Table 3. Parameters determined from... | Rockefeller University Press

Table 3.

Parameters determined from Boltzmann fits to the voltage dependences of steady-state F-V curves for Na_V1.5 voltage sensor domains, VSD-I–VSD-IV^{^a}

	Na_V1.5	Na_V1.5 + iFGF12B	Na_V1.5 + iFGF13VY
VSD-I (n)	(4)	(3)	(4)
V_1/2 (mV)	−75.2 ± 3.6	−77.6 ± 4.6	−50.1 ± 3.1
P value^{^b}		0.99	0.003 (**)
k	19.4 ± 0.9	21.2 ± 2.7	13.7 ± 1.2
P value^{^b}		0.97	0.008 (**)
VSD-II (n)	(5)	(4)	(5)
V_1/2 (mV)	−51.6 ± 1.8	−53.1 ± 2.9	−53.4 ± 1.7
P value^{^b}		0.99	0.25
k	33.0 ± 4.9	27.4 ± 2.0	38.7 ± 0.9
P value^{^b}		0.84	0.50
VSD-III (n)	(10)	(8)	(6)
V_1/2 (mV)	−149.7 ± 1.6	−145.6 ± 2.1	−153.4 ± 4.3
P value^{^b}		0.28	0.68
k	21.4 ± 0.8	20.6 ± 0.7	22.0 ± 2.0
P value^{^b}		0.71	0.95
VSD-IV (n)	(8)	(8)	(8)
V_1/2 (mV)	−52.9 ± 2.0	−66.2 ± 2.3	−60.4 ± 1.3
P value^{^b}		0.001 (***)	0.02 (*)
k	20.5 ± 1.3	16.5 ± 1.2	9.4 ± 1.0
P value^{^b}		0.08	<0.001 (***)

	Na_V1.5	Na_V1.5 + iFGF12B	Na_V1.5 + iFGF13VY
VSD-I (n)	(4)	(3)	(4)
V_1/2 (mV)	−75.2 ± 3.6	−77.6 ± 4.6	−50.1 ± 3.1
P value^{^b}		0.99	0.003 (**)
k	19.4 ± 0.9	21.2 ± 2.7	13.7 ± 1.2
P value^{^b}		0.97	0.008 (**)
VSD-II (n)	(5)	(4)	(5)
V_1/2 (mV)	−51.6 ± 1.8	−53.1 ± 2.9	−53.4 ± 1.7
P value^{^b}		0.99	0.25
k	33.0 ± 4.9	27.4 ± 2.0	38.7 ± 0.9
P value^{^b}		0.84	0.50
VSD-III (n)	(10)	(8)	(6)
V_1/2 (mV)	−149.7 ± 1.6	−145.6 ± 2.1	−153.4 ± 4.3
P value^{^b}		0.28	0.68
k	21.4 ± 0.8	20.6 ± 0.7	22.0 ± 2.0
P value^{^b}		0.71	0.95
VSD-IV (n)	(8)	(8)	(8)
V_1/2 (mV)	−52.9 ± 2.0	−66.2 ± 2.3	−60.4 ± 1.3
P value^{^b}		0.001 (***)	0.02 (*)
k	20.5 ± 1.3	16.5 ± 1.2	9.4 ± 1.0
P value^{^b}		0.08	<0.001 (***)

Values are means ± SEM; n = number of biological replicates (cells).

P values are obtained from a comparison to recordings from oocytes expressing Na_V1.5 alone (see Materials and methods). Significant differences are designated as *, **, and *** for P values <0.05, 0.01, and 0.001, respectively.

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