Summary of clathrin coat growth model fits in 3T3 mouse fibroblasts.
| . | . | Curvature H(θ) . | Surface area A(θ) . | Edge length Ɛ(θ) . | Projected area Ap(θ) . |
|---|---|---|---|---|---|
| CAM | A | 90,100 ± 1,000 nm2 | 83,700 ± 1,000 nm2 | 65,800 ± 1,000 nm2 | 66,200 ± 1,000 nm2 |
| CCM | R | 115.8 ± 1.5 nm | 98.3 ± 0.7 nm | 121.1 ± 2 nm | 108.5 ± 1.5 nm |
| CoopCM | γ | (8.2 ± 0.1) 10−3 nm−1 | (7.8 ± 0.1) 10−3 nm−1 | (7.9 ± 0.1) 10−3 nm−1 | (7.8 ± 0.1) 10−3 nm−1 |
| H0 | (11.3 ± 0.1) 10−3 nm−1 | (11.2 ± 0.1) 10−3 nm−1 | (11.2 ± 0.2) 10−3 nm−1 | (11.2 ± 0.2) 10−3 nm−1 | |
| R0 | 88.8 ± 0.7 nm | 89.3 ± 0.7 nm | 89.1 ± 1.4 nm | 89.5 ± 1.3 nm | |
| A0 | 0.45 | 0.49 | 0.47 | 0.49 | |
| kon | 128.4 nm−1 | 120.8 nm−1 | 123.9 nm−1 | 121 nm−1 |
| . | . | Curvature H(θ) . | Surface area A(θ) . | Edge length Ɛ(θ) . | Projected area Ap(θ) . |
|---|---|---|---|---|---|
| CAM | A | 90,100 ± 1,000 nm2 | 83,700 ± 1,000 nm2 | 65,800 ± 1,000 nm2 | 66,200 ± 1,000 nm2 |
| CCM | R | 115.8 ± 1.5 nm | 98.3 ± 0.7 nm | 121.1 ± 2 nm | 108.5 ± 1.5 nm |
| CoopCM | γ | (8.2 ± 0.1) 10−3 nm−1 | (7.8 ± 0.1) 10−3 nm−1 | (7.9 ± 0.1) 10−3 nm−1 | (7.8 ± 0.1) 10−3 nm−1 |
| H0 | (11.3 ± 0.1) 10−3 nm−1 | (11.2 ± 0.1) 10−3 nm−1 | (11.2 ± 0.2) 10−3 nm−1 | (11.2 ± 0.2) 10−3 nm−1 | |
| R0 | 88.8 ± 0.7 nm | 89.3 ± 0.7 nm | 89.1 ± 1.4 nm | 89.5 ± 1.3 nm | |
| A0 | 0.45 | 0.49 | 0.47 | 0.49 | |
| kon | 128.4 nm−1 | 120.8 nm−1 | 123.9 nm−1 | 121 nm−1 |
Fitted parameter values for the constant area model (CAM), the constant curvature model (CCM), and the cooperative curvature model (CoopCM) when fitting curvature H(θ), surface area A(θ), edge length Ɛ(θ), and projected area Ap(θ). A: Surface area fitted with CAM; R: Radius fitted with CCM; γ: Constant rate of curvature increase fitted with CoopCM; H0: Preferred curvature of the clathrin coat fitted with CoopCM; R0: preferred radius of the clathrin coat fitted with CoopCM; A0: Fraction of surface area growing as a flat lattice before curvature initiation, defined as A0 = A(θ = 0.01)/A(θ = 180°); kon: local growth rate obtained from θ(t) fitted with CoopCM and measured in nm per pseudotime units −1. N = 7 cells; n = 688 sites.