Primers used to quantify cadm3 sgRNA efficiency
| Primer name . | Sequence (5′ to 3′) . |
|---|---|
| cadm3-sg-test-F-1 | 5′-TGATGATGGGGACGAAGCAT-3′ |
| cadm3-sg-test-R-1 | 5′-TGATAAGGGGGAACCAGCCA-3′ |
| cadm3-sg-test-F-2 | 5′-TGCTGTCGTCGTTTTGCTTG-3′ |
| cadm3-sg-test-R-2 | 5′-ACTGACAGCTCCCACTCTGA-3′ |
| Primer name . | Sequence (5′ to 3′) . |
|---|---|
| cadm3-sg-test-F-1 | 5′-TGATGATGGGGACGAAGCAT-3′ |
| cadm3-sg-test-R-1 | 5′-TGATAAGGGGGAACCAGCCA-3′ |
| cadm3-sg-test-F-2 | 5′-TGCTGTCGTCGTTTTGCTTG-3′ |
| cadm3-sg-test-R-2 | 5′-ACTGACAGCTCCCACTCTGA-3′ |