTTACATATCCTCAACACTCCATTTGTAGGC-3′ |
| BUD13-XbaI-F | 5′-GCATGCGGCCGCTTACATATCCTCAACACTCCATTTGTAGGC-3′ |
| BUD13-HA-XbaI-F | 5′-GCATTCTAGAGCCACCATGTACCCATACGATGTTCCAGATTACGCTGCGGCAGCTCCGC-3′ |
| BUD13-R230Q-F | 5′-CCCTCTCCTCCTAGGCAAGCCCGTCATGGTTCC-3′ |
| BUD13-R230Q-R | 5-GGAACCATGACGGGCTTGCCTAGGAGGAGAGGG-3′ |
| BUD13-R156P-F | 5′-CCTAGGAGGGCCCCTCATGACACCCCGGATCC-3′ |
| BUD13-R156P-R | 5′-GGATCCGGGGTGTCATGAGGGGCCCTCCTAGG-3′ |
| BUD13-R156C-F | 5′-CCTAGGAGGGCCTGTCATGACACCCCGGATCC-3′ |
| BUD13-R156C-R | 5′-GGATCCGGGGTGTCATGACAGGCCCTCCTAGG-3′ |
| BUD13-R156CT159E-F | 5′-CCTAGGAGGGCCTGTCATGACGAGCCGGATCC-3′ |
| BUD13-R156CT159E-R | 5′-GGATCCGGCTCGTCATGACAGGCCCTCCTAGG-3′ |
| Biotin-BUD13-RNA-F | biotin-5′-AUCAGUACCAGGUGAGTCUAUCAUG-3′ |
| Biotin-BUD13-RNA-R | 5′-CATGATAGACTCACCTG GTACTGAT-3′ |
| hcJun-BamHI-F | 5′-GCATGGATCCACTGCAAAGATGGAAACGACCTTC-3′ |
| hcJun-SalI-R | 5′-GCATGTCGACTCAAAATGTTTGCAACTGCTGCG-3′ |
| hcMyc-BamHI-F | 5′-GCATGGATCCCCCCTCAACGTTAGCTTCAC-3′ |
| hcMyc-SalI-R | 5′-GCATGTCGACTTACGCACAAGAGTTCCGTAGC-3′ |
| Fbw7-BglII-F | 5′-GCATAGATCTAATCAGGAACTGCTCTCTGTGG-3′ |
| Fbw7-SalI-R | 5′-GCATGTCGACTCACTTCATGTCCACATCAAAGTCC-3′ |
| . | . |
|---|---|
| BUD13-XhoI-F | 5′-GCATCTCGAGCGGCGGCAGCTCCGCCG-3′ |
| BUD13-EcoRI-F | 5′-GCATGAATTCCGGCGGCAGCTCCGCCG-3′ |
| BUD13-NotI-R | 5′-GCATGCGGCCGCTTACATATCCTCAACACTCCATTTGTAGGC-3′ |
| BUD13-XbaI-F | 5′-GCATGCGGCCGCTTACATATCCTCAACACTCCATTTGTAGGC-3′ |
| BUD13-HA-XbaI-F | 5′-GCATTCTAGAGCCACCATGTACCCATACGATGTTCCAGATTACGCTGCGGCAGCTCCGC-3′ |
| BUD13-R230Q-F | 5′-CCCTCTCCTCCTAGGCAAGCCCGTCATGGTTCC-3′ |
| BUD13-R230Q-R | 5-GGAACCATGACGGGCTTGCCTAGGAGGAGAGGG-3′ |
| BUD13-R156P-F | 5′-CCTAGGAGGGCCCCTCATGACACCCCGGATCC-3′ |
| BUD13-R156P-R | 5′-GGATCCGGGGTGTCATGAGGGGCCCTCCTAGG-3′ |
| BUD13-R156C-F | 5′-CCTAGGAGGGCCTGTCATGACACCCCGGATCC-3′ |
| BUD13-R156C-R | 5′-GGATCCGGGGTGTCATGACAGGCCCTCCTAGG-3′ |
| BUD13-R156CT159E-F | 5′-CCTAGGAGGGCCTGTCATGACGAGCCGGATCC-3′ |
| BUD13-R156CT159E-R | 5′-GGATCCGGCTCGTCATGACAGGCCCTCCTAGG-3′ |
| Biotin-BUD13-RNA-F | biotin-5′-AUCAGUACCAGGUGAGTCUAUCAUG-3′ |
| Biotin-BUD13-RNA-R | 5′-CATGATAGACTCACCTG GTACTGAT-3′ |
| hcJun-BamHI-F | 5′-GCATGGATCCACTGCAAAGATGGAAACGACCTTC-3′ |
| hcJun-SalI-R | 5′-GCATGTCGACTCAAAATGTTTGCAACTGCTGCG-3′ |
| hcMyc-BamHI-F | 5′-GCATGGATCCCCCCTCAACGTTAGCTTCAC-3′ |
| hcMyc-SalI-R | 5′-GCATGTCGACTTACGCACAAGAGTTCCGTAGC-3′ |
| Fbw7-BglII-F | 5′-GCATAGATCTAATCAGGAACTGCTCTCTGTGG-3′ |
| Fbw7-SalI-R | 5′-GCATGTCGACTCACTTCATGTCCACATCAAAGTCC-3′ |