List of moss strains used in this study
| Name . | Experimental use . | Main clone used . | Background strain . | Plasmid transformed . | Figure . | Reference . |
|---|---|---|---|---|---|---|
| Kin4-Ia-citrine | Generating Kinesin4-Ia + antiparallel overlap colocalization strain | #43 | WT | pTM374 | - | Miki et al., 2014; de Keijzer et al., 2017 |
| Kin4-Ia-citrine/MAP65a-mCherry | Kinesin4-Ia + antiparallel overlap colocalization | #121 | Kin4-Ia-citrine | MAP65a-mCherry | 1, B and C; 3, E–I; S2, A and E | This study |
| MAP65a-citrine | Overlap visualization/Kin4-Ia knock-out construction | #2 | WT | See reference | 1, D–G, 2; S2, B and F–I; S5, A and B | Kosetsu et al., 2013 |
| MAP65a-citrine/∆kin4-Ia | Measuring effect of Kin4-Ia removal on overlap dimension and cell tip growth | #7 and #122 | MAP65a-citrine | See reference | 1, D–G; 2; S2, B and F–I; S5, A and B | de Keijzer et al., 2017 |
| MAP65a-citrine/∆kin4-Ic | Measuring effect of Kin4-Ic removal on overlap dimension | #172 | MAP65a-citrine | See reference | S2 D | de Keijzer et al., 2017 |
| MAP65a-citrine/∆kin4-Ic/∆kin4-Ia | Measuring effect of combined Kin4-Ia and Kin4-Ic removal on overlap dimension | #66 | MAP65a-citrine/∆kin4-Ic | See reference | S2 D | de Keijzer et al., 2017 |
| MAP65c-citrine | Generating overlap + EB1b co-localization strain | #5 | WT | See reference | - | Kosetsu et al., 2013 |
| MAP65c-citrine/MAP65a-mCherry | Co-localization of different MAP65 paralogs at overlaps | #90 | MAP65c-citrine | MAP65a-mCherry | S2, A and C | This study |
| MAP65c-citrine/EB1b-mCherry | Polymerization visualization in overlaps/plus end tracking | #100 | MAP65c-citrine | EB1b-mCherry | 3, A–C; 4, A–C; S3, A–D; S4 | This study |
| MAP65a-citrine/∆kin4-Ia/EB1b-mCherry | Measuring effect of Kin4-Ia removal on polymerization dynamics in overlaps and orientation of polymerization in general | #96 | MAP65a-citrine/∆kin4-Ia #7 | EB1b-mCherry | 3, A–C; 4, A–C; S3, A–D; S4 | This study |
| MAP65a-citrine/mCherry-α-tubulin | Microtubule cytoskeleton + antiparallel overlap visualization | #426 | MAP65a-citrine | EF1-mCherry-tua-BsdR | 1 A; 4, D and E; S1 A; S5, C–E | This study |
| MAP65a-citrine/∆kin4-Ia/mCherry-α-tubulin | Measuring effect of Kin4-Ia removal on microtubule-network architecture and apical focussing | #14 | MAP65a-citrine/∆kin4-Ia #7 | EF1-mCherry-tua-BsdR | 4, D and E; S5, C–E | This study |
| EF1-mCherry-α-tubulin | Generating Kin4-Ia/Kin4-Ic + microtubule array colocalization strains | #52 | WT | EF1-mCherry-tua-BsdR | - | Kosetsu et al., 2017 |
| EF1-mCherry-α-tubulin/Kin4-Ia-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #22 | EF1-mCherry-α-tubulin | pTM374 | S1B | This study; Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-Ib-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #12 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| EF1-mCherry-α-tubulin/Kin4-Ic-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #19 | EF1-mCherry-α-tubulin | pTM365 | S1B | This study; Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-Id-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #8 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-Ie-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #14 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-IIa-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #2 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-IIb-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #5 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-IIc-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #9 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| Name . | Experimental use . | Main clone used . | Background strain . | Plasmid transformed . | Figure . | Reference . |
|---|---|---|---|---|---|---|
| Kin4-Ia-citrine | Generating Kinesin4-Ia + antiparallel overlap colocalization strain | #43 | WT | pTM374 | - | Miki et al., 2014; de Keijzer et al., 2017 |
| Kin4-Ia-citrine/MAP65a-mCherry | Kinesin4-Ia + antiparallel overlap colocalization | #121 | Kin4-Ia-citrine | MAP65a-mCherry | 1, B and C; 3, E–I; S2, A and E | This study |
| MAP65a-citrine | Overlap visualization/Kin4-Ia knock-out construction | #2 | WT | See reference | 1, D–G, 2; S2, B and F–I; S5, A and B | Kosetsu et al., 2013 |
| MAP65a-citrine/∆kin4-Ia | Measuring effect of Kin4-Ia removal on overlap dimension and cell tip growth | #7 and #122 | MAP65a-citrine | See reference | 1, D–G; 2; S2, B and F–I; S5, A and B | de Keijzer et al., 2017 |
| MAP65a-citrine/∆kin4-Ic | Measuring effect of Kin4-Ic removal on overlap dimension | #172 | MAP65a-citrine | See reference | S2 D | de Keijzer et al., 2017 |
| MAP65a-citrine/∆kin4-Ic/∆kin4-Ia | Measuring effect of combined Kin4-Ia and Kin4-Ic removal on overlap dimension | #66 | MAP65a-citrine/∆kin4-Ic | See reference | S2 D | de Keijzer et al., 2017 |
| MAP65c-citrine | Generating overlap + EB1b co-localization strain | #5 | WT | See reference | - | Kosetsu et al., 2013 |
| MAP65c-citrine/MAP65a-mCherry | Co-localization of different MAP65 paralogs at overlaps | #90 | MAP65c-citrine | MAP65a-mCherry | S2, A and C | This study |
| MAP65c-citrine/EB1b-mCherry | Polymerization visualization in overlaps/plus end tracking | #100 | MAP65c-citrine | EB1b-mCherry | 3, A–C; 4, A–C; S3, A–D; S4 | This study |
| MAP65a-citrine/∆kin4-Ia/EB1b-mCherry | Measuring effect of Kin4-Ia removal on polymerization dynamics in overlaps and orientation of polymerization in general | #96 | MAP65a-citrine/∆kin4-Ia #7 | EB1b-mCherry | 3, A–C; 4, A–C; S3, A–D; S4 | This study |
| MAP65a-citrine/mCherry-α-tubulin | Microtubule cytoskeleton + antiparallel overlap visualization | #426 | MAP65a-citrine | EF1-mCherry-tua-BsdR | 1 A; 4, D and E; S1 A; S5, C–E | This study |
| MAP65a-citrine/∆kin4-Ia/mCherry-α-tubulin | Measuring effect of Kin4-Ia removal on microtubule-network architecture and apical focussing | #14 | MAP65a-citrine/∆kin4-Ia #7 | EF1-mCherry-tua-BsdR | 4, D and E; S5, C–E | This study |
| EF1-mCherry-α-tubulin | Generating Kin4-Ia/Kin4-Ic + microtubule array colocalization strains | #52 | WT | EF1-mCherry-tua-BsdR | - | Kosetsu et al., 2017 |
| EF1-mCherry-α-tubulin/Kin4-Ia-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #22 | EF1-mCherry-α-tubulin | pTM374 | S1B | This study; Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-Ib-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #12 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| EF1-mCherry-α-tubulin/Kin4-Ic-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #19 | EF1-mCherry-α-tubulin | pTM365 | S1B | This study; Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-Id-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #8 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-Ie-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #14 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-IIa-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #2 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-IIb-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #5 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |
| Act-mCherry-α-tubulin/Kin4-IIc-citrine | Localization screen of all P. patens kinesin-4s in caulonemal tip cells | #9 | Act-mCherry-α-tubulin | See reference | S1B | Miki et al., 2014 |