The values of K, Ka, Kb, Z, Za, and Zb at various spermine concentrations obtained from fitting the data presented in Fig. 2 D and Fig. 4 using the kinetic model from Fig. 5 C
| . | hTRPV1 . | hTRPV3 . | hTRPV4 . |
|---|---|---|---|
| K | 7.9 | 1.1 | 10.5 |
| Ka | 1.6 · 10−6 | 8.8 · 10−7 | 2.8 · 10−6 |
| Kb | 2.0 · 10−3 | 1.5 · 10−3 | 9.4 · 10−4 |
| Z | 6.8 | 3.2 | 6.0 |
| Za | 5.1 | 2.5 | 4.2 |
| Zb | 0.7 | 0.4 | 0.01 |
| . | hTRPV1 . | hTRPV3 . | hTRPV4 . |
|---|---|---|---|
| K | 7.9 | 1.1 | 10.5 |
| Ka | 1.6 · 10−6 | 8.8 · 10−7 | 2.8 · 10−6 |
| Kb | 2.0 · 10−3 | 1.5 · 10−3 | 9.4 · 10−4 |
| Z | 6.8 | 3.2 | 6.0 |
| Za | 5.1 | 2.5 | 4.2 |
| Zb | 0.7 | 0.4 | 0.01 |
Two binding sites model was used, as shown in Fig. 6 (solid lines).