Table 3.

Yeast strains used in this study

StrainGenotypeSource
W303 Mat a ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 can1-100 R. Rothstein 
JC470 Mat a W303 with RAD5+ J. Cobb 
JC471 Mat alpha W303 with RAD5+ J. Cobb 
JC1822 JC471 with RAP1-GFP::LEU2 Moradi-Fard et al., 2016  
JC2494 JC471 with 8HIS-Smt3::TRP1 RAP1-13MYC:HIS This study 
JC3737 JC470 with sir4Δ::HIS3 Moradi-Fard et al., 2016  
JC3786 JC470 with sir2Δ::TRP1 This study 
JC4311 JC470 with sir3Δ::HIS3 This study 
JC4263 JC471 with nup49::NUP49-GFP (Moradi-Fard et al., 2021) 
JC4438 JC471 with SIR4-GFP::KanMX6 TelVII::URA3 Gasser lab (GA2140) 
JC4881 JC470 with RAP1-13MYC::HIS3
Cross of JC2494 × JC470 
This study 
JC5046 JC470 with SIR4-GFP::KanMX6
Cross of JC4438 × JC470 
This study 
JC5047 JC470 with SIR4-GFP::KanMX6 HDEL-DsRed::LEU2 This study 
AT126 Mat a ade2-1::ADE2 can1-100 his3-11,15 ura3-1 leu2-3,112 trp1-1 rad5- Ruault et al. 2011  
AT1038 AT126 Mat alpha with hmr∆::HPH sir2∆::KanMX Taddei Lab 
AT2804 AT126 Mat alpha with hmr∆::HPH Taddei Lab 
GA1275 W303 with SIR4-13MYC::KanMX6 Perrod et al., 2001  
GA1459 MATa ade2-1 can1-100 his3-11,15::GFP-LacI-HIS3 trp1-1 ura3-1 leu2-3,112 nup49::NUP49-GFP-URA3 TELVI-R::lacO-lexA-TRP1
Kind gift of Dr. Susan Gasser 
Hediger et al., 2002  
GA1986 MATa ade2-1 can1-100 his3-11,15::GFP-LacI-HIS3 trp1-1 ura3-1 leu2-3,112 nup49::NUP49-GFP-URA3 TELVIII-L::lacO-lexA-TRP1
Kind gift of Dr. Susan Gasser 
Hediger et al., 2002​ 
CMY204 W303 with gpt2Δ::TRP1 Zaremberg and McMaster, 2002  
VZY452 JC470 with DsRed-HDEL::LEU2 This study 
KM014 W303 with rap1::LEU2 [pRS313-RAP1] Yeast genome resource Centrea 
KM015 W303 with rap1::LEU2 [pRS313-rap1-12] Yeast genome resource Centrea 
KM016 W303 with rap1::LEU2 [pRS313-rap1-17] Yeast genome resource Centrea 
BY4741 MATa his3Δ leu2Δ met15Δ ura3Δ Euroscarf 
mga2∆ BY4741 with mga2Δ::KanMX4 Euroscarf 
spt23∆ BY4741 with spt23Δ::KanMX4 Euroscarf 
VZY397 BY4741 with DsRed-HDEL::LEU2 This study 
VZY453 BY4741 with sir4Δ::KanMX4 DsRed-HDEL::LEU2 This study 
StrainGenotypeSource
W303 Mat a ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 can1-100 R. Rothstein 
JC470 Mat a W303 with RAD5+ J. Cobb 
JC471 Mat alpha W303 with RAD5+ J. Cobb 
JC1822 JC471 with RAP1-GFP::LEU2 Moradi-Fard et al., 2016  
JC2494 JC471 with 8HIS-Smt3::TRP1 RAP1-13MYC:HIS This study 
JC3737 JC470 with sir4Δ::HIS3 Moradi-Fard et al., 2016  
JC3786 JC470 with sir2Δ::TRP1 This study 
JC4311 JC470 with sir3Δ::HIS3 This study 
JC4263 JC471 with nup49::NUP49-GFP (Moradi-Fard et al., 2021) 
JC4438 JC471 with SIR4-GFP::KanMX6 TelVII::URA3 Gasser lab (GA2140) 
JC4881 JC470 with RAP1-13MYC::HIS3
Cross of JC2494 × JC470 
This study 
JC5046 JC470 with SIR4-GFP::KanMX6
Cross of JC4438 × JC470 
This study 
JC5047 JC470 with SIR4-GFP::KanMX6 HDEL-DsRed::LEU2 This study 
AT126 Mat a ade2-1::ADE2 can1-100 his3-11,15 ura3-1 leu2-3,112 trp1-1 rad5- Ruault et al. 2011  
AT1038 AT126 Mat alpha with hmr∆::HPH sir2∆::KanMX Taddei Lab 
AT2804 AT126 Mat alpha with hmr∆::HPH Taddei Lab 
GA1275 W303 with SIR4-13MYC::KanMX6 Perrod et al., 2001  
GA1459 MATa ade2-1 can1-100 his3-11,15::GFP-LacI-HIS3 trp1-1 ura3-1 leu2-3,112 nup49::NUP49-GFP-URA3 TELVI-R::lacO-lexA-TRP1
Kind gift of Dr. Susan Gasser 
Hediger et al., 2002  
GA1986 MATa ade2-1 can1-100 his3-11,15::GFP-LacI-HIS3 trp1-1 ura3-1 leu2-3,112 nup49::NUP49-GFP-URA3 TELVIII-L::lacO-lexA-TRP1
Kind gift of Dr. Susan Gasser 
Hediger et al., 2002​ 
CMY204 W303 with gpt2Δ::TRP1 Zaremberg and McMaster, 2002  
VZY452 JC470 with DsRed-HDEL::LEU2 This study 
KM014 W303 with rap1::LEU2 [pRS313-RAP1] Yeast genome resource Centrea 
KM015 W303 with rap1::LEU2 [pRS313-rap1-12] Yeast genome resource Centrea 
KM016 W303 with rap1::LEU2 [pRS313-rap1-17] Yeast genome resource Centrea 
BY4741 MATa his3Δ leu2Δ met15Δ ura3Δ Euroscarf 
mga2∆ BY4741 with mga2Δ::KanMX4 Euroscarf 
spt23∆ BY4741 with spt23Δ::KanMX4 Euroscarf 
VZY397 BY4741 with DsRed-HDEL::LEU2 This study 
VZY453 BY4741 with sir4Δ::KanMX4 DsRed-HDEL::LEU2 This study 
a

Strain provided by the NBRP, Japan.

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