Table 2.

Concentration–response data for glycine at NMDA receptor subtypes

[L-689,560] (µM)EC50 (µM)Hill slopen
GluN1/2A 0a 0.87 ± 0.01 1.3 6 
0.1a 11 ± 1 1.7 3 
0.3a 27 ± 2 1.8 6 
1a 80 ± 7 1.6 6 
3a 267 ± 42 1.4 6 
GluN1/3A 0b 14 ± 1 1.9 13 
0c 19 ± 1 1.9 18 
1 714 ± 71 1.8 8 
10 802 ± 60 1.8 14 
GluN1/3B 0b 19 ± 1 1.8 20 
0c 26 ± 1 1.6 17 
1 351 ± 11 1.6 8 
10 410 ± 9 1.6 14 
GluN1FA+TL/3A 0 53 ± 3 1.5 12 
0.03 58 ± 5 1.5 10 
0.1 92 ± 8 1.6 10 
0.3 164 ± 14 1.6 6 
1 269 ± 12 1.6 8 
3 331 ± 15 1.5 6 
10 356 ± 7 1.6 6 
GluN1FH+RK/3A 0 162 ± 8 1.4 12 
GluN1FA+TL/3B 0 95 ± 7 1.4 13 
10 102 ± 3 1.3 21 
GluN1FH+RK/3B 0 270 ± 3 1.4 16 
[L-689,560] (µM)EC50 (µM)Hill slopen
GluN1/2A 0a 0.87 ± 0.01 1.3 6 
0.1a 11 ± 1 1.7 3 
0.3a 27 ± 2 1.8 6 
1a 80 ± 7 1.6 6 
3a 267 ± 42 1.4 6 
GluN1/3A 0b 14 ± 1 1.9 13 
0c 19 ± 1 1.9 18 
1 714 ± 71 1.8 8 
10 802 ± 60 1.8 14 
GluN1/3B 0b 19 ± 1 1.8 20 
0c 26 ± 1 1.6 17 
1 351 ± 11 1.6 8 
10 410 ± 9 1.6 14 
GluN1FA+TL/3A 0 53 ± 3 1.5 12 
0.03 58 ± 5 1.5 10 
0.1 92 ± 8 1.6 10 
0.3 164 ± 14 1.6 6 
1 269 ± 12 1.6 8 
3 331 ± 15 1.5 6 
10 356 ± 7 1.6 6 
GluN1FH+RK/3A 0 162 ± 8 1.4 12 
GluN1FA+TL/3B 0 95 ± 7 1.4 13 
10 102 ± 3 1.3 21 
GluN1FH+RK/3B 0 270 ± 3 1.4 16 

Glycine concentration–response data were recorded using two-electrode voltage-clamp electrophysiology at recombinant NMDA receptors expressed in Xenopus oocytes. Responses were measured in the continuous presence of L-689,560 at the indicated concentrations. Data are presented as mean ± SEM of values from individual oocytes, and n is the number of oocytes used to generate the data.

a

Measured in the continuous presence of 300 µM glutamate.

b

Measured in the continuous presence of 1 µM CGP-78608.

c

Measured in the continuous presence of 10 µM CGP-78608.

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