Table 1.

n indicates number of biological replicates, i.e., number of mice

Saline (n = 9)C3C10 (n = 8)FL (n = 13)
Age (wk) 6.32 ± 0.08 6.34 ± 0.05 6.25 ± 0.03 
HR (BPM) 451 ± 2 448 ± 2 449 ± 2 
BW (g) 17.44 ± 0.38 17.75 ± 0.59 16.31 ± 0.52 
LVM (mg) 184.92 ± 5.93a, P < 0.000001 141.35 ± 6.05
aP < 0.000001,
bP < 0.000001 
105.57 ± 3.77, P < 0.000001 
LVM/BW (mg/g) 10.61 ± 0.31a, P < 0.000001 7.97 ± 0.23
aP < 0.000001,
bP < 0.000001 
6.48 ± 0.10b, P < 0.000001 
LVAW:d (mm) 1.19 ± 0.04a, P = 0.0046 1.065 ± 0.07 0.998 ± 0.03b, P = 0.0046 
LVPW:d (mm) 1.35 ± 0.08a, P = 0.00010 1.12 ± 0.05b, P = 0.015 1.02 ± 0.03b, P = 0.00010 
Diameter:d (mm) 4.05 ± 0.13a, P = 0.00024 3.93 ± 0.06a, P = 0.0056 3.50 ± 0.07b, P = 0.00024 
FS (%) 15.97 ± 0.54a, P < 0.000001 21.15 ± 0.73b, P = 0.00006 22.91 ± 0.64b, P < 0.000001 
EF (%) 34.02 ± 1.01a, P < 0.000001 43.51 ± 1.27b, P = 0.000033 46.9 ± 1.12b, P < 0.000001 
GLS (%) −8.52 ± 0.65a, P < 0.000001 −11.07 ± 0.26
aP = 0.019,
bP = 0.0052 
−13.06 ± 0.43b, P < 0.000001 
AET (ms) 32.18 ± 1.08a, P = 0.000023 35.00 ± 0.96a, P = 0.0014 41.62 ± 1.08b, P = 0.000023 
rLSR (s−13.51 ± 0.34a, P = 0.00070 4.32 ± 0.52a, P = 0.019 6.35 ± 0.52b, P = 0.00070 
Saline (n = 9)C3C10 (n = 8)FL (n = 13)
Age (wk) 6.32 ± 0.08 6.34 ± 0.05 6.25 ± 0.03 
HR (BPM) 451 ± 2 448 ± 2 449 ± 2 
BW (g) 17.44 ± 0.38 17.75 ± 0.59 16.31 ± 0.52 
LVM (mg) 184.92 ± 5.93a, P < 0.000001 141.35 ± 6.05
aP < 0.000001,
bP < 0.000001 
105.57 ± 3.77, P < 0.000001 
LVM/BW (mg/g) 10.61 ± 0.31a, P < 0.000001 7.97 ± 0.23
aP < 0.000001,
bP < 0.000001 
6.48 ± 0.10b, P < 0.000001 
LVAW:d (mm) 1.19 ± 0.04a, P = 0.0046 1.065 ± 0.07 0.998 ± 0.03b, P = 0.0046 
LVPW:d (mm) 1.35 ± 0.08a, P = 0.00010 1.12 ± 0.05b, P = 0.015 1.02 ± 0.03b, P = 0.00010 
Diameter:d (mm) 4.05 ± 0.13a, P = 0.00024 3.93 ± 0.06a, P = 0.0056 3.50 ± 0.07b, P = 0.00024 
FS (%) 15.97 ± 0.54a, P < 0.000001 21.15 ± 0.73b, P = 0.00006 22.91 ± 0.64b, P < 0.000001 
EF (%) 34.02 ± 1.01a, P < 0.000001 43.51 ± 1.27b, P = 0.000033 46.9 ± 1.12b, P < 0.000001 
GLS (%) −8.52 ± 0.65a, P < 0.000001 −11.07 ± 0.26
aP = 0.019,
bP = 0.0052 
−13.06 ± 0.43b, P < 0.000001 
AET (ms) 32.18 ± 1.08a, P = 0.000023 35.00 ± 0.96a, P = 0.0014 41.62 ± 1.08b, P = 0.000023 
rLSR (s−13.51 ± 0.34a, P = 0.00070 4.32 ± 0.52a, P = 0.019 6.35 ± 0.52b, P = 0.00070 

Values are expressed as mean ± SEM., analyzed by one-way ANOVA with post hoc Tukey’s multiple comparisons test. HR, heart rate; BW, body weight; LVM, left ventricle mass; LVAW:d, left ventricle anterior wall thickness in diastole; LVPW:d, left ventricle posterior wall thickness in diastole; Diameter:d, left ventricle diameter in diastole; FS, fractional shortening; EF, ejection fraction; GLS, global longitudinal strain; AET, aortic ejection time; rLSR, reverse longitudinal strain rate.

a

Significantly different compared to FL.

b

Significantly different compared to saline.

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