Summary of parameters of the fits for spHCN mutant channels
| . | GV1/2 (mV) . | n . | FV1/2 (mV) . | n . |
|---|---|---|---|---|
| wt* | −75.83 ± 0.48 | 3 | −54.70 ± 1.07 | 3 |
| QWE-3G* | 7.72 ± 1.34 | 4 | 43.97 ± 2.77 | 3 |
| QWE-3G* (cAMP) | 3 | 19.57 ± 3.52 | 3 | |
| R620E* | −83.05 ± 1.73 | 3 | −52.76 ± 2.41 | 3 |
| R620G* | −77.42 ± 0.69 | 4 | −54.75 ± 1.46 | 4 |
| R620A* | 4 | −46.83 ± 2.44 | 4 |
| . | GV1/2 (mV) . | n . | FV1/2 (mV) . | n . |
|---|---|---|---|---|
| wt* | −75.83 ± 0.48 | 3 | −54.70 ± 1.07 | 3 |
| QWE-3G* | 7.72 ± 1.34 | 4 | 43.97 ± 2.77 | 3 |
| QWE-3G* (cAMP) | 3 | 19.57 ± 3.52 | 3 | |
| R620E* | −83.05 ± 1.73 | 3 | −52.76 ± 2.41 | 3 |
| R620G* | −77.42 ± 0.69 | 4 | −54.75 ± 1.46 | 4 |
| R620A* | 4 | −46.83 ± 2.44 | 4 |
GV1/2 and FV1/2 were obtained by fitting the GV and FV curves with a single Boltzmann equation. Data are shown as mean ± SEM. n indicates the number of biological replicates of all experiments. With the application of cAMP, the GV relation of QWE-3G* channel is not fitted by a single Boltzmann equation. R620A* mutant channel shows no detectable currents.