NaV1.4 channel current-densities before and after dephosphorylation of PI(4,5)P2
| Condition IPEAK, pA/pF . | −60 mV . | −40 mV . | −20 mV . |
|---|---|---|---|
| CRY2-PJ | |||
| Control | −10 ± 1 | −262 ± 26 | −280 ± 28 |
| BL | −39 ± 8 | −348 ± 35 | −268 ± 32 |
| (P = 0.007) | (P = 0.028) | ||
| Control + diC8PI(4,5)P2 | −19 ± 2 | −194 ± 24 | −264 ± 18 |
| BL + diC8PI(4,5)P2 | −23 ± 3 | −209 ± 22 | −272 ± 24 |
| CRY2-5POCRL | |||
| Control | −10 ± 4 | −189 ± 24 | −262 ± 24 |
| BL | −37 ± 4 | −305 ± 15 | −247 ± 20 |
| (P = 0.012) | (P = 0.031) | ||
| Control + diC8PI(4,5)P2 | −23 ± 6 | −186 ± 28 | −274 ± 32 |
| BL + diC8PI(4,5)P2 | −19 ± 4 | −202 ± 21 | −268 ± 25 |
| Condition IPEAK, pA/pF . | −60 mV . | −40 mV . | −20 mV . |
|---|---|---|---|
| CRY2-PJ | |||
| Control | −10 ± 1 | −262 ± 26 | −280 ± 28 |
| BL | −39 ± 8 | −348 ± 35 | −268 ± 32 |
| (P = 0.007) | (P = 0.028) | ||
| Control + diC8PI(4,5)P2 | −19 ± 2 | −194 ± 24 | −264 ± 18 |
| BL + diC8PI(4,5)P2 | −23 ± 3 | −209 ± 22 | −272 ± 24 |
| CRY2-5POCRL | |||
| Control | −10 ± 4 | −189 ± 24 | −262 ± 24 |
| BL | −37 ± 4 | −305 ± 15 | −247 ± 20 |
| (P = 0.012) | (P = 0.031) | ||
| Control + diC8PI(4,5)P2 | −23 ± 6 | −186 ± 28 | −274 ± 32 |
| BL + diC8PI(4,5)P2 | −19 ± 4 | −202 ± 21 | −268 ± 25 |
Data are mean current-densities (± SEM) obtained from whole-cell patch-clamp recordings, using the I–V relationship described in the Materials and methods and shown in Figs. 1 and 3. 200 µM diC8PI(4,5)P2 was included in the patch pipette when indicated. The mean values are from 12–18 cells per group obtained from three independent biological replicates of the experiment. Statistical significance was determined using a Student’s paired t test.