Table 3.

Oligonucleotides used in this study

Oligonucleotide no.Oligonucleotide descriptionSequence
AGO190 3′ Cdc11A 25DS 5′-CTA​GCG​TAT​GAT​GCT​CAC​ATG​TTT​CAG​CCA​TTC​TTT​TCT​TGC​TTC​GAT​GCC​TGC​AGC​CAA​ACA​GTG​TTC-3′ 
AGO299 5′Cherry 11A -stop 5′-GAA​CGT​CTG​GAA​AAA​GAA​GCC​AAA​ACC​AAG​CAG​GAA​ATT​GAG​GAT​ATC​GGT​GAC​GGT​GCT​GGT​TTA​ATT​AAC-3′ 
AGO524 Cdc11A conGFP R 5′-CTA​GCG​TAT​GAT​GCT​CAC​ATG​TTT​CAG​CCA​TTC​TTT​TCT​TGC​TTC​GCA​TGA​TTA​CGC​CAA​GCT​TGC​GCA​TGA​TTA​CGC​CAA​GCT​TGC-3′ 
AGO540 Cdc11A GFP F 5′-GAA​CGT​CTG​GAA​AAA​GAA​GCC​AAA​ACC​AAG​CAG​GAA​ATT​GAG​GAT​AAA​ACG​ACG​GCC​AGT​GAA​TTC​G-3′ 
AGO1771 AgCDC11A gBlock R 5′-TCC​CGT​GCC​AAG​TAT​GTA​TC-3′ 
AGO1942 pET-duet-ScCDC12 VF 5′-CGC​ATA​ATC​GAA​ATT​AAT​ACG​ACT​C-3′ 
AGO2823 CDC11B_Tag_F 5′-GAG​ATC​GAG​GAG​CGT​CTG​GAA​AAA​GAA​GCC​AAA​ACT​AGA​GTC​GAA​ACG​ACG​GCC​AGT​GAA​TTC​GAG​CTC-3′ 
AGO2824 CDC11B_Tag_R 5′-AGC​TAC​TCT​TGT​TTT​GCG​TAA​AAG​ACC​ACG​CCA​ATA​CTT​TCT​ATC​CCA​TGA​TTA​CGC​CAA​GCT​TGC-3′ 
AGO3069 Cdc11A_Duet_NdeI_F 5′-GAT​ATA​CAT​ATG​ATG​TCC​AGT​ATC​AT-3′ 
AGO3070 Cdc11A_Duet_XhoI_R 5′-ACC​AGA​CTC​GAG​TTA​ATC​CTC​AAT​TTC​CTG​CTT​G-3′ 
AGO3102 CDC11_OVLP_F 5′-GGA​GGA​CCC​GGT​GGT-3′ 
AGO3103 CDC11_OVLP_R 5′-TAC​CAC​CGG​GTC​CTC-3′ 
AGO3135 cdc11a Thr62Ala fwd 5′-GCG​CAG​ATT​GAT​GTG​CAG​CTG​CGC​GAG​GAA-3′ 
AGO3136 cdc11a Thr62Ala rev 5′-CGC​GTC​GTC​GTT​GGG​CAG​CAT​CAC​GGT​CGT​C-3′ 
AGO3285 CDC11B 5′ pUC19 fwd 5′-AGT​GAA​TTC​GAG​CTC​GGT​ACC​CGG​GGT​GCT​TGG​TTT​CAA​CAG​CGG​AAG-3′ 
AGO3286 CDC11B 5′ pUC19 rev 5′-TGA​TAC​TGG​ACA​TTG​TAC​TGT​GTC​TTA​TCC​GCT​TCT​C-3′ 
AGO3287 CDC11A-GFP-G418 pUC19 fwd 5′-AGA​CAC​AGT​ACA​ATG​TCC​AGT​ATC​ATC​GAG​GCC​T-3′ 
AGO3288 CDC11A-GFP-G418 pUC19 rev 5′-ATA​CTT​TCT​ATC​AGA​TGA​GGC​CGT​CTT​TTG​TTG​ATA​GC-3′ 
AGO3289 CDC11B 3′ pUC19 fwd 5′-GAC​GGC​CTC​ATC​TGA​TAG​AAA​GTA​TTG​GCG​TGG​TCT​TTT​ACG-3′ 
AGO3290 CDC11B 3′ pUC19 rev 5′-GCA​TGC​CTG​CAG​GTC​GAC​TCT​AGA​GGA​CGT​CCT​CGT​TTT​ACC​GCA​G-3′ 
AGO3324 cdc11BΔ F 5′-CAA​AGC​CGA​GGA​GCC​TTA​GAG​AAG​CGG​ATA​AGA​CAC​AGT​ACA​GTG​TAT​TTA​CCA​ATA​ATG-3′ 
AGO3325 cdc11BΔ R 5′-CTC​TTG​TTT​TGC​GTA​AAA​GAC​CAC​GCC​AAT​ACT​TTC​TAT​CAG​ATG​AGG​CCG​TCT​TTT​GTT-3′ 
AGO3326 cdc11AΔ F 5′-GAG​CCG​AGT​AGC​CGC​AGA​GAA​GCG​CAT​AAG​ACG​CAA​ACA​GCG​TGT​ATT​TAC​CAA​TAA​TG-3′ 
AGO3327 cdc11AΔ R 5′-CAT​TCT​TTT​CTT​GCT​TCG​TGT​AAA​AGG​TCA​CAT​CAA​TGA​ATG​ATG​AGG​CCG​TCT​TTT​GTT-3′ 
AGO3336 CDC11A 3′ pUC19 fwd 5′-AGT​GAA​TTC​GAG​CTC​GGT​ACC​CGG​GGA​TCC​AAA​TTC​ATT​GAT​GTG​ACC​TTT​TAC​ACG​AAG-3′ 
AGO3337 CDC11A 3′ pUC19 rev 5′-AAG​CTT​GCA​TGC​CTG​CAG​GTC​GAC​TCT​AGA​TAC​ACA​ACA​GTT​ACA​TTT​GAG​TTC​TCC​AAC​G-3′ 
AGO3338 CDC11B-GFP-G418 pUC19 fwd 5′-CAG​TGA​ATT​CGA​GCT​CGG​TAC​CCG​GGC​CCA​TGG​CGG​GCA​TTA​TTG​AAG​C-3′ 
AGO3339 CDC11B-GFP-G418 pUC19 rev 5′-GTA​AAA​GGT​CAC​ATC​AAT​GAA​TTT​gGA​TGA​GGC​CGT​CTT​TTG​TTG​ATA​GC-3′ 
AGO3340 CDC11A 5′ pUC19 fwd 5′-TGA​ATT​CGA​GCT​CGG​TAC​CCG​GGC​CCA​CCG​TGT​CAC​TGC​CAC​G-3′ 
AGO3341 CDC11A 5′ pUC19 rev 5′-GCT​TCA​ATA​ATG​CCC​GCC​ATG​GGC​CGC​TGT​TTG​CGT​CTT​ATG​CGC-3′ 
Oligonucleotide no.Oligonucleotide descriptionSequence
AGO190 3′ Cdc11A 25DS 5′-CTA​GCG​TAT​GAT​GCT​CAC​ATG​TTT​CAG​CCA​TTC​TTT​TCT​TGC​TTC​GAT​GCC​TGC​AGC​CAA​ACA​GTG​TTC-3′ 
AGO299 5′Cherry 11A -stop 5′-GAA​CGT​CTG​GAA​AAA​GAA​GCC​AAA​ACC​AAG​CAG​GAA​ATT​GAG​GAT​ATC​GGT​GAC​GGT​GCT​GGT​TTA​ATT​AAC-3′ 
AGO524 Cdc11A conGFP R 5′-CTA​GCG​TAT​GAT​GCT​CAC​ATG​TTT​CAG​CCA​TTC​TTT​TCT​TGC​TTC​GCA​TGA​TTA​CGC​CAA​GCT​TGC​GCA​TGA​TTA​CGC​CAA​GCT​TGC-3′ 
AGO540 Cdc11A GFP F 5′-GAA​CGT​CTG​GAA​AAA​GAA​GCC​AAA​ACC​AAG​CAG​GAA​ATT​GAG​GAT​AAA​ACG​ACG​GCC​AGT​GAA​TTC​G-3′ 
AGO1771 AgCDC11A gBlock R 5′-TCC​CGT​GCC​AAG​TAT​GTA​TC-3′ 
AGO1942 pET-duet-ScCDC12 VF 5′-CGC​ATA​ATC​GAA​ATT​AAT​ACG​ACT​C-3′ 
AGO2823 CDC11B_Tag_F 5′-GAG​ATC​GAG​GAG​CGT​CTG​GAA​AAA​GAA​GCC​AAA​ACT​AGA​GTC​GAA​ACG​ACG​GCC​AGT​GAA​TTC​GAG​CTC-3′ 
AGO2824 CDC11B_Tag_R 5′-AGC​TAC​TCT​TGT​TTT​GCG​TAA​AAG​ACC​ACG​CCA​ATA​CTT​TCT​ATC​CCA​TGA​TTA​CGC​CAA​GCT​TGC-3′ 
AGO3069 Cdc11A_Duet_NdeI_F 5′-GAT​ATA​CAT​ATG​ATG​TCC​AGT​ATC​AT-3′ 
AGO3070 Cdc11A_Duet_XhoI_R 5′-ACC​AGA​CTC​GAG​TTA​ATC​CTC​AAT​TTC​CTG​CTT​G-3′ 
AGO3102 CDC11_OVLP_F 5′-GGA​GGA​CCC​GGT​GGT-3′ 
AGO3103 CDC11_OVLP_R 5′-TAC​CAC​CGG​GTC​CTC-3′ 
AGO3135 cdc11a Thr62Ala fwd 5′-GCG​CAG​ATT​GAT​GTG​CAG​CTG​CGC​GAG​GAA-3′ 
AGO3136 cdc11a Thr62Ala rev 5′-CGC​GTC​GTC​GTT​GGG​CAG​CAT​CAC​GGT​CGT​C-3′ 
AGO3285 CDC11B 5′ pUC19 fwd 5′-AGT​GAA​TTC​GAG​CTC​GGT​ACC​CGG​GGT​GCT​TGG​TTT​CAA​CAG​CGG​AAG-3′ 
AGO3286 CDC11B 5′ pUC19 rev 5′-TGA​TAC​TGG​ACA​TTG​TAC​TGT​GTC​TTA​TCC​GCT​TCT​C-3′ 
AGO3287 CDC11A-GFP-G418 pUC19 fwd 5′-AGA​CAC​AGT​ACA​ATG​TCC​AGT​ATC​ATC​GAG​GCC​T-3′ 
AGO3288 CDC11A-GFP-G418 pUC19 rev 5′-ATA​CTT​TCT​ATC​AGA​TGA​GGC​CGT​CTT​TTG​TTG​ATA​GC-3′ 
AGO3289 CDC11B 3′ pUC19 fwd 5′-GAC​GGC​CTC​ATC​TGA​TAG​AAA​GTA​TTG​GCG​TGG​TCT​TTT​ACG-3′ 
AGO3290 CDC11B 3′ pUC19 rev 5′-GCA​TGC​CTG​CAG​GTC​GAC​TCT​AGA​GGA​CGT​CCT​CGT​TTT​ACC​GCA​G-3′ 
AGO3324 cdc11BΔ F 5′-CAA​AGC​CGA​GGA​GCC​TTA​GAG​AAG​CGG​ATA​AGA​CAC​AGT​ACA​GTG​TAT​TTA​CCA​ATA​ATG-3′ 
AGO3325 cdc11BΔ R 5′-CTC​TTG​TTT​TGC​GTA​AAA​GAC​CAC​GCC​AAT​ACT​TTC​TAT​CAG​ATG​AGG​CCG​TCT​TTT​GTT-3′ 
AGO3326 cdc11AΔ F 5′-GAG​CCG​AGT​AGC​CGC​AGA​GAA​GCG​CAT​AAG​ACG​CAA​ACA​GCG​TGT​ATT​TAC​CAA​TAA​TG-3′ 
AGO3327 cdc11AΔ R 5′-CAT​TCT​TTT​CTT​GCT​TCG​TGT​AAA​AGG​TCA​CAT​CAA​TGA​ATG​ATG​AGG​CCG​TCT​TTT​GTT-3′ 
AGO3336 CDC11A 3′ pUC19 fwd 5′-AGT​GAA​TTC​GAG​CTC​GGT​ACC​CGG​GGA​TCC​AAA​TTC​ATT​GAT​GTG​ACC​TTT​TAC​ACG​AAG-3′ 
AGO3337 CDC11A 3′ pUC19 rev 5′-AAG​CTT​GCA​TGC​CTG​CAG​GTC​GAC​TCT​AGA​TAC​ACA​ACA​GTT​ACA​TTT​GAG​TTC​TCC​AAC​G-3′ 
AGO3338 CDC11B-GFP-G418 pUC19 fwd 5′-CAG​TGA​ATT​CGA​GCT​CGG​TAC​CCG​GGC​CCA​TGG​CGG​GCA​TTA​TTG​AAG​C-3′ 
AGO3339 CDC11B-GFP-G418 pUC19 rev 5′-GTA​AAA​GGT​CAC​ATC​AAT​GAA​TTT​gGA​TGA​GGC​CGT​CTT​TTG​TTG​ATA​GC-3′ 
AGO3340 CDC11A 5′ pUC19 fwd 5′-TGA​ATT​CGA​GCT​CGG​TAC​CCG​GGC​CCA​CCG​TGT​CAC​TGC​CAC​G-3′ 
AGO3341 CDC11A 5′ pUC19 rev 5′-GCT​TCA​ATA​ATG​CCC​GCC​ATG​GGC​CGC​TGT​TTG​CGT​CTT​ATG​CGC-3′ 
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