Table 1. Characteristics of human... | Rockefeller University Press

Table 1.

Characteristics of human failing permeabilized myocytes before and after PKA

Human samples	Myofibrillar preparations
Human samples	Before	After PKA
n	8
Fiber length (µm)	153 ± 8
Fiber width (µm)	27 ± 2
Resting sarcomere length (µm)	2.20 ± 0.02
Passive force (µN)	0.53 ± 0.08	0.58 ± 0.08
Passive tension (kN/m²)	1.32 ± 0.18	1.39 ± 0.14
Max force (µN)	14.0 ± 2.3	14.7 ± 2.2
Max tension (kN/m²)	30.9 ± 3.5	36.6 ± 4.6^{^a}
k_tr @ pCa 4.5 (s⁻¹)	0.515 ± 0.04	0.666 ± 0.04^{^a}
pCa for half-maximal force	6.06 ± 0.03	5.96 ± 0.03
Relative force (P_O/P_4.5)	0.50 ± 0.04	0.52 ± 0.04
a/P_O	0.16 ± 0.04	0.20 ± 0.07
F_opt (P/P_O)	0.25 ± 0.02	0.27 ± 0.02
V_opt (ML/s)	0.100 ± 0.012	0.117 ± 0.009
V_max	0.45 ± 0.09	0.47 ± 0.07
PNPO (P/P_O*ML/s)	0.024 ± 0.002	0.031 ± 0.002^{^a}
Submax k_tr	0.456 ± 0.05	0.468 ± 0.05

Humansamples	Myofibrillar preparations
Humansamples	Before	After PKA
n	8
Fiber length (µm)	153 ± 8
Fiber width (µm)	27 ± 2
Resting sarcomere length (µm)	2.20 ± 0.02
Passive force (µN)	0.53 ± 0.08	0.58 ± 0.08
Passive tension (kN/m²)	1.32 ± 0.18	1.39 ± 0.14
Max force (µN)	14.0 ± 2.3	14.7 ± 2.2
Max tension (kN/m²)	30.9 ± 3.5	36.6 ± 4.6^a
k_tr @ pCa 4.5 (s⁻¹)	0.515 ± 0.04	0.666 ± 0.04^a
pCa for half-maximal force	6.06 ± 0.03	5.96 ± 0.03
Relative force (P_O/P_4.5)	0.50 ± 0.04	0.52 ± 0.04
a/P_O	0.16 ± 0.04	0.20 ± 0.07
F_opt (P/P_O)	0.25 ± 0.02	0.27 ± 0.02
V_opt (ML/s)	0.100 ± 0.012	0.117 ± 0.009
V_max	0.45 ± 0.09	0.47 ± 0.07
PNPO (P/P_O*ML/s)	0.024 ± 0.002	0.031 ± 0.002^a
Submax k_tr	0.456 ± 0.05	0.468 ± 0.05

Mechanical properties were tested before and after PKA treatment and compared via paired t test analysis. Values are mean ± SEM.

P ≤ 0.05. n equals the number of human hearts (biological replicates), with one cardiac myocyte preparation per heart.