Steady-state and dynamic contractile parameters measured in chemically skinned myocardial preparations from cMyBPC−/− mice treated with saline, AAV9-FL, and AAV9-C3C10
| . | Saline . | C3C10 . | FL . |
|---|---|---|---|
| Fmin (mN/mm2) | 0.71 ± 0.08 | 0.60 ± 0.09 | 0.55 ± 0.09 |
| Fmax (mN/mm2) | 11.35 ± 0.72 | 10.73 ± 0.74 | 8.48 ± 0.74 |
| nH | 3.5 ± 0.3 | 3.1 ± 0.3 | 3.8 ± 0.3 |
| pCa50 | 5.97 ± 0.02 | 5.93 ± 0.01 | 5.94 ± 0.02 |
| krel (s−1) | 945 ± 147a, P = 0.0005 | 903 ± 168a, P = 0.0013 | 590 ± 115b, P = 0.0005 |
| kdf (s−1) | 20 ± 3a, P = 0.0001 | 13 ± 4b, P = 0.0018 | 10 ± 2b, P = 0.0001 |
| . | Saline . | C3C10 . | FL . |
|---|---|---|---|
| Fmin (mN/mm2) | 0.71 ± 0.08 | 0.60 ± 0.09 | 0.55 ± 0.09 |
| Fmax (mN/mm2) | 11.35 ± 0.72 | 10.73 ± 0.74 | 8.48 ± 0.74 |
| nH | 3.5 ± 0.3 | 3.1 ± 0.3 | 3.8 ± 0.3 |
| pCa50 | 5.97 ± 0.02 | 5.93 ± 0.01 | 5.94 ± 0.02 |
| krel (s−1) | 945 ± 147a, P = 0.0005 | 903 ± 168a, P = 0.0013 | 590 ± 115b, P = 0.0005 |
| kdf (s−1) | 20 ± 3a, P = 0.0001 | 13 ± 4b, P = 0.0018 | 10 ± 2b, P = 0.0001 |
Values are expressed as mean ± SEM. n = 4 hearts with three preparations per heart, analyzed by one-way repeated measures ANOVA with post hoc Tukey’s multiple comparisons test. Fmin, Ca2+-independent force analyzed at pCa9.0; Fmax, Ca2+-activated maximal force measured at pCa 4.5; nH, Hill coefficient of the force–pCa relationship; pCa50, pCa required for the generation of half-maximal force; krel, rate of cross-bridge detachment; kdf, rate of cross-bridge recruitment.
Significantly different compared to FL.
Significantly different compared to saline.