Results of fitting of the Grel-V relationships for TRPV1, 3, and 4 with a sum of two Boltzmann distributions for spermine inhibition (presented in Fig. 2 D and Fig. 4)
| Spermine, µM . | Vmid1, mV . | Vmid2, mV . | Z1, e . | Z2, e . |
|---|---|---|---|---|
| TRPV1 | ||||
| 10 | −6.7 | 7.7 | −3.6 | −2.7 |
| 100 | −22.9 | 17.1 | −3.3 | −2.0 |
| 1,000 | −32.5 | 22.8 | −2.9 | −2.4 |
| TRPV3 | ||||
| 10 | −27.5 | 50.6 | −1.9 | −1.3 |
| 100 | −48.9 | 82.5 | −1.8 | −1.3 |
| 1,000 | −62.3 | - | −1.7 | - |
| TRPV4 | ||||
| 10 | −11.9 | −3.5 | −2.8 | −2.1 |
| 100 | −26.1 | 9.7 | −4.1 | −1.6 |
| 1,000 | −29.9 | 25.9 | −4.5 | −1.3 |
| Spermine, µM . | Vmid1, mV . | Vmid2, mV . | Z1, e . | Z2, e . |
|---|---|---|---|---|
| TRPV1 | ||||
| 10 | −6.7 | 7.7 | −3.6 | −2.7 |
| 100 | −22.9 | 17.1 | −3.3 | −2.0 |
| 1,000 | −32.5 | 22.8 | −2.9 | −2.4 |
| TRPV3 | ||||
| 10 | −27.5 | 50.6 | −1.9 | −1.3 |
| 100 | −48.9 | 82.5 | −1.8 | −1.3 |
| 1,000 | −62.3 | - | −1.7 | - |
| TRPV4 | ||||
| 10 | −11.9 | −3.5 | −2.8 | −2.1 |
| 100 | −26.1 | 9.7 | −4.1 | −1.6 |
| 1,000 | −29.9 | 25.9 | −4.5 | −1.3 |
TRPV3 in presence of 1 mM spermine was fitted with only one (descending) Boltzmann distribution.