Table 2.

Guide-RNAs for CRISPR/Cas9 gene knockout assays

sgRNAOriginal sequence 5′–3′BsmBI site added
Ifngr1 sgRNA 1 5′-CCA​GGA​AAG​GTC​GGG​CCC​CC-3′ 5′-CAC​CGC​CAG​GAA​AGG​TCG​GGC​CCC​C-3′ 
Complementary 5′-GGG​GGC​CCG​ACC​TTT​CCT​GG-3′ 5′-AAA​CGG​GGG​CCC​GAC​CTT​TCC​TGG​C-3′ 
Ifngr1 sgRNA 2 5′-TGG​TAT​TCC​CAG​CAT​ACG​AC-3′ 5′-CAC​CGT​GGT​ATT​CCC​AGC​ATA​CGA​C-3′ 
Complementary 5′-GTC​GTA​TGC​TGG​GAA​TAC​CA-3′ 5′-AAA​CGT​CGT​ATG​CTG​GGA​ATA​CCA​C-3′ 
Ifngr1 sgRNA 3 5′-GAT​CCT​ACA​TAC​GAA​ACA​TA-3′ 5′-CAC​CGG​ATC​CTA​CAT​ACG​AAA​CAT​A-3′ 
Complementary 5′-TAT​GTT​TCG​TAT​GTA​GGA​TC-3′ 5′-AAA​CTA​TGT​TTC​GTA​TGT​AGG​ATC​C-3′ 
Ifngr2 sgRNA 1 5′-TCT​GTG​ATG​TCC​GTA​CAG​TT-3′ 5′-CAC​CGT​CTG​TGA​TGT​CCG​TAC​AGT​T-3′ 
Complementary 5′-AAC​TGT​ACG​GAC​ATC​ACA​GA-3′ 5′-AAA​CAA​CTG​TAC​GGA​CAT​CAC​AGA​C-3′ 
Ifngr2 sgRNA 2 5′-TTC​AGT​ATC​TTG​TCC​ACT​AC-3′ 5′-CAC​CGT​TCA​GTA​TCT​TGT​CCA​CTA​C-3′ 
Complementary 5′-GTA​GTG​GAC​AAG​ATA​CTG​AA-3′ 5′-AAA​CGT​AGT​GGA​CAA​GAT​ACT​GAA​C-3′ 
Ifngr2 sgRNA 3 5′-CAC​TGG​TCT​TGG​GTC​ATT​GC-3′ 5′-CAC​CGC​ACT​GGT​CTT​GGG​TCA​TTG​C-3′ 
Complementary 5′-GCA​ATG​ACC​CAA​GAC​CAG​TG-3′ 5′-AAA​CGC​AAT​GAC​CCA​AGA​CCA​GTG​C-3′ 
Ciita sgRNA 1 5′-ATA​GAG​ATC​CCT​GTA​GAA​GC-3′ 5′-CAC​CGA​TAG​AGA​TCC​CTG​TAG​AAG​C-3′ 
Complementary 5′-GCT​TCT​ACA​GGG​ATC​TCT​AT-3′ 5′-AAA​CGC​TTC​TAC​AGG​GAT​CTC​TAT​C-3′ 
Ciita sgRNA 2 5′-CCA​GTT​CAG​CAA​GCT​GTT​GC-3′ 5′-CAC​CGC​CAG​TTC​AGC​AAG​CTG​TTG​C-3′ 
Complementary 5′-GCA​ACA​GCT​TGC​TGA​ACT​GG-3′ 5′-AAA​CGC​AAC​AGC​TTG​CTG​AAC​TGG​C-3′ 
Ciita sgRNA 3 5′-TAC​TGA​AGA​GGT​CCT​TGC​TC-3′ 5′-CAC​CGT​ACT​GAA​GAG​GTC​CTT​GCT​C-3′ 
Complementary 5′-GAG​CAA​GGA​CCT​CTT​CAG​TA-3′ 5′-AAA​CGA​GCA​AGG​ACC​TCT​TCA​GTA​C-3′ 
H2-Aa sgRNA1 5′-ATA​GCA​AGT​CAG​TCG​CAG​A-3′ 5′-CAC​CGA​TAG​CAA​GTC​AGT​CGC​AGA-3′ 
Complementary 5′-TCT​GCG​ACT​GAC​TTG​CTA​T-3′ 5′-AAA​CTC​TGC​GAC​TGA​CTT​GCT​ATC-3′ 
H2-Aa sgRNA2 5′-TAT​ACC​ATA​GGT​GCC​TAC​G-3′ 5′-CAC​CGT​ATA​CCA​TAG​GTG​CCT​ACG-3′ 
Complementary 5′-CGT​AGG​CAC​CTA​TGG​TAT​A-3′ 5′-AAA​CCG​TAG​GCA​CCT​ATG​GTA​TAC-3′ 
H2-Ab1 sgRNA1 5′-CGT​ATG​CGC​TGC​GTC​CCG​T-3′ 5′-CAC​CGC​GTA​TGC​GCT​GCG​TCC​CGT-3′ 
Complementary 5′-ACG​GGA​CGC​AGC​GCA​TAC​G-3′ 5′-AAA​CAC​GGG​ACG​CAG​CGC​ATA​CGC-3′ 
H2-Ab1 sgRNA2 5′-GCG​GCC​AGA​CGC​CGA​GTA​C-3′ 5′-CAC​CGG​CGG​CCA​GAC​GCC​GAG​TAC-3′ 
Complementary 5′-GTA​CTC​GGC​GTC​TGG​CCG​C-3′ 5′-AAA​CGT​ACT​CGG​CGT​CTG​GCC​GCC-3′ 
H2-Eb1 sgRNA1 5′-CAA​CGG​GAC​GCA​GCG​CGT​G-3′ 5′-CAC​CGC​AAC​GGG​ACG​CAG​CGC​GTG-3′ 
Complementary 5′-CAC​GCG​CTG​CGT​CCC​GTT​G-3′ 5′-AAA​CCA​CGC​GCT​GCG​TCC​CGT​TGC-3′ 
H2-Eb1 sgRNA2 5′-GAC​GTG​GGC​GAG​TTC​CGC​G-3′ 5′-CAC​CGG​ACG​TGG​GCG​AGT​TCC​GCG-3′ 
Complementary 5′-CGC​GGA​ACT​CGC​CCA​CGT​C-3′ 5′-AAA​CCG​CGG​AAC​TCG​CCC​ACG​TCC-3′ 
sgRNAOriginal sequence 5′–3′BsmBI site added
Ifngr1 sgRNA 1 5′-CCA​GGA​AAG​GTC​GGG​CCC​CC-3′ 5′-CAC​CGC​CAG​GAA​AGG​TCG​GGC​CCC​C-3′ 
Complementary 5′-GGG​GGC​CCG​ACC​TTT​CCT​GG-3′ 5′-AAA​CGG​GGG​CCC​GAC​CTT​TCC​TGG​C-3′ 
Ifngr1 sgRNA 2 5′-TGG​TAT​TCC​CAG​CAT​ACG​AC-3′ 5′-CAC​CGT​GGT​ATT​CCC​AGC​ATA​CGA​C-3′ 
Complementary 5′-GTC​GTA​TGC​TGG​GAA​TAC​CA-3′ 5′-AAA​CGT​CGT​ATG​CTG​GGA​ATA​CCA​C-3′ 
Ifngr1 sgRNA 3 5′-GAT​CCT​ACA​TAC​GAA​ACA​TA-3′ 5′-CAC​CGG​ATC​CTA​CAT​ACG​AAA​CAT​A-3′ 
Complementary 5′-TAT​GTT​TCG​TAT​GTA​GGA​TC-3′ 5′-AAA​CTA​TGT​TTC​GTA​TGT​AGG​ATC​C-3′ 
Ifngr2 sgRNA 1 5′-TCT​GTG​ATG​TCC​GTA​CAG​TT-3′ 5′-CAC​CGT​CTG​TGA​TGT​CCG​TAC​AGT​T-3′ 
Complementary 5′-AAC​TGT​ACG​GAC​ATC​ACA​GA-3′ 5′-AAA​CAA​CTG​TAC​GGA​CAT​CAC​AGA​C-3′ 
Ifngr2 sgRNA 2 5′-TTC​AGT​ATC​TTG​TCC​ACT​AC-3′ 5′-CAC​CGT​TCA​GTA​TCT​TGT​CCA​CTA​C-3′ 
Complementary 5′-GTA​GTG​GAC​AAG​ATA​CTG​AA-3′ 5′-AAA​CGT​AGT​GGA​CAA​GAT​ACT​GAA​C-3′ 
Ifngr2 sgRNA 3 5′-CAC​TGG​TCT​TGG​GTC​ATT​GC-3′ 5′-CAC​CGC​ACT​GGT​CTT​GGG​TCA​TTG​C-3′ 
Complementary 5′-GCA​ATG​ACC​CAA​GAC​CAG​TG-3′ 5′-AAA​CGC​AAT​GAC​CCA​AGA​CCA​GTG​C-3′ 
Ciita sgRNA 1 5′-ATA​GAG​ATC​CCT​GTA​GAA​GC-3′ 5′-CAC​CGA​TAG​AGA​TCC​CTG​TAG​AAG​C-3′ 
Complementary 5′-GCT​TCT​ACA​GGG​ATC​TCT​AT-3′ 5′-AAA​CGC​TTC​TAC​AGG​GAT​CTC​TAT​C-3′ 
Ciita sgRNA 2 5′-CCA​GTT​CAG​CAA​GCT​GTT​GC-3′ 5′-CAC​CGC​CAG​TTC​AGC​AAG​CTG​TTG​C-3′ 
Complementary 5′-GCA​ACA​GCT​TGC​TGA​ACT​GG-3′ 5′-AAA​CGC​AAC​AGC​TTG​CTG​AAC​TGG​C-3′ 
Ciita sgRNA 3 5′-TAC​TGA​AGA​GGT​CCT​TGC​TC-3′ 5′-CAC​CGT​ACT​GAA​GAG​GTC​CTT​GCT​C-3′ 
Complementary 5′-GAG​CAA​GGA​CCT​CTT​CAG​TA-3′ 5′-AAA​CGA​GCA​AGG​ACC​TCT​TCA​GTA​C-3′ 
H2-Aa sgRNA1 5′-ATA​GCA​AGT​CAG​TCG​CAG​A-3′ 5′-CAC​CGA​TAG​CAA​GTC​AGT​CGC​AGA-3′ 
Complementary 5′-TCT​GCG​ACT​GAC​TTG​CTA​T-3′ 5′-AAA​CTC​TGC​GAC​TGA​CTT​GCT​ATC-3′ 
H2-Aa sgRNA2 5′-TAT​ACC​ATA​GGT​GCC​TAC​G-3′ 5′-CAC​CGT​ATA​CCA​TAG​GTG​CCT​ACG-3′ 
Complementary 5′-CGT​AGG​CAC​CTA​TGG​TAT​A-3′ 5′-AAA​CCG​TAG​GCA​CCT​ATG​GTA​TAC-3′ 
H2-Ab1 sgRNA1 5′-CGT​ATG​CGC​TGC​GTC​CCG​T-3′ 5′-CAC​CGC​GTA​TGC​GCT​GCG​TCC​CGT-3′ 
Complementary 5′-ACG​GGA​CGC​AGC​GCA​TAC​G-3′ 5′-AAA​CAC​GGG​ACG​CAG​CGC​ATA​CGC-3′ 
H2-Ab1 sgRNA2 5′-GCG​GCC​AGA​CGC​CGA​GTA​C-3′ 5′-CAC​CGG​CGG​CCA​GAC​GCC​GAG​TAC-3′ 
Complementary 5′-GTA​CTC​GGC​GTC​TGG​CCG​C-3′ 5′-AAA​CGT​ACT​CGG​CGT​CTG​GCC​GCC-3′ 
H2-Eb1 sgRNA1 5′-CAA​CGG​GAC​GCA​GCG​CGT​G-3′ 5′-CAC​CGC​AAC​GGG​ACG​CAG​CGC​GTG-3′ 
Complementary 5′-CAC​GCG​CTG​CGT​CCC​GTT​G-3′ 5′-AAA​CCA​CGC​GCT​GCG​TCC​CGT​TGC-3′ 
H2-Eb1 sgRNA2 5′-GAC​GTG​GGC​GAG​TTC​CGC​G-3′ 5′-CAC​CGG​ACG​TGG​GCG​AGT​TCC​GCG-3′ 
Complementary 5′-CGC​GGA​ACT​CGC​CCA​CGT​C-3′ 5′-AAA​CCG​CGG​AAC​TCG​CCC​ACG​TCC-3′ 
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