Results of fitting of the Grel-V relationships for TRPV1, 3, and 4 with a sum of two Boltzmann distributions for spermine inhibition (presented in Fig. 2 D and Fig. 4)
Spermine, µM
Vmid1, mV
Vmid2, mV
Z1, e
Z2, e
TRPV1
10
−6.7
7.7
−3.6
−2.7
100
−22.9
17.1
−3.3
−2.0
1,000
−32.5
22.8
−2.9
−2.4
TRPV3
10
−27.5
50.6
−1.9
−1.3
100
−48.9
82.5
−1.8
−1.3
1,000
−62.3
-
−1.7
-
TRPV4
10
−11.9
−3.5
−2.8
−2.1
100
−26.1
9.7
−4.1
−1.6
1,000
−29.9
25.9
−4.5
−1.3
Spermine, µM
Vmid1, mV
Vmid2, mV
Z1, e
Z2, e
TRPV1
10
−6.7
7.7
−3.6
−2.7
100
−22.9
17.1
−3.3
−2.0
1,000
−32.5
22.8
−2.9
−2.4
TRPV3
10
−27.5
50.6
−1.9
−1.3
100
−48.9
82.5
−1.8
−1.3
1,000
−62.3
-
−1.7
-
TRPV4
10
−11.9
−3.5
−2.8
−2.1
100
−26.1
9.7
−4.1
−1.6
1,000
−29.9
25.9
−4.5
−1.3
TRPV3 in presence of 1 mM spermine was fitted with only one (descending) Boltzmann distribution.