List of plasmids used in this study
| Plasmid . | Usage . | Source . |
|---|---|---|
| MAP65a-mCherry | C-terminal mCherry tagging of MAP65a | de Keijzer et al., 2017 |
| EF1-mCherry-tua-BsdR (pTM426) | EF1α-promoter driven expression of mCherry-α-tubulin | Kosetsu et al., 2017 |
| EB1b-mCherry | C-terminal mCherry tagging of expressed EB1-homolog EB1b | This study |
| pTM374 | C-terminal citrine tagging of Kin4-Ia | Miki et al., 2014 |
| pTM365 | C-terminal citrine tagging of Kin4-Ic | Miki et al., 2014 |
| pDEST22/32-MAP65a | Activation domain and binding domain (AD/BD) fusion to MAP65a for use in Y2H | This study |
| pDEST22/32-MAP65c | AD/BD fusion to MAP65c for use in Y2H | This study |
| pDEST22/32-Kin4-Ia-FL | AD/BD fusion to FL Kin4-Ia for use in Y2H | This study |
| pDEST22/32-Kin4-Ia-414 | AD/BD fusion to Kin4-Ia C-terminal fragment after 414th amino acid for use in Y2H | This study |
| pDEST22/32-Kin4-Ia-727 | AD/BD fusion to Kin4-Ia C-terminal fragment after 727th amino acid for use in Y2H | This study |
| Plasmid . | Usage . | Source . |
|---|---|---|
| MAP65a-mCherry | C-terminal mCherry tagging of MAP65a | de Keijzer et al., 2017 |
| EF1-mCherry-tua-BsdR (pTM426) | EF1α-promoter driven expression of mCherry-α-tubulin | Kosetsu et al., 2017 |
| EB1b-mCherry | C-terminal mCherry tagging of expressed EB1-homolog EB1b | This study |
| pTM374 | C-terminal citrine tagging of Kin4-Ia | Miki et al., 2014 |
| pTM365 | C-terminal citrine tagging of Kin4-Ic | Miki et al., 2014 |
| pDEST22/32-MAP65a | Activation domain and binding domain (AD/BD) fusion to MAP65a for use in Y2H | This study |
| pDEST22/32-MAP65c | AD/BD fusion to MAP65c for use in Y2H | This study |
| pDEST22/32-Kin4-Ia-FL | AD/BD fusion to FL Kin4-Ia for use in Y2H | This study |
| pDEST22/32-Kin4-Ia-414 | AD/BD fusion to Kin4-Ia C-terminal fragment after 414th amino acid for use in Y2H | This study |
| pDEST22/32-Kin4-Ia-727 | AD/BD fusion to Kin4-Ia C-terminal fragment after 727th amino acid for use in Y2H | This study |