Late current parameters for NaV1.4 channels
| Condition . | ILATE, % . | τ, ms . |
|---|---|---|
| CRY2-PJ | ||
| Control | 0.24 ± 0.02 | 4.7 ± 0.3 |
| BL | 1.95 ± 0.2 (P = 0.0001) | 8.9 ± 0.8 (P = 0.0014) |
| Control + diC8PI(4,5)P2 | 0.37 ± 0.1 | 4.7 ± 0.2 |
| BL + diC8PI(4,5)P2 | 0.38 ± 0.03 | 5.1 ± 0.3 |
| CRY2-5POCRL | ||
| Control | 0.24 ± 0.02 | 6.3 ± 0.2 |
| BL | 1.93 ± 0.07 (P = 0.0001) | 10.0 ± 0.4 (P = 0.014) |
| Control + diC8PI(4,5)P2 | 0.26 ± 0.02 | 6.0 ± 0.3 |
| BL + diC8PI(4,5)P2 | 0.27 ± 0.02 | 7.4 ± 0.6 |
| Condition . | ILATE, % . | τ, ms . |
|---|---|---|
| CRY2-PJ | ||
| Control | 0.24 ± 0.02 | 4.7 ± 0.3 |
| BL | 1.95 ± 0.2 (P = 0.0001) | 8.9 ± 0.8 (P = 0.0014) |
| Control + diC8PI(4,5)P2 | 0.37 ± 0.1 | 4.7 ± 0.2 |
| BL + diC8PI(4,5)P2 | 0.38 ± 0.03 | 5.1 ± 0.3 |
| CRY2-5POCRL | ||
| Control | 0.24 ± 0.02 | 6.3 ± 0.2 |
| BL | 1.93 ± 0.07 (P = 0.0001) | 10.0 ± 0.4 (P = 0.014) |
| Control + diC8PI(4,5)P2 | 0.26 ± 0.02 | 6.0 ± 0.3 |
| BL + diC8PI(4,5)P2 | 0.27 ± 0.02 | 7.4 ± 0.6 |
ILATE is the percentage of the peak Na+ current (IPEAK) measured as an average between 100 and 150 ms after the start of a depolarizing pulse to −20 mV, as described in the Materials and methods. The time-constant for fast inactivation, τ, reports the rate of transition from open (IPEAK) to ILATE. Where indicated, diC8PI(4,5)P2 was included in the recording pipette at 200 µM. The data are from graphs plotted in Fig. 6. The mean values are from 10–15 cells per group obtained from three independent biological replicates of the experiment. Statistical significance was determined using a Student’s paired t test.