Motional mode, compartment size (L), DMACRO, and dwell lifetime (τ), characterizing the hop diffusions of Cy3-DOPE, TfR, and EGFR in apical and basal PMs of T24 cells
| . | Motional Mode (%) . | Cmpt. Size (L, nm)b . | DMACRO (µm2/s)b . | Residency Lifetime (τ, ms)b . | nc . | |
|---|---|---|---|---|---|---|
| . | Suppa . | Simpa . | Median Mean ± SEM Histogram Fig. No.d . | Median Mean ± SEM Histogram Fig. No. . | Mean ± SEM Histogram Fig. No. . | . |
| Cy3-DOPE (Apical PM) | 76 | 24 | 101 107 ± 6.2#1,#2 Fig. 6 C | 0.30 0.30 ± 0.021#3,#4 Fig. 6 E | 9.2 ± 0.34#5,#6 Fig. 6 D | 50 |
| Cy3-DOPE (Basal PM) | 74 | 26 | 107 107 ± 5.6#7,#8,N2 Fig. 6 C | 0.31 0.34 ± 0.022#9,N4 Fig. 6 E | 10 ± 0.61#10,N6 Fig. 6 D | 50 |
| TfR (Apical PM) | 80 | 20 | 103 107 ± 5.4#11,N1 Fig. 6 C | 0.11 0.13 ± 0.014#12,Y3 Fig. 6 E | 23 ± 1.5#13,Y5 Fig. 6 D | 50 |
| TfR (Basal PM; Outside FA) | 72 | 28 | 109 121 ± 6.9#14,N7,N11 Fig. 6 C | 0.12 0.14 ± 0.016Y9,N12 Fig. 6 E | 24 ± 1.6Y10,N13 Fig. 6 D | 57 |
| EGFR (Basal PM; Before stimulation) | 73 | 27 | 106 119 ± 6.5#15,N8,N14 Fig. 7 B | 0.19 0.21 ± 0.024#16 Fig. 7 D | 18 ± 1.2#17 Fig. 7 C | 41 |
| EGFR (Basal PM; 2.5–5 min after stimulation) | 85 | 15 | 106 110 ± 6.0N15 Fig. 7 B | 0.11 0.12 ± 0.019Y16 Fig. 7 D | 27 ± 1.5Y17 Fig. 7 C | 39 |
| . | Motional Mode (%) . | Cmpt. Size (L, nm)b . | DMACRO (µm2/s)b . | Residency Lifetime (τ, ms)b . | nc . | |
|---|---|---|---|---|---|---|
| . | Suppa . | Simpa . | Median Mean ± SEM Histogram Fig. No.d . | Median Mean ± SEM Histogram Fig. No. . | Mean ± SEM Histogram Fig. No. . | . |
| Cy3-DOPE (Apical PM) | 76 | 24 | 101 107 ± 6.2#1,#2 Fig. 6 C | 0.30 0.30 ± 0.021#3,#4 Fig. 6 E | 9.2 ± 0.34#5,#6 Fig. 6 D | 50 |
| Cy3-DOPE (Basal PM) | 74 | 26 | 107 107 ± 5.6#7,#8,N2 Fig. 6 C | 0.31 0.34 ± 0.022#9,N4 Fig. 6 E | 10 ± 0.61#10,N6 Fig. 6 D | 50 |
| TfR (Apical PM) | 80 | 20 | 103 107 ± 5.4#11,N1 Fig. 6 C | 0.11 0.13 ± 0.014#12,Y3 Fig. 6 E | 23 ± 1.5#13,Y5 Fig. 6 D | 50 |
| TfR (Basal PM; Outside FA) | 72 | 28 | 109 121 ± 6.9#14,N7,N11 Fig. 6 C | 0.12 0.14 ± 0.016Y9,N12 Fig. 6 E | 24 ± 1.6Y10,N13 Fig. 6 D | 57 |
| EGFR (Basal PM; Before stimulation) | 73 | 27 | 106 119 ± 6.5#15,N8,N14 Fig. 7 B | 0.19 0.21 ± 0.024#16 Fig. 7 D | 18 ± 1.2#17 Fig. 7 C | 41 |
| EGFR (Basal PM; 2.5–5 min after stimulation) | 85 | 15 | 106 110 ± 6.0N15 Fig. 7 B | 0.11 0.12 ± 0.019Y16 Fig. 7 D | 27 ± 1.5Y17 Fig. 7 C | 39 |
Supp = Suppressed diffusion. Simp = Simple-Brownian diffusion.
Cmpt. Size = Compartment size (L). L and DMACRO in the intact PM were estimated by the hop-diffusion fitting. Residency Lifetime (τ) = exponential decay lifetime (mean ± SEM; SEM was determined from the fitting error of the 68.3% confidence interval) within a compartment, based on the residency time distributions obtained by the TILD analysis (Fig. S5).
The number of inspected molecules.
Histogram. Fig. No. = The figure in which the histogram (distribution) is shown.
, Y, and N. The results of the statistical tests. The distributions selected as the basis for the comparisons are shown by the superscript, #. Different numbers (1–17) indicate different bases for the Brunner-Munzel test (1–4, 7–9, 11 12, 14–16, 15, and 16) and the log-rank test (5, 6, 10, 13, and 17). The superscript Y (or N) with numbers indicates that the distribution is (or is not) significantly different from the base distribution indicated by the number after #, with P values smaller (or greater) than 0.05 as shown below.
N1: P = 0.85, N2: P = 0.70, Y3: P = 4.3 × 10−14, N4: P = 0.20, Y5: P = 1.2 × 10−29, N6: P = 0.27, N7: P = 0.40, N8: P = 0.31, Y9: P = 2.2 × 10−16, Y10: P = 6.0 × 10−31, N11: P = 0.26, N12: P = 0.81, N13: P = 0.17, N14: P = 0.81, N15: P = 0.43, Y16: P = 8.7 × 10−4, Y17: P = 0.044.