Table 2.

List of primers used in this study

PrimerUseSequence (5′ > 3′)Restriction site
JK206 Kin4-Ia deletion genotyping 5′-TTT​GAG​GTG​TAT​CAC​CCA​GTT​TCG-3′ - 
JK207 Kin4-Ia citrine tagging genotyping 5′-GGA​GGA​AAG​TGA​TGG​CGA​AAG​AGC-3′ - 
JK208 Kin4-Ia deletion/citrine tagging genotyping 5′-GCA​GGA​AGC​ACG​AGC​TAA​GAC​C-3′ - 
JK161 Genotyping of MAP65a locus 5′-GGA​TGA​GAA​CCG​ATT​TGC​GAG​C-3′ - 
JK162 Genotyping of MAP65a locus 5′-GCG​GTA​AGA​AAG​TGG​AGA​GAA​GC-3′ - 
JK87 Cloning of EB1b left targeting arm 5′-GGG​GTA​CCC​AGC​TGA​TGG​ACG​TAG​TGC​ACC-3′ KpnI 
JK88 Cloning of EB1b left targeting arm 5′-CCC​AAG​CTT​CTC​TAA​AAG​ATC​CTT​ACT​GTC​GTC​G-3′ HindIII 
JK124 Cloning of EB1b right targeting arm 5′-TGT​TGA​CGG​CAC​TAT​TAC​TCG​C-3′ - 
JK90 Cloning of EB1b right targeting arm 5′-TCC​CCG​CGG​TAT​GGA​CAC​TCA​CCA​GGC​AAC​C-3′ SacII 
JK114 Sequence downstream of resistance cassette 5′-ACT​CAA​GAG​GAT​AAA​ACC​TCA​CC-3′ - 
JK111 Construct sequencing general 5′-GAT​TAA​GTT​GGG​TAA​CGC​CAG​G-3′ - 
JK116 Construct sequencing general 5′-ACA​CAG​GAA​ACA​GCT​ATG​ACC-3′ - 
JK129 Sequencing region upstream of mCherry 5′-CCC​TTG​GTC​ACC​TTC​AGC​TTG​G-3′ - 
JK156 Genotyping of EB1b locus 5′-CAT​TTG​TGG​GTA​TGT​GGG​TTG​G-3′ - 
JK157 Genotyping of EB1b locus 5′-TGA​CAA​AGG​GAG​AAT​AAA​AGA​GGT​GG-3′ - 
JK210 Kin4-Ic citrine tagging genotyping 5′-ACT​GAC​CTG​CCA​CAT​TCC​GAG​C-3′ - 
JK211 Kin4-Ic citrine tagging genotyping 5′-TTG​ACT​TGT​GTG​GAA​TCT​TGG​ATG​C-3′ - 
JK406 Cloning of Kin4-Ia-FL CDS (coding sequence) for Y2H 5′-CAC​CTG​CGA​AAC​AAT​GGA​GAG​AAC​CAA​C-3′ - 
JK407 Cloning of Kin4-Ia-414 CDS for Y2H 5′-CAC​CCA​GCG​CAT​GAG​GCA​TCA​GCT​G-3′ - 
JK408 Cloning of Kin4-Ia-727 CDS for Y2H 5′-CAC​CGT​CTG​GAA​AGC​ACA​AAG​AGA​GAA​G-3′ - 
JK409 Universal R primer for Kin4-Ia CDS cloning for Y2H 5′-TCA​CCG​CCG​TAA​AGT​GGA​ACG​AAT​G-3′ - 
HT124 Cloning of MAP65a CDS for Y2H 5′-CAC​CAT​GGG​CCA​GCA​ACC​TGG​A-3′ - 
HT125 Cloning of MAP65a CDS for Y2H 5′-TTA​CGC​GTC​GAT​ACA​GGG​AAC-3′ - 
HT126 Cloning of MAP65c CDS for Y2H 5′-CAC​CAT​GGG​TCA​GCA​ACC​TGT​TGT​C-3′ - 
HT127 Cloning of MAP65c CDS for Y2H 5′-TCA​GCT​GTT​CAT​GCT​TTT​CCA-3′ - 
PrimerUseSequence (5′ > 3′)Restriction site
JK206 Kin4-Ia deletion genotyping 5′-TTT​GAG​GTG​TAT​CAC​CCA​GTT​TCG-3′ - 
JK207 Kin4-Ia citrine tagging genotyping 5′-GGA​GGA​AAG​TGA​TGG​CGA​AAG​AGC-3′ - 
JK208 Kin4-Ia deletion/citrine tagging genotyping 5′-GCA​GGA​AGC​ACG​AGC​TAA​GAC​C-3′ - 
JK161 Genotyping of MAP65a locus 5′-GGA​TGA​GAA​CCG​ATT​TGC​GAG​C-3′ - 
JK162 Genotyping of MAP65a locus 5′-GCG​GTA​AGA​AAG​TGG​AGA​GAA​GC-3′ - 
JK87 Cloning of EB1b left targeting arm 5′-GGG​GTA​CCC​AGC​TGA​TGG​ACG​TAG​TGC​ACC-3′ KpnI 
JK88 Cloning of EB1b left targeting arm 5′-CCC​AAG​CTT​CTC​TAA​AAG​ATC​CTT​ACT​GTC​GTC​G-3′ HindIII 
JK124 Cloning of EB1b right targeting arm 5′-TGT​TGA​CGG​CAC​TAT​TAC​TCG​C-3′ - 
JK90 Cloning of EB1b right targeting arm 5′-TCC​CCG​CGG​TAT​GGA​CAC​TCA​CCA​GGC​AAC​C-3′ SacII 
JK114 Sequence downstream of resistance cassette 5′-ACT​CAA​GAG​GAT​AAA​ACC​TCA​CC-3′ - 
JK111 Construct sequencing general 5′-GAT​TAA​GTT​GGG​TAA​CGC​CAG​G-3′ - 
JK116 Construct sequencing general 5′-ACA​CAG​GAA​ACA​GCT​ATG​ACC-3′ - 
JK129 Sequencing region upstream of mCherry 5′-CCC​TTG​GTC​ACC​TTC​AGC​TTG​G-3′ - 
JK156 Genotyping of EB1b locus 5′-CAT​TTG​TGG​GTA​TGT​GGG​TTG​G-3′ - 
JK157 Genotyping of EB1b locus 5′-TGA​CAA​AGG​GAG​AAT​AAA​AGA​GGT​GG-3′ - 
JK210 Kin4-Ic citrine tagging genotyping 5′-ACT​GAC​CTG​CCA​CAT​TCC​GAG​C-3′ - 
JK211 Kin4-Ic citrine tagging genotyping 5′-TTG​ACT​TGT​GTG​GAA​TCT​TGG​ATG​C-3′ - 
JK406 Cloning of Kin4-Ia-FL CDS (coding sequence) for Y2H 5′-CAC​CTG​CGA​AAC​AAT​GGA​GAG​AAC​CAA​C-3′ - 
JK407 Cloning of Kin4-Ia-414 CDS for Y2H 5′-CAC​CCA​GCG​CAT​GAG​GCA​TCA​GCT​G-3′ - 
JK408 Cloning of Kin4-Ia-727 CDS for Y2H 5′-CAC​CGT​CTG​GAA​AGC​ACA​AAG​AGA​GAA​G-3′ - 
JK409 Universal R primer for Kin4-Ia CDS cloning for Y2H 5′-TCA​CCG​CCG​TAA​AGT​GGA​ACG​AAT​G-3′ - 
HT124 Cloning of MAP65a CDS for Y2H 5′-CAC​CAT​GGG​CCA​GCA​ACC​TGG​A-3′ - 
HT125 Cloning of MAP65a CDS for Y2H 5′-TTA​CGC​GTC​GAT​ACA​GGG​AAC-3′ - 
HT126 Cloning of MAP65c CDS for Y2H 5′-CAC​CAT​GGG​TCA​GCA​ACC​TGT​TGT​C-3′ - 
HT127 Cloning of MAP65c CDS for Y2H 5′-TCA​GCT​GTT​CAT​GCT​TTT​CCA-3′ - 
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