Table 2. Voltage-dependent activation... | Rockefeller University Press

Table 2.

Voltage-dependent activation and inactivation parameters for Na_V1.4 channels

Condition	G–V		SSI
	V½_act, mV	K_Act, mV	V½_SSI, mV	K_SSI, mV
CRY2-PJ
Control	−38 ± 2	5.0 ± 0.5	−58 ± 1	−4.4 ± 0.5
BL	−48 ± 2 (P = 0.007)	5.1 ± 0.5	−58 ± 4	−7.0 ± 0.6 (P = 0.022)
Control + diC₈PI(4,5)P₂	−33 ± 3	4.0 ± 0.7	−55 ± 4	−4.2 ± 1.0
BL + diC₈PI(4,5)P₂	−33 ± 4	5.0 ± 1.0	−54 ± 2	−5.1 ± 0.5
CRY2-5P_OCRL
Control	−37 ± 3	5.4 ± 0.4	−57 ± 3	−4.7 ± 1
BL	−44 ± 3 (P = 0.009)	5.1 ± 0.7	−58 ± 5	−6.0 ± 0.7
Control + diC₈PI(4,5)P₂	−32 ± 1	5.0 ± 0.2	−58 ± 2	−5.0 ± 2
BL + diC₈PI(4,5)P₂	−32 ± 1	5.2 ± 0.3	−57 ± 1	−5.1 ± 1

Condition	G–V		SSI
	V½_act, mV	K_Act, mV	V½_SSI, mV	K_SSI, mV
CRY2-PJ
Control	−38 ± 2	5.0 ± 0.5	−58 ± 1	−4.4 ± 0.5
BL	−48 ± 2 (P = 0.007)	5.1 ± 0.5	−58 ± 4	−7.0 ± 0.6 (P = 0.022)
Control + diC₈PI(4,5)P₂	−33 ± 3	4.0 ± 0.7	−55 ± 4	−4.2 ± 1.0
BL + diC₈PI(4,5)P₂	−33 ± 4	5.0 ± 1.0	−54 ± 2	−5.1 ± 0.5
CRY2-5P_OCRL
Control	−37 ± 3	5.4 ± 0.4	−57 ± 3	−4.7 ± 1
BL	−44 ± 3 (P = 0.009)	5.1 ± 0.7	−58 ± 5	−6.0 ± 0.7
Control + diC₈PI(4,5)P₂	−32 ± 1	5.0 ± 0.2	−58 ± 2	−5.0 ± 2
BL + diC₈PI(4,5)P₂	−32 ± 1	5.2 ± 0.3	−57 ± 1	−5.1 ± 1

Mean V½ values (± SEM) are determined from Boltzmann function fit to voltage-dependent activation and SSI studies for Na_V1.4 channels, as described in Figs. 2 and 4. V½_act and K_act are determined from G–V relationships calculated as described in the Materials and methods. V½_SSI and K_SSI are determined from Boltzmann fits to the SSI relationships of Na_V1.4 channels. Where indicated, 200 µM diC₈PI(4,5)P₂ was included in the recording pipette. The mean values are from 12–18 cells per group obtained from three independent biological replicates of the experiment. Statistical significance was determined using a Student’s paired t test.

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