Scores for intermolecular arrangements of CGN and CGNL1 with NM2A, NM2B, and NM2C
| . | Parallel arrangement . | Antiparallel arrangement . | ||||
|---|---|---|---|---|---|---|
| Stagger | Score | Overlap | Stagger | Score | Overlap | |
| CGN-NM2A | ND | ND | ND | 421 | 0.2508 | CC50 |
| CGN-NM2B | 440 | 0.2599 | NS | 421 | 0.2508 | CC50 |
| CGN-NM2C | 441 | 0.2364 | NS | 421 | 0.2606 | CC50 |
| CGNL1-NM2A | 267 | NS | CN109 | 304 | 0.2373 | CC72 |
| CGNL1-NM2B | 330 | 0.2420 | CN46 | 304 | 0.2442 | CC72 |
| CGNL1-NM2C | 326 | NS | CN50 | 304 | 0.2716 | CC72 |
| . | Parallel arrangement . | Antiparallel arrangement . | ||||
|---|---|---|---|---|---|---|
| Stagger | Score | Overlap | Stagger | Score | Overlap | |
| CGN-NM2A | ND | ND | ND | 421 | 0.2508 | CC50 |
| CGN-NM2B | 440 | 0.2599 | NS | 421 | 0.2508 | CC50 |
| CGN-NM2C | 441 | 0.2364 | NS | 421 | 0.2606 | CC50 |
| CGNL1-NM2A | 267 | NS | CN109 | 304 | 0.2373 | CC72 |
| CGNL1-NM2B | 330 | 0.2420 | CN46 | 304 | 0.2442 | CC72 |
| CGNL1-NM2C | 326 | NS | CN50 | 304 | 0.2716 | CC72 |
Table shows normalized scores (>0.23) for the total number of potential apolar and ionic interactions are shown as a function of axial stagger and relative orientation between interacting molecules. Scores <0.23 or short overlaps (<32) were NS. ND indicates no score. Axial stagger is designated in terms of the number of residues in a coiled-coil conformation. CC50, for example, indicates molecules overlapping by 50 residues over the C-terminal portion of each of their coiled-coil domains, and CN indicates a C-terminal overlap of CGN or CGNL1 with an N-terminal region of the rod of NM2A, NM2B, or NM2C. Scores were calculated as described in Hulmes et al. (1973); Parry et al. (1977); Fraser et al. (1986).