Gating parameters of T449A/A471C, T449A/L472C, and T449A/P473C//T449 constructs
| . | Voltage-dependence of steady-state activation . | Voltage-dependence of steady-state inactivation . | Inactivation kinetics . | RFI . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| V1/2 (mV) | s (mV) | n | V1/2 (mV) | s (mV) | n | τi (ms) | n | τrec (s) | n | |
| T449A/A471C | 0.3 ± 2.9 | 22.3 ± 3.2 | 6 | −52.8 ± 2.2 | −7.3 ± 1.2 | 5 | 16.3 ± 1.9 | 23 | 9.4 ± 0.8 | 5 |
| T449A/L472C | −26.0 ± 2.0 | 12.1 ± 1.5 | 6 | −72.5 ± 3.5 | −12.6 ± 3.6 | 5 | 26.6 ± 2.3 | 24 | 4.4 ± 0.7 | 5 |
| T449A/P473C//T449 | −19.0 ± 7.2 | 17.5 ± 1.9 | 6 | −58.2 ± 4.4 | −6.3 ± 2.1 | 4 | τi,f = 69.1 ± 6.6 τi,s = 595.0 ± 51.0 | 25 | 1.8 ± 0.2 | 5 |
| T449A (Szanto et al., 2021) | −19.8 ± 1.5 | 14.7 ± 0.8 | 7 | −67.8 ± 3.6 | −6.4 ± 0.8 | 7 | 103.0 ± 8.0 | 9 | 3.7 ± 0.3 | 8 |
| Shaker-IR (Martinez-Morales et al., 2014) | −28.3 ± 2.8 | 4.9 ± 0.4 | n.a. | n.a. | ||||||
| . | Voltage-dependence of steady-state activation . | Voltage-dependence of steady-state inactivation . | Inactivation kinetics . | RFI . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| V1/2 (mV) | s (mV) | n | V1/2 (mV) | s (mV) | n | τi (ms) | n | τrec (s) | n | |
| T449A/A471C | 0.3 ± 2.9 | 22.3 ± 3.2 | 6 | −52.8 ± 2.2 | −7.3 ± 1.2 | 5 | 16.3 ± 1.9 | 23 | 9.4 ± 0.8 | 5 |
| T449A/L472C | −26.0 ± 2.0 | 12.1 ± 1.5 | 6 | −72.5 ± 3.5 | −12.6 ± 3.6 | 5 | 26.6 ± 2.3 | 24 | 4.4 ± 0.7 | 5 |
| T449A/P473C//T449 | −19.0 ± 7.2 | 17.5 ± 1.9 | 6 | −58.2 ± 4.4 | −6.3 ± 2.1 | 4 | τi,f = 69.1 ± 6.6 τi,s = 595.0 ± 51.0 | 25 | 1.8 ± 0.2 | 5 |
| T449A (Szanto et al., 2021) | −19.8 ± 1.5 | 14.7 ± 0.8 | 7 | −67.8 ± 3.6 | −6.4 ± 0.8 | 7 | 103.0 ± 8.0 | 9 | 3.7 ± 0.3 | 8 |
| Shaker-IR (Martinez-Morales et al., 2014) | −28.3 ± 2.8 | 4.9 ± 0.4 | n.a. | n.a. | ||||||
V1/2 and s values were obtained by the Boltzmann function to the data. The inactivation time constant (τi) obtained from single-exponential fits or the time constants for the fast (τi,f) and slow (τi,s) components of the decay, obtained from fitting the sum of two exponential terms to the data points, are indicated in the “Inactivation kinetics” column. τrec is the time constant for recovery from inactivation (RFI), n indicates the number of independent experiments. See Materials and methods for the equations used for fitting. Data for the inactivation removed T449A and Shaker are from the references indicated in the parentheses; n.a., not applicable.