Table 3. Characteristics of human... | Rockefeller University Press

Table 3.

Characteristics of human failing permeabilized myocytes before and after 3 h cTn exchange containing cTnI S23/24DD

Human samples	Myofibrillar preparations
Human samples	Before	After S2223DD
n	6
Fiber length (µm)	148 ± 10
Fiber width (µm)	25 ± 3
Resting sarcomere length (µm)	2.25 ± 0.02
Passive force (µN)	0.52 ± 0.13	0.44 ± 0.16
Passive tension (kN/m²)	1.48 ± 0.21	0.96 ± 0.23
Max force (µN)	15.1 ± 4.6	12.6 ± 3.5^{^a}
Max tension (kN/m²)	39.9 ± 3.4	34.9 ± 3.2^{^a}
k_tr @ pCa 4.5 (s⁻¹)	0.712 ± 0.08	0.595 ± 0.06^{^a}
pCa for half-maximal force	6.08 ± 0.03	5.88 ± 0.03^{^a}
Relative force (P_O/P_4.5)	0.49 ± 0.04	0.55 ± 0.03
a/P_O	0.16 ± 0.02	0.23 ± 0.05
F_opt (P/P_O)	0.266 ± 0.01	0.291 ± 0.02
V_opt (ML/s)	0.104 ± 0.010	0.117 ± 0.013
V_max	0.39 ± 0.04	0.42 ± 0.08
PNPO (P/P_O*ML/s)	0.027 ± 0.002	0.033 ± 0.003^{^a}
Submax k_tr	0.552 ± 0.10	0.772 ± 0.11^{^a}

Human samples	Myofibrillar preparations
Human samples	Before	After S2223DD
n	6
Fiber length (µm)	148 ± 10
Fiber width (µm)	25 ± 3
Resting sarcomere length (µm)	2.25 ± 0.02
Passive force (µN)	0.52 ± 0.13	0.44 ± 0.16
Passive tension (kN/m²)	1.48 ± 0.21	0.96 ± 0.23
Max force (µN)	15.1 ± 4.6	12.6 ± 3.5^{^a}
Max tension (kN/m²)	39.9 ± 3.4	34.9 ± 3.2^{^a}
k_tr @ pCa 4.5 (s⁻¹)	0.712 ± 0.08	0.595 ± 0.06^{^a}
pCa for half-maximal force	6.08 ± 0.03	5.88 ± 0.03^{^a}
Relative force (P_O/P_4.5)	0.49 ± 0.04	0.55 ± 0.03
a/P_O	0.16 ± 0.02	0.23 ± 0.05
F_opt (P/P_O)	0.266 ± 0.01	0.291 ± 0.02
V_opt (ML/s)	0.104 ± 0.010	0.117 ± 0.013
V_max	0.39 ± 0.04	0.42 ± 0.08
PNPO (P/P_O*ML/s)	0.027 ± 0.002	0.033 ± 0.003^{^a}
Submax k_tr	0.552 ± 0.10	0.772 ± 0.11^{^a}

Mechanical properties were tested before and after cTn exchange and compared via paired t test analysis. Values are mean ± SEM.

P ≤ 0.05. n equals the number of human hearts (biological replicates), with one cardiac myocyte preparation per heart.